Characterization of the Drosophila melanogaster genome at the nuclear lamina

Pickersgill, Helen; Kalverda, Bernike; Wit, Elzo de; Talhout, Wendy; Fornerod, Maarten; Steensel, Bas van

doi:10.1038/ng1852

Cited by 549 publications

(581 citation statements)

References 49 publications

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“…This study identified several hundred genes as lamin-associated genes and revealed that these genes lack active histone modifications and are late replicating (Pickersgill et al 2006).…”

Section: Association Of Genes With the Nuclear Periphery And Nuclear mentioning

confidence: 99%

Dynamics and interplay of nuclear architecture, genome organization, and gene expression

Schneider¹,

Grosschedl²

2007

Genes Dev.

368

313

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The organization of the genome in the nucleus of a eukaryotic cell is fairly complex and dynamic. Various features of the nuclear architecture, including compartmentalization of molecular machines and the spatial arrangement of genomic sequences, help to carry out and regulate nuclear processes, such as DNA replication, DNA repair, gene transcription, RNA processing, and mRNA transport. Compartmentalized multiprotein complexes undergo extensive modifications or exchange of protein subunits, allowing for an exquisite dynamics of structural components and functional processes of the nucleus. The architecture of the interphase nucleus is linked to the spatial arrangement of genes and gene clusters, the structure of chromatin, and the accessibility of regulatory DNA elements. In this review, we discuss recent studies that have provided exciting insight into the interplay between nuclear architecture, genome organization, and gene expression.

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“…This study identified several hundred genes as lamin-associated genes and revealed that these genes lack active histone modifications and are late replicating (Pickersgill et al 2006).…”

Section: Association Of Genes With the Nuclear Periphery And Nuclear mentioning

confidence: 99%

Dynamics and interplay of nuclear architecture, genome organization, and gene expression

Schneider¹,

Grosschedl²

2007

Genes Dev.

368

313

View full text Add to dashboard Cite

show abstract

“…According to this hypothesis, the dissociation of OCT1 from the nuclear envelope has been shown to be associated with collagenase gene up-regulation during the cell-aging process (Imai et al, 1997). On the other hand, two recent genome-wide localization studies revealed that the association with nuclear lamina was mostly characterized by a repressive chromatin environment, devoid of active histone marks and RNA polymerase II binding activities (Pickersgill et al, 2006;Guelen et al, 2008). Of interest, one demonstrated SRY partner protein is KRAB-O, which by means of KAP-1 is able to recruit histone deacetylase and HP1 heterochromatin protein (Peng et al, 2009).…”

Section: Subcellular Localization Of the Proteinmentioning

confidence: 99%

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Montazer-Torbati

Kocer

Auguste

et al. 2010

Developmental Dynamics

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The testis-determining gene SRY is not well-conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital-ridges and not solely in pre-Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324-3335,

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“…Electron micrographs of eukaryotic nuclei show that, in most cells, condensed heterochromatin is enriched at the nuclear periphery (Reik, 2007;Ueda et al, 2014;Wu et al, 2005). Genome-wide mapping using DamID technology (Guelen et al, 2008;Pickersgill et al, 2006) or ChIP-seq (Sadaie et al, 2013;Shah et al, 2013, reviewed in Gruenbaum andFoisner, 2015) revealed that there are laminaassociated domains (LADs), genome regions of low gene density that are in contact with the lamina. In agreement with the characteristics of LADs as transcriptionally silent environments, repressive epigenetic histone modifications are commonly found at the nuclear periphery, including H3K9me2, H3K9me3, H3K27me2 and H3K27me3 (Eberhart et al, 2013;Kind et al, 2013;Wu et al, 2005;Yokochi et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data

Fišerová

Efenberková

Sieger

et al. 2017

Journal of Cell Science

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The nuclear periphery (NP) plays a substantial role in chromatin organization. Heterochromatin at the NP is interspersed with active chromatin surrounding nuclear pore complexes (NPCs); however, details of the peripheral chromatin organization are missing. To discern the distribution of epigenetic marks at the NP of HeLa nuclei, we used structured illumination microscopy combined with a new MATLAB software tool for automatic NP and NPC detection, measurements of fluorescent intensity and statistical analysis of measured data. Our results show that marks for both active and nonactive chromatin associate differentially with NPCs. The incidence of heterochromatin marks, such as H3K27me2 and H3K9me2, was significantly lower around NPCs. In contrast, the presence of marks of active chromatin such as H3K4me2 was only decreased very slightly around the NPCs or not at all (H3K9Ac). Interestingly, the histone demethylases LSD1 (also known as KDM1A) and KDM2A were enriched within the NPCs, suggesting that there was a chromatinmodifying mechanism at the NPCs. Inhibition of transcription resulted in a larger drop in the distribution of H1, H3K9me2 and H3K23me2, which implies that transcription has a role in the organization of heterochromatin at the NP.

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Characterization of the Drosophila melanogaster genome at the nuclear lamina

Cited by 549 publications

References 49 publications

Dynamics and interplay of nuclear architecture, genome organization, and gene expression

Dynamics and interplay of nuclear architecture, genome organization, and gene expression

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data

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