Characterization of the Chitinase Gene Family in Mulberry (Morus notabilis) and MnChi18 Involved in Resistance to Botrytis cinerea

Xin, Youchao; Wang, Donghao; Han, Shengmei; Li, Suxia; Gong, Na; Fan, Yiting; Ji, Xiaodong

doi:10.3390/genes13010098

Cited by 22 publications

(11 citation statements)

References 46 publications

(52 reference statements)

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“…Gene Ca_26715 is an endochitinase A-like protein . Chitinase is known to protect plants against abiotic and biotic stresses ( de Las Mercedes Dana et al., 2006 ; Karlsson and Stenlid, 2008 ; Xin et al., 2021 ; Zhang et al., 2022 ). The association between endochitinase and ciceritol content in chickpea suggests a metabolic link between the ciceritol pathway and the pathway leading to biotic and abiotic resistance.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide association mapping of seed oligosaccharides in chickpea

et al. 2022

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Chickpea (Cicer arietinum L.) is one of the major pulse crops, rich in protein, and widely consumed all over the world. Most legumes, including chickpeas, possess noticeable amounts of raffinose family oligosaccharides (RFOs) in their seeds. RFOs are seed oligosaccharides abundant in nature, which are non-digestible by humans and animals and cause flatulence and severe abdominal discomforts. So, this study aims to identify genetic factors associated with seed oligosaccharides in chickpea using the mini-core panel. We have quantified the RFOs (raffinose and stachyose), ciceritol, and sucrose contents in chickpea using high-performance liquid chromatography. A wide range of variations for the seed oligosaccharides was observed between the accessions: 0.16 to 15.13 mg g-1 raffinose, 2.77 to 59.43 mg g-1 stachyose, 4.36 to 90.65 mg g-1 ciceritol, and 3.57 to 54.12 mg g-1 for sucrose. Kabuli types showed desirable sugar profiles with high sucrose, whereas desi types had high concentrations RFOs. In total, 48 single nucleotide polymorphisms (SNPs) were identified for all the targeted sugar types, and nine genes (Ca_06204, Ca_04353, and Ca_20828: Phosphatidylinositol N-acetylglucosaminyltransferase; Ca_17399 and Ca_22050: Remorin proteins; Ca_11152: Protein-serine/threonine phosphatase; Ca_10185, Ca_14209, and Ca_27229: UDP-glucose dehydrogenase) were identified as potential candidate genes for sugar metabolism and transport in chickpea. The accessions with low RFOs and high sucrose contents may be utilized in breeding specialty chickpeas. The identified candidate genes could be exploited in marker-assisted breeding, genomic selection, and genetic engineering to improve the sugar profiles in legumes and other crop species.

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Section: Resultsmentioning

confidence: 99%

Genome-wide association mapping of seed oligosaccharides in chickpea

et al. 2022

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“…To verify genes enriched in "chitin catabolic", "MAPK signaling", and "plant hormone signal transduction" pathways of plant immunity, RT-qPCR was performed to determine expression patterns of five candidate DEGs. Chitinases isolated from plants can limit the growth of chitin-containing fungi, and overexpressed chitinases in plants can resist infection of different fungal pathogens [30]. After CLBB1 infection, the expression level of CsCHITINASE III (TEA002526) was highly up-regulated at 48 hai in tea cultivar 'Shuixian' (Fig.…”

Section: Reverse-transcription Quantitative Pcr Verification Of Candi...mentioning

confidence: 99%

Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea (Camellia sinensis) in China and Specific Mechanisms of Disease Infection

Wu¹,

Rui²,

Lu³

et al. 2023

Phyton

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Gray blight disease (GBD) causes significant losses in tea production in China. Although genes and biological processes involved in resistance to fungal disease in tea plants have been identified, specific mechanisms of the GBD infection process remain unknown. In this study, morphological and multi-gene (TEF-TUB-ITS) phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis vietnamensis. Pathogenicity tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock (Camellia sinensis var. sinensis cv. Shuixian). Spores began to germinate 24 h after infection (hai), and after 48 h, elongated fungal hyphae formed from a single conidium. Transcriptome analysis revealed that 482, 517, and 369 genes were differentially expressed at 24, 48, and 72 hai, respectively, in Wuyi Rock tea leaves. Isolate CLBB1 infection elicited phenotype-related responses and activated defense-related pathways, including plant-pathogen interaction, MAPK signaling, and plant hormone signal transduction, suggesting a possible mechanism underlying phenotype-based susceptibility to CLBB1. Thus, a new Ps. vietnamensis strain causing GBD in the tea cultivar 'Shuixian' was discovered in this study. Transcriptome analysis indicated that pathogen invasion activated chitin-related MAPK pathways and that tea plants required a hormone to restrict CLBB1.

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“…Chitinase genes have critical roles in multiple processes, including plant development and defense against stress. For example, after inoculating MnChi18 transgenic Arabidopsis with Botrytis cinerea , the activity of catalase (CAT) and the amount of malondialdehyde (MDA) decreased, showing that overexpression of MnChi18 could boost the resistance to B. cinerea [ 9 ]. The transient expression of CaChiIII7 significantly induced the expression of many defense-related genes and the high expression in pepper leaves along with H 2 O 2 biosynthesis, and improved the basic resistance of pepper to C. acutatum [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…The chitinase gene family has been studied in a variety of plants, such as cotton ( Gossypium ) [ 13 ], cucumber ( Cucumis sativus ) [ 23 ], tomato ( Solanum lycopersicum ) [ 24 ], tea ( Camellia sinensis ) [ 5 ], cabbage ( Brassica oleracea ) [ 25 ], mulberry ( Morus notabilis ) [ 9 ], domesticated apple ( Malus domestica ), and wild apple ( Malus sieversii ) [ 26 ]. However, less information is available about the roles of chitinase genes in strawberry.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14

Fan

Jiao

et al. 2023

Plants

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Plant chitinases (EC 3.2.1.14) are pathogenesis-related (PR) proteins and are well studied in many plant species. However, little is known about the genomic organization and expression of chitinase genes in strawberries (Fragaria vesca). Here, 23 FvChi genes were identified in the genome of strawberry (F. vesca) and divided into GH18 and GH19 subfamilies based on phylogenetic relationships. A detailed bioinformatics analysis of the FvChi genes was performed, including gene physicochemical properties, chromosomal location, exon–intron distribution, domain arrangement, and subcellular localization. Twenty-two FvChi genes showed upregulation after Colletotrichum gloeosporioides infection. Following the exogenous application of SA, FvChi-3, 4, and 5 showed significant changes in expression. The ectopic expression of FvChi-14 in Arabidopsis thaliana increased resistance to C. higginsianum via controlling the SA and JA signaling pathway genes (AtPR1, AtICS1, AtPDF1.2, and AtLOX3). The FvChi-14 protein location was predicted in the cell wall or extracellular matrix. We speculate that FvChi-14 is involved in disease resistance by regulating the SA and JA signaling pathways. The findings of this study provide a theoretical reference for the functional studies of FvChi genes and new candidates for strawberry stress resistance breeding programs.

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Characterization of the Chitinase Gene Family in Mulberry (Morus notabilis) and MnChi18 Involved in Resistance to Botrytis cinerea

Cited by 22 publications

References 46 publications

Genome-wide association mapping of seed oligosaccharides in chickpea

Genome-wide association mapping of seed oligosaccharides in chickpea

Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea (Camellia sinensis) in China and Specific Mechanisms of Disease Infection

Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14

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