Characterization of the cholesterol crystallization-promoting low- density particle isolated from human bile

Bruijn, M. A. C. De; Mok, King-Tong; Nibbering, C. P.; Out, T. A.; Marle, J. van; Stellaard, Frans; Tytgat, G. N. J.; Groen, Albert K.

doi:10.1053/gast.1996.v110.pm8964421

Cited by 14 publications

(16 citation statements)

References 5 publications

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“…This fraction contains several proteins and lipids, an 85-kd protein being the most prominent factor. 20 To avoid the complication of interacting factors in the present study, we have chosen to extract the complete CABF. The principle of the method has been published before, 14 raised whether activity of CABF may be artifactuous, 21 we have performed a series of additional control experiments.…”

Section: Discussionmentioning

confidence: 99%

Increased Activity in the Biliary Con A-Binding Fraction Accounts for the Difference in Crystallization Behavior in Bile From Chilean Gallstone Patients Compared With Dutch Gallstone Patients

et al. 2001

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Section: Discussionmentioning

confidence: 99%

Increased Activity in the Biliary Con A-Binding Fraction Accounts for the Difference in Crystallization Behavior in Bile From Chilean Gallstone Patients Compared With Dutch Gallstone Patients

et al. 2001

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“…Alterations in activities or concentrations of these proteins, associated with diseases, in body fluids are widely used as diagnostic tools and indices of therapeutic responses (19)(20)(21)(22)(23)(24). Recently, cancer associated changes in the sugar chains of various enzymes or other proteins have been reported, suggesting that studies of alterations in sugar chains of these proteins may become a possible diagnostic tool for human diseases (18)(19)(20)(21)25).…”

Section: Introductionmentioning

confidence: 99%

Concanavalin A Binding on PHEMA Beads and Their Interactions with Myeloma Cells

Yavuz

Özden

Kin

et al. 2008

Journal of Macromolecular Science, Part A

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The aim of this study is to prepare concanavalin A (Con A) bound poly(2-hydroxy ethyl methacrylate) (PHEMA) beads for cell affinity chromatography. In the first step, PHEMA beads were produced by suspension polymerization, and activated by cyanogen bromide (CNBr) in an alkaline medium (pH 11.5), and then, the bio-ligand "Con A" was attached by covalent binding onto the CNBr activated beads. PHEMA beads were characterized by scanning electron microscopy (SEM), surface area and pore size measurements. The PHEMA beads have a spherical shape and porous structure. The specific surface area of the PHEMA beads was found to be 39.7 m 2 /g with a size range of 150-200 µm in diameter and the swelling ratio was 55%. The amount of bound Con A was controlled by changing pH and the initial concentrations of CNBr and Con A. The non-specific adsorption of Con A on the plain PHEMA beads was 0.1 mg/g. The maximum Con A binding was 4.8 mg/g at pH 7.25. Both plain and Con A bound PHEMA beads were interacted first with the myeloma cell suspension in phosphate buffer. Myeloma cell attachment was very low for the plain PHEMA beads, while the number of myeloma cells attached increased almost 20 fold when the Con A bound beads were used. In order to look at whether or not the interaction of the Con A bound PHEMA beads and myeloma cells are affected from the biological molecules and other cells in the medium. We selected sheep blood itself as the medium, and mixed with the myeloma cell suspension and changed the environment. Cell adhesion decreased but not very significantly by changing the medium from simple buffer to sheep blood.

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confidence: 99%

“…23 The 85-kd glycoprotein appeared to be the most effective promotor (accelerator) of cholesterol crystallization. 23 In an earlier study we reported the presence of CEACAM1-85 and -115 isoforms in cholesterol/phospholipid vesicles isolated from bile by gel filtration followed by density gradient ultracentrifugation. 24 In the present study we show that CEACAM1-85 is present in the low-density protein-lipid complex (LDP) described by De Bruijn et al, 23 and accounts for a significant part of the potent activity of this fraction in promoting cholesterol crystallization.…”

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confidence: 99%

Carcinoembryonic antigen-related cell adhesion molecule 1 is the 85-kilodalton pronase-resistant biliary glycoprotein in the cholesterol crystallization promoting low density protein-lipid complex

et al. 2001

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Characterization of the cholesterol crystallization-promoting low- density particle isolated from human bile

Cited by 14 publications

References 5 publications

Increased Activity in the Biliary Con A-Binding Fraction Accounts for the Difference in Crystallization Behavior in Bile From Chilean Gallstone Patients Compared With Dutch Gallstone Patients

Increased Activity in the Biliary Con A-Binding Fraction Accounts for the Difference in Crystallization Behavior in Bile From Chilean Gallstone Patients Compared With Dutch Gallstone Patients

Concanavalin A Binding on PHEMA Beads and Their Interactions with Myeloma Cells

Carcinoembryonic antigen-related cell adhesion molecule 1 is the 85-kilodalton pronase-resistant biliary glycoprotein in the cholesterol crystallization promoting low density protein-lipid complex

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