Drought stress is a pivotal environmental factor impacting rice production and presents a significant challenge to sustainable agriculture worldwide. This review synthesizes the latest research advancements in the regulatory mechanisms and signaling pathways that rice employs in response to drought stress. It elaborates on the adaptive changes and molecular regulatory mechanisms that occur in rice under drought conditions. The review highlights the perception and initial transmission of drought signals, key downstream signaling networks such as the MAPK and Ca2+ pathways, and their roles in modulating drought responses. Furthermore, the discussion extends to hormonal signaling, especially the crucial role of abscisic acid (ABA) in drought responses, alongside the identification of drought-resistant genes and the application of gene-editing technologies in enhancing rice drought resilience. Through an in-depth analysis of these drought stress regulatory signaling pathways, this review aims to offer valuable insights and guidance for future rice drought resistance breeding and agricultural production initiatives.