Characterization of the complete mitochondrial genome of <i>Euwallacea fornicatus</i> (Eichhoff, 1868) (Coleoptera: Curculionidae: Scolytinae) and its phylogenetic implications

Wang, Liang‐Jong; Hsu, Meng-Hao; Liu, Tse-Yen; Lin, Ming-Ying; Sung, Chia–Chi

doi:10.1080/23802359.2020.1827070

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…These genomes will also be a vital resource in the diagnostics and detection of these pests. While the mitochondrial genome for both species have already been published (Guo et al, 2023; Wang et al, 2020), the ability to identify nuclear markers or sequences will be important for the diagnostics and detection (such as through eDNA techniques) of the pests. Diagnostic protocols exist for the detection of E. fornicatus through identification of the symbiont Fusarium euwallaceae (De Jager & Roets, 2022), however, the protocol operates on the detection of fungus in host plant woody tissues, which indicates that the species is already present and likely reproducing in a given locality.…”

Section: Discussionmentioning

confidence: 99%

Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes ofEuwallacea fornicatusandEuwallacea similis(Coleoptera, Curculionidae, Scolytinae)

Bickerstaff,

Walsh,

Court

et al. 2024

Preprint

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BackgroundBark and ambrosia beetles are among the most ecologically and economically damaging introduced plant pests worldwide, with life history traits including polyphagy, haplodiploidy, inbreeding polygyny and symbiosis with fungi contributing to their dispersal. Species vary in host tree ecologies, with many species attacking stressed or recently dead trees, such as the globally distributedE. similis(Ferrari). At the other end of the spectrum, the Polyphagous Shot Hole Borer (PSHB),Euwallacea fornicatus(Eichhoff), attacks over 680 host plants and is causing considerable economic damage in several countries worldwide. Despite their notoriety, publicly accessible genomic resources forEuwallaceaHopkins species are scarce, hampering better understanding of their invasive capabilities as well as modern control measures, surveillance and management.FindingsUsing a combination of long read and short read sequencing we assembled and annotated high quality (BUSCO > 98% complete) chromosome level genomes for these species. Comparative macro-synteny analysis showed that chromosome number increases in haplodiploid inbreeding species ofEuwallaceadue to fission events, reducing synteny with outbred diploid species. This suggests that life history traits can impact chromosome structure. Further, the genome ofE. fornicatushad a higher relative proportion of repetitive elements, up to 17% more, thanE. similis. We used the genome assemblies to identify the haplotype of the invasive population in Perth, Western Australia. Additionally, metagenomic analyses identified microbiota associated with both species, with fewer taxa found in association withE. fornicatuscompared toE. similis.ConclusionsComparative analyses between closely related species with varying host tree ecologies and invasive capabilities will shed light into the molecular underpinning of species invasion. Moreover, these haplodiploid genomes will also contribute to the understanding of how life history traits contribute to their genome evolution.

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Section: Discussionmentioning

confidence: 99%

Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes ofEuwallacea fornicatusandEuwallacea similis(Coleoptera, Curculionidae, Scolytinae)

Bickerstaff,

Walsh,

Court

et al. 2024

Preprint

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“… 2022 ); Euwallacea fornicatus NC_059702 (Wang et al. 2020 ); Coptodryas elegans OK539700 (Guo et al. 2022 ); Phloeotribus sp.…”

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confidence: 99%

The mitochondrial genome of Dendroctonus rufipennis (Kirby, 1837) (Coleoptera: Curculionidae: Scolytinae) and its phylogenetic analyses

Meng

Tian

Wei³

et al. 2023

Mitochondrial DNA Part B

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The mitogenome of Dendroctonus rufipennis is a circular molecule of 16,547 bp which consists of 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and a control region (GenBank accession no. NC_063906). All of 13 PCGs initiate with the standard start codon of ATN. Most PCGs used the typical stop codon ‘TAA’ or ‘TAG’, only nad5 terminated with incomplete stop codon (TA). Phylogenetic analyses within the Scolytinae were performed based on nucleotide sequences of mitochondrial PCGs. The topology showed that D. rufipenni s, D. valens , and Tomicus piniperda formed a clade, and also clustered together with Hylastes opacus , H. attenuatus , H. brunneus , and H. ater . This indicated that there is a close genetic relationship between these species.

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Chromosome Structural Rearrangements in Invasive Haplodiploid Ambrosia Beetles Revealed by the Genomes of Euwallacea fornicatus (Eichhoff) and Euwallacea similis (Ferrari) (Coleoptera, Curculionidae, Scolytinae)

Bickerstaff,

Walsh,

Court

et al. 2024

Genome Biology and Evolution

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Bark and ambrosia beetles are among the most ecologically and economically damaging introduced plant pests worldwide. Life history traits including polyphagy, haplodiploidy, inbreeding polygyny and symbiosis with fungi contribute to their dispersal and impact. Species vary in their interactions with host trees, with many attacking stressed or recently dead trees, such as the globally distributed E. similis (Ferrari). Other species, like the Polyphagous Shot Hole Borer (PSHB) Euwallacea fornicatus (Eichhoff), can attack over 680 host plants and is causing considerable economic damage in several countries. Despite their notoriety, publicly accessible genomic resources for Euwallacea Hopkins species are scarce, hampering our understanding of their invasive capabilities as well as modern control measures, surveillance and management. Using a combination of long and short read sequencing platforms we assembled and annotated high quality (BUSCO > 98% complete) pseudo-chromosome level genomes for these species. Comparative macro-synteny analysis identified an increased number of pseudo-chromosome scaffolds in the haplodiploid inbreeding species of Euwallacea compared to diploid outbred species, due to fission events. This suggests that life history traits can impact chromosome structure. Further, the genome of E. fornicatus had a higher relative proportion of repetitive elements, up to 17% more, than E. similis. Metagenomic assembly pipelines identified microbiota associated with both species including Fusarium fungal symbionts and a novel Wolbachia strain. These novel genomes of haplodiploid inbreeding species will contribute to the understanding of how life history traits are related to their evolution and to the management of these invasive pests.

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Characterization of the complete mitochondrial genome of Euwallacea fornicatus (Eichhoff, 1868) (Coleoptera: Curculionidae: Scolytinae) and its phylogenetic implications

Cited by 5 publications

References 13 publications

Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes ofEuwallacea fornicatusandEuwallacea similis(Coleoptera, Curculionidae, Scolytinae)

Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes ofEuwallacea fornicatusandEuwallacea similis(Coleoptera, Curculionidae, Scolytinae)

The mitochondrial genome of Dendroctonus rufipennis (Kirby, 1837) (Coleoptera: Curculionidae: Scolytinae) and its phylogenetic analyses

Chromosome Structural Rearrangements in Invasive Haplodiploid Ambrosia Beetles Revealed by the Genomes of Euwallacea fornicatus (Eichhoff) and Euwallacea similis (Ferrari) (Coleoptera, Curculionidae, Scolytinae)

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