Characterization of the Ers Regulon of
            <i>Enterococcus faecalis</i>

Riboulet-Bisson, Eliette; Sanguinetti, Maurizio; Budin-Verneuil, Aurélie; Auffray, Yanick; Hartke, Axel; Giard, Jean‐Christophe

doi:10.1128/iai.00161-08

Cited by 28 publications

(38 citation statements)

References 45 publications

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“…The samples were analyzed with the CEQ8800 sequencing apparatus (Beckman Coulter). The determination of the DNA sequence of the protected region was carried out according to the method described by Riboulet-Bisson et al (2008).…”

Section: Methodsmentioning

confidence: 99%

Transcriptional factor Fur from Thermoplasma volcanium binds its own promoter DNA in a divalent cation-dependent manner

Ikeda

Minoshima

Satoh

et al. 2012

J. Gen. Appl. Microbiol.

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Because archaea possess many respiratory enzymes or radical scavengers with catalytic domains that contain iron, the expression of the genes encoding these enzymes might be regulated by iron acquisition. The genome of an archaeon, Thermoplasma volcanium contains a gene that encodes Fur (TVN0292). The fur gene of T. volcanium was amplifi ed by PCR, and cloned into plasmid pET28a. TvFur (T. volcanium Fur protein) was expressed in E. coli cells and then purifi ed. EMSA revealed that TvFur binds to its own promoter DNA. The binding to its own promoter was in an Mn 2+ -, Zn

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Section: Methodsmentioning

confidence: 99%

Transcriptional factor Fur from Thermoplasma volcanium binds its own promoter DNA in a divalent cation-dependent manner

Ikeda

Minoshima

Satoh

et al. 2012

J. Gen. Appl. Microbiol.

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“…Investigation of members of the Ers regulon showed that this reg-ulator directly interacts with the promoter regions of ers, ef0082, arcABC, and ef1459 (25). Sequence comparison of these regions allowed the characterization of a putative consensus sequence (AACATTTGTTG) that shows similarity to the PrfA box of Listeria (TTAACANNTGTTAA) (25).…”

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confidence: 99%

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confidence: 99%

“…Ers plays an important role in the survival of oxidative stress and in the survival of the bacterium in mouse macrophages, as well as in the virulence of E. faecalis (6,25). Investigation of members of the Ers regulon showed that this reg-ulator directly interacts with the promoter regions of ers, ef0082, arcABC, and ef1459 (25). Sequence comparison of these regions allowed the characterization of a putative consensus sequence (AACATTTGTTG) that shows similarity to the PrfA box of Listeria (TTAACANNTGTTAA) (25).…”

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confidence: 99%

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ace , Which Encodes an Adhesin in Enterococcus faecalis , Is Regulated by Ers and Is Involved in Virulence

Lebreton

Riboulet-Bisson

Serror³

et al. 2009

Infect Immun

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109

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Enterococcus faecalis is an opportunistic pathogen that causes numerous infectious diseases in humans and is a major agent of nosocomial infections. In this work, we showed that the recently identified transcriptional regulator Ers (PrfA like), known to be involved in the cellular metabolism and the virulence of E. faecalis, acts as a repressor of ace, which encodes a collagen-binding protein. We characterized the promoter region of ace, and transcriptional analysis by reverse transcription-quantitative PCR and mobility shift protein-DNA binding assays revealed that Ers directly regulates the expression of ace. Transcription of ace appeared to be induced by the presence of bile salts, probably via the deregulation of ers. Moreover, with an ace deletion mutant and the complemented strain and by using an insect (Galleria mellonella) virulence model, as well as in vivo-in vitro murine macrophage models, we demonstrated for the first time that Ace can be considered a virulence factor for E. faecalis. Furthermore, animal experiments revealed that Ace is also involved in urinary tract infection by E. faecalis.Enterococcus faecalis is a natural member of the intestinal microflora of warm-blooded animals and humans. However, E. faecalis remains an important opportunistic pathogen and represents one of the principal causes of nosocomial infections in the United States and Europe (33; for a review, see reference 20). Especially for immunocompromised patients, these infections include endocarditis, meningitis, pneumonia, peritonitis, visceral abscesses, urinary infections, and septicemia (13). About a dozen putative virulence factors have been identified in E. faecalis, but mechanisms of virulence remain not fully understood. These factors are involved in different steps of the infection process, such as attachment to host cells or extracellular matrix (ECM), macrophage resistance, tissue damage, and immune system evasion (20).For extracellular pathogens such as E. faecalis or Staphylococcus aureus, components of ECM or serum (i.e., collagen, fibronectin, and fibrinogen) are preferred targets for adhesion. During important steps of the infectious process, they interact with bacterial surface-exposed molecules, which include microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Ace, an adhesin that binds collagen (types I and IV) and laminin and belongs to the MSCRAMM family, was identified in E. faecalis by sequence homology with the virulence factor Cna, a well-characterized MSCRAMM in S. aureus (16,26). Synthesis of the A domain of Ace by S. aureus increased its arthritogenic potential to a level similar to that of S. aureus expressing Cna (34). Study of the sequence diversity of ace among several strains of E. faecalis has revealed an important variation in the number of repeated sequences (17). However, the role played by these regions has not yet been elucidated. The expression of Ace is significantly induced by high temperature (culture at 46°C) and in vivo by the presence of serum or ECM com...

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“…DNaseI Footprinting Analysis DNaseI footprinting analysis was performed according to the method described by Riboulet-Bisson et al 15) with some modification. The 5′ tvsod-ogg fragment was generated by PCR with pUC119-5′ tvsod-ogg as a template and a D4 dye end-labeled primer set of M13-20 (5′-D4-GTA AAA CGA CGG CCA GT-3′) and M13rev (5′-D4-GGA AAC AGC TAT GAC CAT G-3′).…”

Section: )mentioning

confidence: 99%

Specificity of Fur Binding to the Oxidative Stress Response Gene Promoter in the Facultative Anaerobic Archaeon <i>Thermoplasma volcanium</i>

Minoshima

Ikeda

Fujii

et al. 2014

Biological & Pharmaceutical Bulletin

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The genome of the facultative anaerobic thermoacidophilic archaeon Thermoplasma volcanium contains the open-reading frames (ORFs) tvsod and tvogg, which are predicted to encode a putative superoxide dismutase and an 8-oxoguanine DNA glycosylase, respectively. Tvsod is immediately upstream of tvogg, and these two ORFs are aligned in a head-to-tail manner in a single operon. A previous study showed that T. volcanium contains an ORF (TVN0292) encoding the ferric uptake regulator (Fur) and that the T. volcanium Fur protein (TvFur) binds to its own promoter in a metal-dependent manner in vitro. Here, we demonstrated that TvFur also binds to the tvsod-tvogg promoter and determined the TvFur-binding sequences in the tvsodtvogg promoter by DNaseI footprinting analysis. These results suggest that Fur is required for resistance against reactive oxygen species in this facultative anaerobic archaeon.

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Characterization of the Ers Regulon of Enterococcus faecalis

Cited by 28 publications

References 45 publications

Transcriptional factor Fur from Thermoplasma volcanium binds its own promoter DNA in a divalent cation-dependent manner

Transcriptional factor Fur from Thermoplasma volcanium binds its own promoter DNA in a divalent cation-dependent manner

ace , Which Encodes an Adhesin in Enterococcus faecalis , Is Regulated by Ers and Is Involved in Virulence

Specificity of Fur Binding to the Oxidative Stress Response Gene Promoter in the Facultative Anaerobic Archaeon <i>Thermoplasma volcanium</i>

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