Characterization of the DNA nucleotide sequences in the genome of red sea bream iridoviruses isolated in Korea

Jeong, Joon Bum; Jun, Lyu Jin; Yoo, Min; Kim, Myong Sug; Komisar, Jack; Jeong, Hyun Do

doi:10.1016/s0044-8486(02)00538-0

Cited by 49 publications

(23 citation statements)

References 24 publications

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“…Other iridoviruses from freshwater ornamental fish from the present study produced the same size of amplicons as that of PGIV-1; M: 100 bp DNA ladder Dis Aquat Org 78: [209][210][211][212][213][214][215] 2008 could only be performed using viruses derived from tissues of infected fish. After being challenged with PGIV-1, isolated viruses from dead fish of the 2 gourami species produced the same size amplicon and nucleotide sequence (data not shown) of PCR-amplified DNA as those of the PGIV-1 used as the inoculum, indicating that the iridovirus genome was not restricted by host species, in agreement with previous studies (Jeong et al 2003). Recently, it has been found that there is a high frequency of asymptomatic iridoviral infections, demonstrated by 2-step PCR analysis, in various marine fish species in Korea (Jeong et al 2006a).…”

supporting

confidence: 89%

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Jeong¹,

Kim²,

Jun³

et al. 2008

Dis. Aquat. Org.

102

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We examined the distribution of iridoviruses in 10 freshwater ornamental fish species hatched in Korea and imported from other Asian countries using both 1-step and 2-step polymerase chain reation (PCR). None of the 10 fish species analyzed were free of iridovirus as shown by 2-step PCR positive results, and 3 species yielded 1-step PCR positive results with associated mortality. Cloned PCR amplicons of the adenosine triphosphatase (ATPase) and major capsid protein (MCP) genes in genomic DNA of iridovirus showed the same nucleotide sequences as that of infectious spleen and kidney necrosis virus (ISKNV) isolated from the mandarinfish Siniperca chuatsi. These results indicate the presence of ISKNV disease in various ornamental fish as new host species and that the disease is widespread throughout different Asian countries including Korea, Singapore and China. Such infections were either clinical with associated mortality (and 1-step PCR positive) or asymptomatic in fish that were externally healthy (and only positive in 2-step PCR). Molecular analyses of the K2 region performed on iridovirus samples isolated from freshwater ornamental fishes revealed deletion/insertion of repetitive sequences of various lengths (42 to 339 bp), depending on the ISKNV isolates, without substitutions. Experimental infection of pearl gourami Trichogaster leeri and silver gourami T. microlepis with a tissue homogenate of pearl gourami infected by ISKNV induced 70 and 20% cumulative mortalities in the pearl and silver gourami, respectively. KEY WORDS: Iridovirus · ISKNV · Ornamental fish · Asymptomatic infection · Asian countries Resale or republication not permitted without written consent of the publisherDis Aquat Org 78: [209][210][211][212][213][214][215] 2008 (ALIV) and dwarf gourami C. lalia iridovirus (DGIV) in Japan.Infectious spleen and kidney necrosis virus (ISKNV), recently assigned to the new Megalocytivirus genus of the Iridoviridae family, based on morphological and genetic characteristics (Chinchar et al. 2005), has caused significant economic loss in the mandarinfish Siniperca chuatsi aquaculture sector of China (He et al. 2000). In studies to elucidate the transmission and host range of ISKNV, mandarinfish appears to be the only species infected naturally and the most susceptible species, followed by largemouth bass in experimental infections of 21 fish species tested (He et al. 2002). In addition, the presence of ISKNV has only been reported in China. Jeong et al. (2006a), using 2-step polymerase chain reation (PCR), reported megalocytivirus in marine fish species that were externally healthy, a condition that could be called persistent or asymptomatic infection. Moreover, investigation of largemouth bass Micropterus salmoides salmoides, using a 1-step PCR technique after the occurrence of mass mortality, has also revealed the long-term persistence of ranavirus, largemouth bass virus (LMBV), in the population (Hanson & Petrie-Hanson 2001).Although Megalocytivirus and Ranavirus are epidemiologically and g...

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supporting

confidence: 89%

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Jeong¹,

Kim²,

Jun³

et al. 2008

Dis. Aquat. Org.

102

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“…(ii) Red sea bream iridovirus is a piscine iridovirus that causes an acute and highly contagious disease in Red sea bream from Japan and Korea [61]. (iii) Rock bream iridovirus is a megalocytivirus that occurs in cultured rock bream from Korea [62].…”

Section: Megalocytivirus Genomesmentioning

confidence: 99%

Virus genomes and virus-host interactions in aquaculture animals

Zhang

Gui

2015

Sci. China Life Sci.

172

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Over the last 30 years, aquaculture has become the fastest growing form of agriculture production in the world, but its development has been hampered by a diverse range of pathogenic viruses. During the last decade, a large number of viruses from aquatic animals have been identified, and more than 100 viral genomes have been sequenced and genetically characterized. These advances are leading to better understanding about antiviral mechanisms and the types of interaction occurring between aquatic viruses and their hosts. Here, based on our research experience of more than 20 years, we review the wealth of genetic and genomic information from studies on a diverse range of aquatic viruses, including iridoviruses, herpesviruses, reoviruses, and rhabdoviruses, and outline some major advances in our understanding of virus-host interactions in animals used in aquaculture.

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“…Following the rules for nomenclature of the International Committee on the Taxonomy of Viruses, RSIV, infectious spleen and kidney necrosis virus (ISKNV) (He et al, 2000) and turbot reddish body iridovirus (TRBIV) (Shi et al, 2004) belong to the genus Megalocytivirus, have a dsDNA genome approximately 111 kb in length and form the newest genus within the family Iridoviridae (Jancovich et al, 2012). Megalocytivirus infection of fish is a serious problem in both marine cultured fish and also ornamental fish, mainly in East and South-east Asia (Jeong et al, 2003(Jeong et al, , 2008a results of a comparison of phylogenetic analyses of the major capsid protein (MCP) and ATPase among them revealed that they could be divided into three groups (Kurita & Nakajima, 2012). Furthermore, it seems that there are also differences in their biological characteristics (e.g.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of red sea bream iridovirus among organs of Japanese amberjack (Seriola quinqueradiata) exposed to cultured red sea bream iridovirus

Ito

Yoshiura

Kamaishi

et al. 2013

Journal of General Virology

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Characterization of the DNA nucleotide sequences in the genome of red sea bream iridoviruses isolated in Korea

Cited by 49 publications

References 24 publications

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes

Virus genomes and virus-host interactions in aquaculture animals

Prevalence of red sea bream iridovirus among organs of Japanese amberjack (Seriola quinqueradiata) exposed to cultured red sea bream iridovirus

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