Characterization of the complete mitochondrial genome of the Oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae)

Abstract

Abstract. The complete mitochondrial genome (mitogenome) of Mythimna separata (Lepidoptera: Noctuidae) was determined to be 15,329 bp, including 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and an A+T-rich region. The AT skew of this mitogenome was slightly negative and the nucleotide composition was also biased toward A+T nucleotides (81.00%). All PCGs were initiated by ATN codons, except for cytochrome c oxidase subunit 1 (cox1) gene, which was initiated by CGA. Five of the 13 PCGs have the incomplete termination codon, T or TA. All the tRNA genes displayed a typical clover-leaf structure of mitochondrial tRNA. The A+T-rich region of the mitogenome was 372 bp in length and consisted of several features common to the Noctuidae. Phylogenetic analysis confirmed the placement of M. separata within the Noctuidae.

Characterization of the complete mitochondrial genome of Spilarctia robusta (Lepidoptera: Noctuoidea: Erebidae) and its phylogenetic implications

“…These results are consistent with the conclusion of many authors, e.g. Liu et al (2015) and Lammermann et al (2016). Results of further analyses strongly support a close relationship between S. robusta and L. melli (Erebidae).…”

Characterisation and phylogenetic analysis of the complete mitochondrial genome of Ctenoplusia albostriata (Lepidoptera: Noctuidae: Plusiinae)

“…MN495624), containing the typical set of 13 protein-coding, 2 rRNA, and 22 tRNA genes, and 1 non-coding AT-rich region. Gene order was conserved and identical to most other previously sequenced Noctuidae (Timmermans et al 2014;Li et al 2015;Yao et al 2018;Huang et al 2019). The nucleotide composition of the mitogenome was biased toward A and T, with 80.9% of A þ T content (A 39.6%, T 41.3%, C 11.3%, G 7.8%).…”

Selection and evaluation of reference genes for gene expression using quantitative real‐time PCR in Mythimna separata walker (Lepidoptera: Noctuidae)

“…In recent years, RT‐qPCR has been widely used to quantify gene expression levels in the studies of M. separata , such as insecticide resistance, genome, ecological adaption (Li et al . ; Liu et al . ).…”

“…Recently, the control strategy of this pest has mainly relied on the usage of chemical insecticides (Jiang et al 2011;Rashid et al 2013) to cause serious resistance (Yun et al 2004). In recent years, RT-qPCR has been widely used to quantify gene expression levels in the studies of M. separata, such as insecticide resistance, genome, ecological adaption (Li et al 2015;Liu et al 2016).…”