Characterization of the Dynamic Changes of Ruminal Microbiota Colonizing Citrus Pomace Waste during Rumen Incubation for Volatile Fatty Acid Production

Yu, Shiqiang; Li, Liuxue; Zhao, Huiying; Yan, Tiebin; Liu, Ming; Jiang, Linshu; Zhao, Yuxing

doi:10.1128/spectrum.03517-22

Cited by 7 publications

(6 citation statements)

References 55 publications

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“…Expectedly, pH was reduced by 0.03 unit with the FA inclusion without compromising the TVFA concentration. This contradicts the assumption that a reduction in the rumen pH could result in VFA accumulation, thereby limiting microbial fermentation [18]. The undegraded DM and IVTDDM values were significantly lower than the control but are similar among the EOB and EFA treatments.…”

Section: In Vitro Digestibility and Fermentation Parametersmentioning

confidence: 66%

“…Ruminal fluid pH is a critical index to evaluate microbial fermentation activity, because most ruminal cellulolytic bacteria are pH sensitive [18]. In this study, the rumen pH (6.61-6.69) was influenced by both the EOB inclusion and the EOB × FA interaction.…”

Section: In Vitro Digestibility and Fermentation Parametersmentioning

confidence: 89%

“…Remling et al [4] noted that propionate and butyrate also stimulate the growth of rumen papillae, thereby increasing the absorption surface for ruminants. Branched-chain VFA (BCVFA), such as isobutyrate, isovalerate, valerate, and 2-methylbutyric acid, are by-products of amino acid deamination in the rumen which are utilized by ruminal microbes as a source of carbon skeleton to synthesize branchedchain amino acids [18]. Higher valerate and isovalerate concentration in EFA1 and EFA4, respectively, indicates that such treatments would enhance cellulolytic bacteria population and fiber digestibility in the rumen.…”

Section: Volatile Fatty Acid Productionmentioning

confidence: 99%

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Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration

Alabi,

Okedoyin,

Anotaenwere

et al. 2023

Ruminants

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The growing interest in improving rumen fermentation and mitigating methane emissions necessitates the use of essential oil blends (EOB) and fumaric acid (FA). This study evaluated the synergistic effect of four EOB with or without FA supplementation on in vitro dry matter digestibility, greenhouse gas emission, and total volatile fatty acid production using inoculum from three rumen-cannulated Black Angus beef cows. The study was arranged in a 4 × 2 + 1 factorial design to evaluate the effects of the four EOB and two FA levels on a total mixed ration (TMR). The EOB dosage was 100 µL while FA was added at 3% of total mixed ration. The EOB × FA interaction (p < 0.05) influenced the dry matter, neutral detergent fiber, and hemicellulose degradabilities. All the EOB and FA (EFA) treatments decreased (p < 0.001) the dry matter degradability compared to the control (TMR substrate only). The EFA4 treatment reduced the neutral detergent fiber and hemicellulose degradabilities compared to the control. The ruminal pH was influenced (p < 0.001) by both the EOB and FA inclusion, and the EOB × FA interaction was significant. The microbial mass was higher (p < 0.001) in the EFA1, EFA4, and EOB4 compared to the control and the EOB3 treatments. The EFA1 and EOB1 produced less (p < 0.001) gas than the control by 29.1 and 32.1%, respectively. Compared with the control, the EFA1 and EOB1 treatments decreased (p < 0.001) methane gas by 90.8% and 86.4%, respectively, while the carbon dioxide was reduced (p = 0.004) by 65.7 and 57.9%, respectively. The EOB × FA interaction was significant (p < 0.001) for the total and individual volatile fatty acid concentrations. The inclusion of FA increased the propionate concentration by 9.5% and decreased (p = 0.02) the acetate concentration by 4%. In summary, the synergistic effect of the EOB and FA offers an effective way to reduce greenhouse gas emission and enhance total volatile fatty acids.

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Section: In Vitro Digestibility and Fermentation Parametersmentioning

confidence: 66%

Section: In Vitro Digestibility and Fermentation Parametersmentioning

confidence: 89%

Section: Volatile Fatty Acid Productionmentioning

confidence: 99%

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Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration

Alabi,

Okedoyin,

Anotaenwere

et al. 2023

Ruminants

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“…Similarly, another meta-analysis including 34 in vivo experiments confirmed that essential oil had no effect on ruminal pH, regardless of the inclusion level [34]. Ruminal fluid pH is a crucial factor influencing microbial fermentation activity and metabolism in the rumen [35].…”

Section: Discussionmentioning

confidence: 87%

A Mixture of Prebiotics, Essential Oil Blends, and Onion Peel Did Not Affect Greenhouse Gas Emissions or Nutrient Degradability, but Altered Volatile Fatty Acids Production in Dairy Cows Using Rumen Simulation Technique (RUSITEC)

