Characterization of the dynamic shear properties of hyaline cartilage using high‐frequency dynamic MR elastography

Lopez, Orlando; Amrami, Kimberly K.; Manduca, Armando; Ehman, Richard L.

doi:10.1002/mrm.21474

Cited by 48 publications

(54 citation statements)

References 55 publications

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“…Future work will extend the analysis of insertion site structure-function relationships to evaluation of the tensile and shear properties of the interface. These material properties can be determined by building on our ultrasound elastography analysis of the interface (21) as well as by using other functional imaging modalities such as magnetic resonance elastography (MRE) (35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the structure–function relationship at the ligament-to-bone interface

Moffat

Sun

Pena

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

231

196

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Soft tissues such as ligaments and tendons integrate with bone through a fibrocartilaginous interface divided into noncalcified and calcified regions. This junction between distinct tissue types is frequently injured and not reestablished after surgical repair. Its regeneration is also limited by a lack of understanding of the structure-function relationship inherent at this complex interface. Therefore, focusing on the insertion site between the anterior cruciate ligament (ACL) and bone, the objectives of this study are: (i ) to determine interface compressive mechanical properties, (ii ) to characterize interface mineral presence and distribution, and (iii ) to evaluate insertion site-dependent changes in mechanical properties and matrix mineral content. Interface mechanical properties were determined by coupling microcompression with optimized digital image correlation analysis, whereas mineral presence and distribution were characterized by energy dispersive x-ray analysis and backscattered scanning electron microscopy. Both region-and insertion-dependent changes in mechanical properties were found, with the calcified interface region exhibiting significantly greater compressive mechanical properties than the noncalcified region. Mineral presence was only detectable within the calcified interface and bone regions, and its distribution corresponds to region-dependent mechanical inhomogeneity. Additionally, the compressive mechanical properties of the tibial insertion were greater than those of the femoral. The interface structurefunction relationship elucidated in this study provides critical insight for interface regeneration and the formation of complex tissue systems.anterior cruciate ligament ͉ calcium phosphate ͉ fibrocartilage ͉ insertion site ͉ compression

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Section: Discussionmentioning

confidence: 99%

Characterization of the structure–function relationship at the ligament-to-bone interface

Moffat

Sun

Pena

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

231

196

View full text Add to dashboard Cite

show abstract

“…In terms of pixel-by-pixel mechanical characterization, displacement-sensitive stimulated-echo acquisition mode (STEAM) has measured deformations and strains in articular cartilage [73]. MR elastography [74] can determine the shear properties of articular cartilage [25] and measure changes that occur with enzymatic degradation [24]. Displacement encoding by stimulated echoes can be synchronized to cyclic loading [66,75] for the measurement of displacements under applied loading with MRI (dualMRI) in any MRI-visible biomaterial (figure 3b).…”

Section: Magnetic Resonance Imaging To Quantify Mechanical Changes Wimentioning

confidence: 99%

“…STEAM is constrained by long imaging times and low resolution, which becomes a major limitation with thinner tissues such as articular cartilage. Propagating high-frequency shear [24,25] or compression [73] waves indirectly to tissue within intact joints for MR elastography and STEAM, respectively, is technically difficult and requires additional equipment (i.e. highfrequency actuators).…”

Section: Magnetic Resonance Imaging To Quantify Mechanical Changes Wimentioning

confidence: 99%

Probing articular cartilage damage and disease by quantitative magnetic resonance imaging

Chan

Neu

2013

J. R. Soc. Interface.

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Osteoarthritis (OA) is a debilitating disease that reflects a complex interplay of biochemical, biomechanical, metabolic and genetic factors, which are often triggered by injury, and mediated by inflammation, catabolic cytokines and enzymes. An unmet clinical need is the lack of reliable methods that are able to probe the pathogenesis of early OA when disease-rectifying therapies may be most effective. Non-invasive quantitative magnetic resonance imaging (qMRI) techniques have shown potential for characterizing the structural, biochemical and mechanical changes that occur with cartilage degeneration. In this paper, we review the background in articular cartilage and OA as it pertains to conventional MRI and qMRI techniques. We then discuss how conventional MRI and qMRI techniques are used in clinical and research environments to evaluate biochemical and mechanical changes associated with degeneration. Some qMRI techniques allow for the use of relaxometry values as indirect biomarkers for cartilage components. Direct characterization of mechanical behaviour of cartilage is possible via other specialized qMRI techniques. The combination of these qMRI techniques has the potential to fully characterize the biochemical and biomechanical states that represent the initial changes associated with cartilage degeneration. Additionally, knowledge of in vivo cartilage biochemistry and mechanical behaviour in healthy subjects and across a spectrum of osteoarthritic patients could lead to improvements in the detection, management and treatment of OA.

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“…The main issue with high-frequency excitation is the strong damping of shear waves. Using a 5-kHz excitation frequency, Lopez et al 120 were able to measure the mean shear stiffness of 8-mm-thick natural bovine hyaline cartilage plugs and found it to be on the order of 2 MPa. In this study, they also reported a decrease in tissue stiffness following selective enzymatic degradation, which released the proteoglycans from the cartilage.…”

Section: Magnetic Resonance Elastographymentioning

confidence: 99%

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

Kotecha

Klatt

Magin

2013

Tissue Engineering Part B: Reviews

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A key technical challenge in cartilage tissue engineering is the development of a noninvasive method for monitoring the composition, structure, and function of the tissue at different growth stages. Due to its noninvasive, three-dimensional imaging capabilities and the breadth of available contrast mechanisms, magnetic resonance imaging (MRI) techniques can be expected to play a leading role in assessing engineered cartilage. In this review, we describe the new MR-based tools (spectroscopy, imaging, and elastography) that can provide quantitative biomarkers for cartilage tissue development both in vitro and in vivo. Magnetic resonance spectroscopy can identify the changing molecular structure and alternations in the conformation of major macromolecules (collagen and proteoglycans) using parameters such as chemical shift, relaxation rates, and magnetic spin couplings. MRI provides high-resolution images whose contrast reflects developing tissue microstructure and porosity through changes in local relaxation times and the apparent diffusion coefficient. Magnetic resonance elastography uses low-frequency mechanical vibrations in conjunction with MRI to measure soft tissue mechanical properties (shear modulus and viscosity). When combined, these three techniques provide a noninvasive, multiscale window for characterizing cartilage tissue growth at all stages of tissue development, from the initial cell seeding of scaffolds to the development of the extracellular matrix during construct incubation, and finally, to the postimplantation assessment of tissue integration in animals and patients.

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Characterization of the dynamic shear properties of hyaline cartilage using high‐frequency dynamic MR elastography

Cited by 48 publications

References 55 publications

Characterization of the structure–function relationship at the ligament-to-bone interface

Characterization of the structure–function relationship at the ligament-to-bone interface

Probing articular cartilage damage and disease by quantitative magnetic resonance imaging

Monitoring Cartilage Tissue Engineering Using Magnetic Resonance Spectroscopy, Imaging, and Elastography

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