Characterization of the epidermal growth factor receptor in preimplantation pig conceptuses

Zhang, Y.; Paria, Bibhash C.; Dey, Sudhansu K.; Davis, Duane L.

doi:10.1016/0012-1606(92)90199-q

Cited by 19 publications

(6 citation statements)

References 35 publications

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“…Particularly prominent appeared to be the phosphotyrosine content of 42-, 116-, and 170-kDa proteins. As expected, based on the size of pig EGFR [27], the 170-kDa tyrosine phosphorylated protein exactly comigrated with the signal of the EGFR (Fig. 2A, bottom).…”

Section: Egf Induces Tyrosine Phosphorylation Of Egfr and Other Cumulsupporting

confidence: 50%

Epidermal Growth Factor-Receptor Tyrosine Kinase Activity Regulates Expansion of Porcine Oocyte-Cumulus Cell Complexes In Vitro1

Procházka

Kaláb

Nagyová

2003

Biology of Reproduction

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We have recently shown that epidermal growth factor (EGF) strongly stimulates expansion of porcine oocyte-cumulus complexes (OCCs) isolated from large follicles (>6 mm) and does not promote expansion of OCCs from small (3-4-mm) follicles. In order to elucidate the role of EGF in OCCs expansion, in the present study, we first examined the presence of EGF receptors (EGFRs) in cumulus cells isolated from follicles of different sizes. Surprisingly, immunoblotting showed that cumulus cells obtained from all follicular size categories contained similar amounts of EGFR protein. On the other hand, we found a dramatic difference in the pattern of protein tyrosine phosphorylation in a comparison of cumulus cells isolated from small and large follicles treated by EGF. Furthermore, tyrosine-phosphorylated EGFR was specifically immunoprecipitated with antiphosphotyrosine antibodies from EGF-treated cumulus cells isolated from the large follicles. This result strongly indicates that only OCCs from the large follicles contain mature EGFRs that are capable of becoming activated by EGF. Remarkably, preincubation of cumulus cells from small follicles (3-4 mm) with FSH strongly increased EGF-stimulated tyrosine phosphorylation to levels comparable with OCCs from large follicles. The FSH-dependent activation of EGFRs was beneficial for expansion of OCCs isolated from the small follicles since OCCs treated sequentially by FSH (3 h) and EGF (1 h) underwent expansion significantly better then OCCs cultured in FSH or EGF alone. We conclude that a FSH-dependent pathway has an important role in the maturation of the EGFR in cumulus cells and that activation of EGFR-dependent signaling is sufficient to induce expansion.

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Section: Egf Induces Tyrosine Phosphorylation Of Egfr and Other Cumulsupporting

confidence: 50%

Epidermal Growth Factor-Receptor Tyrosine Kinase Activity Regulates Expansion of Porcine Oocyte-Cumulus Cell Complexes In Vitro1

Procházka

Kaláb

Nagyová

2003

Biology of Reproduction

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“…Thus, the chromosomal location of EGF, its specific biochemi-cal actions including cell proliferation (Haining et al, 1991) and initiation of DNA synthesis (Tomooka et al, 1986), its ability to improve the embryonic development in vitro (Wood and Kaye, 1989;Paria and Dey, 1990), and its increased luminal content on day 12 of pregnancy followed by a decline to day 16 (Diehl et al, 1994) indicate that the EGF/EGFR system may play a significant role in embryonic and maternal interactions (Wollenhaupt et al, 1999). This is further supported by the finding that endometrial and conceptus tissues express EGFR (Zhang et al, 1992a(Zhang et al, , 1992bKennedy et al, 1994).…”

confidence: 60%

Mapping and microsatellite marker development for the porcine leukemia inhibitory factor receptor (LIFR) and epidermal growth factor receptor (EGFR) genes

Spötter¹,

Drögemüller²,

Kuiper³

et al. 2002

Cytogenet Genome Res

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Leukemia inhibitory factor receptor (LIFR), epidermal growth factor receptor (EGFR), and their respective ligands have been implicated in regulating growth and development of the early pig conceptus. We isolated a PAC clone containing the porcine gene for LIFR and a BAC clone with the porcine EGFR gene, respectively. On each of these clones one microsatellite marker was identified by sequencing a collection of subclones. These gene-associated markers were evaluated by genotyping of 202 unrelated boars of four different breeds. Based on fluorescence in situ hybridization and radiation hybrid mapping, the porcine LIFR gene was assigned to SSC16q13→q14. The EGFR gene mapped to SSC9q26.

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“…This is exemplified by the cross-linking of [ 25 I]-EGF to a 170-kDa membrane protein that has a molecular mass identical to that of the EGF-R in the mouse and other mammalian species [15,28,34,51,52], by the ability of EGF to stimulate phosphorylation of a synthetic exogenous substrate, and by the hybridization of a mouse EGF-R cRNA probe to three transcripts that are similar to those FIG. 7.…”

Section: Discussionmentioning

confidence: 99%

Expression of Epidermal Growth Factor Receptor in the Preimplantation Uterus and Blastocyst of the Western Spotted Skunk1

Paria

Das²,

Mead³

et al. 1994

Biology of Reproduction

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The western spotted skunk is unique in that its blastocysts undergo a 180-220-day period of arrested development before implantation. We investigated the potential role of epidermal growth factor (EGF)-related growth factors in regulating uterine and embryonic development in this species by studying the status of EGF receptor (EGF-R) in these tissues during delayed implantation and resumption of embryonic development. The cell-specific distribution of EGF binding sites and the expression of EGF-R mRNA were assayed by autoradiography and Northern blot analysis, respectively. The size of EGF-R was determined by affinity cross-linking studies, and its bioactivity was examined by determining EGF-dependent subcellular protein tyrosine kinase (PTK) activity. EGF binding sites were localized in the uterine luminal and glandular epithelium, endometrial stroma, myometrium, and blood vessels during both stages of pregnancy. As examined by Northern blot hybridization, a cRNA probe specific to mouse EGF-R hybridized to poly(A)+ RNA of skunk uteri. Transcripts similar to those of mouse uterine EGF-R were identified. [125I]-EGF was cross-linked to a 170-kDa protein both in the uterus and in blastocysts collected during the delayed implantation and periimplantation periods. However, EGF-induced PTK activity was significantly elevated above background levels during the period of renewed embryonic development, but not during arrested embryonic development. The results suggest that EGF-related growth factors may play an important role in regulating embryonic development in this species and that a change in the number and/or functional status of the EGF-R may be a prerequisite for blastocyst activation and implantation in the spotted skunk.

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Characterization of the epidermal growth factor receptor in preimplantation pig conceptuses

Cited by 19 publications

References 35 publications

Epidermal Growth Factor-Receptor Tyrosine Kinase Activity Regulates Expansion of Porcine Oocyte-Cumulus Cell Complexes In Vitro1

Epidermal Growth Factor-Receptor Tyrosine Kinase Activity Regulates Expansion of Porcine Oocyte-Cumulus Cell Complexes In Vitro1

Mapping and microsatellite marker development for the porcine leukemia inhibitory factor receptor (LIFR) and epidermal growth factor receptor (EGFR) genes

Expression of Epidermal Growth Factor Receptor in the Preimplantation Uterus and Blastocyst of the Western Spotted Skunk1

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