Characterization of the complete mitochondrial genome of Eysarcoris aeneus (Heteroptera: Pentatomidae), with its phylogenetic analysis

Abstract

In this study, the complete 16,065 bp mitochondrial genome of Eysarcoris aeneus was determined from China using next-generation sequencing technology. It consists of 13 protein-coding genes, 22 tRNAs, 2 ribosomal RNAs, and a control region. Among them, 23 genes were located on J-strand, and the other genes (nad5, nad4, nad4L, nad1, trnQ, trnC, trnY, trnF, trnH, trnP, trnL1, trnV, 12S rRNA, and 16S rRNA) were located on the N-strand. The phylogenetic tree was constructed using MrBayes method based on the 13 protein-coding genes (PCGs) showed the present species clustered within the Pentatomidae. The complete mitogenome data would be useful for further study of Pentatomoidea and Pentatomomorpha.

Comparative Mitogenomics and Phylogenetic Analyses of Pentatomoidea (Hemiptera: Heteroptera)

“…Most PCGs began with a typical ATN codon (i.e., ATA/ATT/ATG/ATC), while the start codon of COI was TTG in all pentatomoid species, and a small number of start codons were GTG in ATP8 and ATP6 . As for stop codons, they mostly consisted of TAA or TAG, while a single T residue was used as an incomplete stop codon in COI , COII , ATP6 , COIII , ND3 , ND5 , ND6 , and ND1 , which has also been detected in other hemipterans [ 48 , 49 ]. In particular, the stop codons of COII were all incomplete, except for that in Mattiphus splendidus .…”

The complete mitochondrial genome of Eysarcoris guttigerus (Hemiptera: Pentatomidae)

“…The mitogenomic size of E. guttigerus (MN831205) is 15,368 bp. Eysarcoris guttigerus shared similar gene distribution pattern with E. aeneus (Zhao et al 2019). A total of 81 bp intergenic spacers distributed in 14 locations.…”

“…Gaps were filled using sanger sequencing. Protein-coding genes (PCGs) were identified by blasting with mitogenome of Eysarcoris aeneus (Zhao et al 2019). tRNAs were identified with MITOS2 (Bernt et al 2013), rRNAs and A þ T-rich region were determined by the bound- ary of tRNAs.…”

“…Protein-coding genes (PCGs) were identified by blasting with mitogenome of Eysarcoris aeneus (Zhao et al. 2019 ). tRNAs were identified with MITOS2 (Bernt et al.…”

The devil is in the details: Problems in DNA barcoding practices indicated by systematic evaluation of insect barcodes

“…In the phylogenetic tree of Pentatomidae reconstructed by Zhao et al (2021), this sequence was considered a sister group or closely related to Pentatoma rufipes. This sequence was also used as a reference for the entire mitochondrial genome annotation of Eysarcoris aeneus, but not used to reconstruct the phylogenetic relationship (Zhao et al, 2019).…”