Characterization of the eg95 gene family in the G6 genotype of Echinococcus granulosus

Rojas, Cristian A. Álvarez; Gauci, Charles G.; Nolan, Matthew J.; Harandi, Majid Fasihi; Lightowlers, Marshall W.

doi:10.1016/j.molbiopara.2012.02.005

Cited by 12 publications

(15 citation statements)

References 49 publications

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“…The G6 genotype has also been described as the cause of human infections across diverse regions of the world, including Argentina , Austria , Chile , Iran , Kenya , Mauritania and Poland . The genotypes G5 and G8 have also been identified as causing human infections, although they are regarded as substantially less important in the epidemiology of cystic echinococcosis compared with the G1 and G6 genotypes.…”

Section: Brief Definitive Reportmentioning

confidence: 99%

“…Whether the current EG95 vaccine, EG95‐1‐G1, based on nomenclature by Alvarez et al . would protect against infections with other E. granulosus genotypes is unknown. In particular, it may be useful to be able to control transmission of E. granulosus G6 in areas where this genotype causes significant levels of human disease.…”

Section: Brief Definitive Reportmentioning

confidence: 99%

“…There have been some attempts made to define this diversity . However, most of these data have been obtained using PCR‐based methods with primers that were not gene specific, such that the data cannot be relied upon as an accurate reflection of EG95‐related gene(s) and protein(s) sequences . Recently, the EG95‐related gene family in the G6 genotype of E. granulosus was defined accurately using genomic DNA cloning and conventional sequencing methods .…”

Section: Brief Definitive Reportmentioning

confidence: 99%

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Antigenic differences between the EG95‐related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination

Rojas

Gauci

Lightowlers

2013

Parasite Immunology

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Cystic echinococcosis caused by Echinococcus granulosus remains an important and neglected issue in public health. The study of the likely efficacy of the currently available EG95 vaccine against other genotypes of the parasite is important to improve the vaccine as a potential tool to be used in control programmes. The recombinant vaccine EG95-1G1 was developed based on the G1 genotype of E. granulosus. Characterization of the eg95 gene family in the G6 genotype by genomic DNA cloning previously produced the first unequivocal information about the composition of the gene family in a different genotype. The information was used in this study to predict and express two EG95-related proteins from the G6 genotype as recombinants, for assessment of their capacity to bind antibodies raised in sheep vaccinated with the EG95-1G1 vaccine. The proteins (EG95-1G6 and EG95-5G6) from the G6 genotype of E. granulosus were unable to bind all the antibodies raised by sheep vaccinated with EG95-1G1. Differences in the amino acid sequence of EG95-related proteins from G6 and likely the differences in the encoded FnIII domain may be responsible for changes in the conformation of these epitopes.

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Section: Brief Definitive Reportmentioning

confidence: 99%

Section: Brief Definitive Reportmentioning

confidence: 99%

Section: Brief Definitive Reportmentioning

confidence: 99%

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Antigenic differences between the EG95‐related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination

Rojas

Gauci

Lightowlers

2013

Parasite Immunology

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“…The currently most promising vaccine candidate EG95-1(G1) [44] differs also from its homologue in the E. canadensis (G6) by few AA variations (5 AA substitutions), but these substitutions result in conformational changes, which lead to a distinct difference in antigenicity between EG95-1G1 and EG95-1G6 [25], [26]. Indeed, antibodies from sheep vaccinated with EG95-1G1 did not react with EG95-1G6 antigen from E. canadensis (G6) [26].…”

Section: Discussionmentioning

confidence: 99%

“…Variation neutrality was challenged for EG95 genes [22] and theoretically calculated data indicated a genetic polymorphism of the EG95 antigen-coding genes among different genetically characterized strains of E. granulosus [23]. Rojas et al [25], [26] showed that this variability affects the antigenicity of EG95 antigen, by demonstrating that recombinant protein EG95-1G6 ( E. canadensis (G6)) was not recognized by antibodies raised by sheep vaccinated with EG95-1G1. Genetic polymorphism in diagnostic/vaccine candidates was also reported for other parasites including protozoa (e.g.…”

Section: Introductionmentioning

confidence: 99%

Echinococcus P29 Antigen: Molecular Characterization and Implication on Post-Surgery Follow-Up of CE Patients Infected with Different Species of the Echinococcus granulosus Complex

et al. 2014

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The protein P29 is a potential serological marker for post-treatment monitoring of cystic echinococcosis (CE) especially in young patients. We now have demonstrated that P29 is encoded in the Echinococcus genus by a single gene consisting of 7 exons spanning 1.2 kb of DNA. Variability of the p29 gene at inter- and intra-species level was assessed with 50 cDNA and 280 genomic DNA clones isolated from different E. granulosus s.l. isolates (E. granulosus sensu stricto (G1), E. equinus (G4), E. ortleppi (G5), E. canadensis (G6), E. canadensis (G7) and E. canadensis (G10)) as well as four E. multilocularis isolates. Scarce interspecies polymorphism at the p29 locus was observed and affected predominantly E. granulosus s.s. (G1), where we identified two alleles (A1 and A2) coding for identical P29 proteins and yielding in three genotypes (A1/A1, A2/A2 and A1/A2). Genotypic frequencies expected under Hardy-Weinberg equilibrium revealed a high rate of heterozygosity (47%) that strongly supports the hypothesis that E. granulosus s.s. (G1) is predominantly outbreeding. Comparative sequence analyses of the complete p29 gene showed that phylogenetic relationships within the genus Echinococcus were in agreement with those of previous nuclear gene studies. At the protein level, the deduced P29 amino acid (AA) sequences exhibited a high level of conservation, ranging from 97.9% AA sequence identity among the whole E. granulosus s.l. group to 99.58% identity among E. multilocularis isolates. We showed that P29 proteins of these two species differ by three AA substitutions without implication for antigenicity. In Western-blot analyses, serum antibodies from a human CE patient infected with E. canadensis (G6) strongly reacted with recombinant P29 from E. granulosus s.s. (G1) (recEg(G1)P29). In the same line, human anti-Eg(G1)P29 antibodies bound to recEcnd(G6)P29. Thus, minor AA sequence variations appear not to impair the prognostic serological use of P29.

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Development of Vaccine to Stop Endemic of Hydatid Disease and Promote Connectivity of the Belt and Road Initiative

Zhang,

Liu

2023

Parasitology Research Monographs

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Characterization of the eg95 gene family in the G6 genotype of Echinococcus granulosus

Cited by 12 publications

References 49 publications

Antigenic differences between the EG95‐related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination

Antigenic differences between the EG95‐related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination

Echinococcus P29 Antigen: Molecular Characterization and Implication on Post-Surgery Follow-Up of CE Patients Infected with Different Species of the Echinococcus granulosus Complex

Development of Vaccine to Stop Endemic of Hydatid Disease and Promote Connectivity of the Belt and Road Initiative

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