Characterization of the complete genomes of Camelus dromedarius papillomavirus types 1 and 2

Abstract

Camel papillomatosis has been described previously, but the genome of the suspected papillomavirus (PV) has not been identified. An outbreak of papillomatosis occurred in a dromedary farm of 55 animals in Sudan during August 2009. The disease was only present in young animals aged about 3-7 months, of which 44 % (11/25) were affected with lesions, mainly on the lips and lower jaw. This study reports for the first time the complete genomes of Camelus dromedarius papillomavirus types 1 (CdPV1) and 2 (CdPV2), isolated from a cauliflower-like nodule and a round oval raised nodule, respectively. Pairwise comparisons of their L1 nucleotide sequences revealed 69.2 % identity, and phylogenetic analyses suggested that these two PV types are grouped within the genus Deltapapillomavirus. Both viruses were isolated from fibropapillomas, although no putative E5 proteins homologous to that of bovine papillomavirus type 1 were identified. The genetic information will be useful for evolutionary studies of the family Papillomaviridae, as well as for the development of diagnostic methods for surveillance of the disease in dromedaries. The GenBank/EMBL/DDBJ accession numbers for complete CdPV1 and CdPV2 genome sequences are HQ912790 and HQ912791, respectively.

Sero Biochemical Profile of Crossbred Calves Naturally Infected By Bovine Papilloma Virus

“…The clinical sign, microscopic changes and gross lesions observed in the present study is well corroborated with the earlier reported data (Khalafala, 1998;Valentine, 2004;Ure 2011). Most of the cutaneous wart nodules caused by BPV-type 1 and 2 and confirmed to be positive for benign type by number of research workers (Bam et al, 2012;Pangry et al, 2010;Turk et al, 2005;Smith, 1990).…”

Lack of Methylation in the Upstream Region of Human Papillomavirus Type 6 from Aerodigestive Tract Papillomas

“…We selected one pharyngeal and nine laryngeal HPV6-positive biopsy specimens (Table 1). In order to amplify the complete HPV6 genomes, automated DNA extraction (MagNA Pure LC total nucleic acid isolation; Roche) and long-range PCR (Expand Long Template PCR system; Roche) were performed (Table 2) (27). By using long PCR products as the templates, the complete genomic sequences were obtained by using 15 sequencing primers (Table 2).…”

Investigation on papillomavirus infection in dromedary camels in Al-Ahsa, Saudi Arabia

“…The first report of papillomatosis associated with PV in dromedaries was published in 1990 (Munz et al, 1990) and showed that dromedary camels in central Somalia aged 6 months to 2 years were primarily affected. Later, cases of papillomatosis in young dromedary camels have also been reported from Kenya (Dioli and Stimmelmayr, 1992), United Arab Emirates (Wernery and Kaaden, 1995) and Sudan (Khalafalla et al, 1998;Ure et al, 2011). PVs were found associated with a 2 kg wart-like growth on the right fetlock joint of a dromedary camel in India (Sadana et al, 1980) and a corneal papilloma mass in the left eye of a 15-year old dromedary male with a history of chronic severe keratoconjuctivitis (Kilic et al, 2010).…”