Characterization of the Effects of 3-Aminopropyl Triethoxysilane on Stability, Optical Properties and Morphology of Colloidal Gold Nanoparticles

Farrell, Monique; Rutherford, Gugu; Pradhan, A. K.

doi:10.2174/1877946806999160331182218

Cited by 2 publications

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“…We have developed a unified method for the visual detection of denatured forms of proteins utilizing concentration-dependent aggregation of AuNps via APTES. From previously published work, it is known that the addition of an aminosilane to gold nanoparticles will induce extensive shifts in the absorbance spectra and can be exploited to visually detect stable proteins [22,23]. With the target goal of ferritin visual detection, a highly sensitivity experiment was employed to confirm viability of ferritin for the proposed study.…”

Section: Resultsmentioning

confidence: 99%

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

et al. 2019

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The presence of denatured proteins within a therapeutic drug product can create a series of serious adverse effects, such as mild irritation, immunogenicity, anaphylaxis, or instant death to a patient. The detection of protein degradation is complicated and expensive due to current methods associated with expensive instrumentation, reagents, and processing time. We have demonstrated here a platform for visual biosensing of denatured proteins that is fast, low cost, sensitive, and user friendly by exploiting the plasmonic properties of noble metal nanoparticles. In this study we have exposed artificially heat stressed ferritin and gold nanoparticles to 3-aminopropyl triethoxysilane, which degrades the protein by showing a systematic blue shift in the absorbance spectra of the gold nanoparticle/ferritin and aminosilane solution. This blue shift in absorbance produces a detectable visual color transition from a blue color to a purple hue. By studying the Raman spectroscopy of the gold nanoparticle/ferritin and aminosilane solution, the extent of ferritin degradation was quantified. The degradation of ferritin was again confirmed using dynamic light scattering and was attributed to the aggregation of the ferritin due to accelerated heat stress. We have successfully demonstrated a proof of concept for visually detecting ferritin from horse spleen that has experienced various levels of degradation, including due to heat stress.

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Section: Resultsmentioning

confidence: 99%

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

et al. 2019

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Visual Detection of Biomolecules Using Concentration Dependent Induced Aggregation of Plasmonic Gold Nanoparticles

Farrell

Pradhan

2022

Micro

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Significant advancement has occurred in the detection methods of solution-based analytes. High-pressure liquid chromatography, gas chromatography, and other systems used for analyses are quite expensive. Therefore, there is a need for new methods and for the visible detection of analytes. Here, we demonstrate that 3-aminopropyl triethoxysilane (APTES) could impact the stability, optical, and morphology of gold nanoparticles (AuNps) in a colloidal solution. These impacts can be used to create a sensitive visual detection system. The strong impact of the APTES concentration on the ultraviolet–visible absorption spectra of the solutions is illustrated, which displays systematic and extensive red shifts. The presence of denatured proteins within a therapeutic drug product can induce a series of adverse effects. This report describes a fast, low cost, sensitive, and user-friendly platform where the plasmonic nanoparticles create visual biosensing of denatured proteins. Artificially heat stressed ferritin, glutathione, and insulin coupled to AuNps are exposed to ATES and upon denaturation of the protein or peptide, systematic blue or red shifts are observed in the absorbance spectra of the AuNps/biomolecules, and aminosilane solution. This serves as a proof-of-concept for a fast in-solution detection method for heat-stressed proteins or peptides.

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Characterization of the Effects of 3-Aminopropyl Triethoxysilane on Stability, Optical Properties and Morphology of Colloidal Gold Nanoparticles

Cited by 2 publications

References 0 publications

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

Visual Detection of Biomolecules Using Concentration Dependent Induced Aggregation of Plasmonic Gold Nanoparticles

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