Alabi,

Wuaku,

Anotaenwere

et al. 2024

Fermentation

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This study evaluated the synergistic effects of prebiotics containing galacto-oligosaccharides (GOS) and/or mannan oligosaccharides (MOS), essential oil blend (EOB), and onion peel (OPE) on fermentation characteristics using the rumen simulation technique (RUSITEC) system. Three rumen-cannulated, non-lactating Holstein Friesian cows were the inoculum donors. The substrate used for the study was a total mixed ration (TMR), which consisted of corn silage, alfalfa hay, and concentrate at 6:2:2, respectively. Sixteen fermentation vessels were randomly allotted to four treatments with four replicates each over a 9-day period in a completely randomized design. The treatments assessed include: control [TMR only], GEO [TMR + GOS + EOB + OPE], MEO [TMR + MOS + EOB + OPE], and OLEO [TMR + OLG + EOB + OPE]. OLG comprises GOS and MOS in equal proportion. EOB was included at 3 µL/g, while OPE, GOS, MOS, and OLG were added at 30 mg/g TMR. Results showed that pH, gas volume, effluent volume, and ammonia-N were not affected (p > 0.05) by the different additives. Similarly, greenhouse gas (GHG) emissions and nutrient digestibility were not affected by the treatments. Compared to the control, total volatile fatty acids (VFA) were decreased (p < 0.05) by 14.8, 10.8, and 8.5% with GEO, MEO, and OLEO inclusion, respectively, while the molar proportion of acetate was increased (p = 0.011) by 3.3, 1.1, and 3.8% with GEO, MEO, and OLEO inclusion, respectively. MEO increased isobutyrate (p = 0.001) and branched chain VFA (p = 0.013) contents; however, GEO and OLEO inclusion reduced them. Overall, the interaction of EOB, OPE, GOS, and/or MOS did not affect nutrient digestibility or GHG emissions but reduced VFA production. Further research is recommended to assess the dose effect of the additives on GHG emissions and VFA production; and to determine the long-term effects of these interventions on the rumen microbiome and animal performance.

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“…In addition, Dikdik had the highest abundance of Atopostipes compared to the other animals. In fact, previous studies show that members of the genus Rikenellaceae_RC9_gut_group (phy-lum: Bacteroidota) are associated with the primary or secondary degradation of structural carbohydrates [64], but they also play vital roles in the colonization and decomposition of biomass composed mainly of pectin, cellulose, and hemicellulose [65,66]. Members of the Prevotellaceae family (genus: Prevotella) are fiber-degrading bacteria and are usually present in high abundance.…”

Section: Microbiome Profile and Bioaugmentation Potentialmentioning

confidence: 99%

The Stool Microbiome in African Ruminants: A Comparative Metataxonomic Study Suggests Potential for Biogas Production

Vogel,

Carlotto,

Wang

et al. 2024

Fermentation

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Lignocellulosic biomass is a promising substrate for anaerobic digestion (AD) in renewable energy generation but presents a significant challenge during the hydrolysis stage of conventional AD due to the recalcitrant nature of this biomass substrate. Rumen fluid is often employed as a bioaugmentation seed to enhance hydrolysis in the AD of lignocellulosic substrates due to its richness in hydrolytic bacteria. However, using rumen fluid to enhance AD processes presents substantial hurdles, including the procurement difficulties associated with rumen fluid and ethical concerns. In this study, the fecal microbiota of 10 African ruminant species from a large zoological park (Bioparc) in Valencia, Spain, were studied using 16S rRNA gene amplicon sequencing. In this study, the fecal microbiota of 10 African ruminant species from a large zoological park (Bioparc) in Valencia, Spain, were studied using 16S rRNA gene amplicon sequencing. The investigation revealed potential similarities between the fecal microbiota from the African ruminants’ and cows’ rumen fluids, as suggested by theoretical considerations. Although direct comparative analysis with cow rumen fluid was not performed in this study, the theoretical framework and existing literature hint at potential similarities. According to our results, the Impala, Blesbok, Dikdik and Bongo ruminant species stood out as having the greatest potential to be used in bioaugmentation strategies. Key genera such as Fibrobacter, Methanobrevibacter, and Methanosphaera in Impala samples suggested Impala rumen fluid’s involvement in cellulose breakdown and methane production. Blesbok and Dikdik exhibited a high abundance of Bacillus and Atopostipes, potentially contributing to lignin degradation. The richness of Prevotellaceae and Rikenellaceae in the Bongo fecal samples is probably associated with structural carbohydrate degradation. Taken together, our results shed light on the microbial ecology of the gut contents of a whole set of Bovidae ruminants and contribute to the potential application of gut microbiota in AD.

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Characterization of the Dynamic Changes of Ruminal Microbiota Colonizing Citrus Pomace Waste during Rumen Incubation for Volatile Fatty Acid Production

Cited by 7 publications

References 55 publications

Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration

Essential Oil Blends with or without Fumaric Acid Influenced In Vitro Rumen Fermentation, Greenhouse Gas Emission, and Volatile Fatty Acids Production of a Total Mixed Ration

A Mixture of Prebiotics, Essential Oil Blends, and Onion Peel Did Not Affect Greenhouse Gas Emissions or Nutrient Degradability, but Altered Volatile Fatty Acids Production in Dairy Cows Using Rumen Simulation Technique (RUSITEC)

The Stool Microbiome in African Ruminants: A Comparative Metataxonomic Study Suggests Potential for Biogas Production

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