Characterization of the estrogenic response to genistein in Japanese medaka (Oryzias latipes)

Zhang, Luling; Khan, Ikhlas A.; Foran, Christy M.

doi:10.1016/s1532-0456(02)00067-4

Cited by 38 publications

(42 citation statements)

References 26 publications

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“…In our previous study (Opalka et al, 2004) we observed a similar, inhibitory effect of genistein on basal and LH-stimulated testosterone secretion by cockerel testicular cells. In fish (Oryzias latipes) genistein injection resulted in decreased testosterone production from ex vivo testis and a comparable reduction in circulating testosterone (Zhang et al, 2002). Phytoestrogens affect ovarian and adrenal in vitro steroidogenesis in mammals.…”

Section: Discussionmentioning

confidence: 99%

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Opałka¹,

Kamińska²,

Piskuła

et al. 2006

British Poultry Science

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The aim of this study was to examine ganders at different stages of the reproductive season and the effect of: (1) diets with high phytoestrogen content and (2) in vitro phytoestrogen treatment on testosterone secretion by isolated Leydig cells. Thirty-six male Biłgoraj geese were fed control diets with low phytoestrogen content (containing grass meal) and diets with high phytoestrogens (containing alfalfa meal and soy). Testes were obtained from both groups of ganders at three different times of the breeding season: peak of reproductive activity (March), second half of reproductive activity (May) and beginning of photorefractoriness (July). Isolated Leydig cells were incubated with LH as well as genistein, daidzein, equol and coumestrol and the concentration of testosterone in the medium was determined by radioimmunoassay. The mean weight of testes from ganders in May and July decreased relative to their weights in March, but no significant differences among experimental groups were noted. No differences were observed in basal and LH-stimulated testosterone secretion by Leydig cells of ganders fed the control diets and the diets with higher phytoestrogen content. In July, LH did not stimulate testosterone secretion in either group. In vitro treatment with genistein, daidzein and equol (5 and 50 microM) inhibited basal and LH-stimulated testosterone production by Leydig cells from both groups. Coumestrol (5 and 50 microM) inhibited basal testosterone secretion only in March in the control group. Dietary exposure to phytoestrogens had a slight effect on in vitro testicular secretion in ganders. In vitro treatment with phytoestrogen inhibited testosterone production by Leydig cells. Genistein showed the strongest effect and coumestrol had the weakest influence on testicular secretion.

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Section: Discussionmentioning

confidence: 99%

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Opałka¹,

Kamińska²,

Piskuła

et al. 2006

British Poultry Science

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“…Zhang et al reported that genistein increased the estradiol production of ovaries and decreased testosterone production from ex vivo testis and a comparable reduction in circulating testosterone level in Japanese medaka (Zhang et al, 2002). The phytoestrogen beta-sitosterol was reported to alter the reproductive endocrine status of goldfish (MacLatchy and Van Der Kraak, 1995).…”

Section: Discussionmentioning

confidence: 99%

Xenopus laevis is a potential alternative model animal species to study reproductive toxicity of phytoestrogens

et al. 2006

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“…Intraperitoneal (ip) injections of genistein and equol to yearling Siberian sturgeon (Acipenser baeri) induced vitellogenesis, a physiological response associated with estrogenic activity (Pelissero et al 1991). In Japanese medaka (Oryzias latipes) exposed intraperitoneally to genistein, vitellogenin was not induced in males and females, but plasma 17β-estradiol (E 2 ) was increased in exposed females and plasma testosterone (T) levels were reduced in exposed males (Zhang et al 2002). Genistein-enriched diets were fed to rainbow trout (Oncorhynchus mykiss) for 1 year, and gametogenesis and reproductive efficiency of the trout were reduced (Bennetau- Pelissero et al 2001).…”

Section: Research | Articlementioning

confidence: 99%

Effects of the isoflavones genistein and equol on the gonadal development of Japanese medaka Oryzias latipes.

Kiparissis

Balch

Metcalfe

et al. 2003

Environ Health Perspect

122

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The estrogenic isoflavone compound genistein recently has been found in the effluents of sewage treatment plants and pulp mills, and the related compound equol has been detected in the runoff from agricultural fields treated with hog manure. Waterborne exposures of Japanese medaka (Oryzias latipes) to equol from soon after hatch to approximately 100 days posthatch induced gonadal intersex (i.e., testis-ova) in males at incidences of 10 and 87% in equol treatments of 0.4 and 0.8 µg/L, respectively. Exposure to the highest test concentration of genistein, 1,000 µg/L, also caused a low incidence (i.e., 12%) of gonadal intersex in male medaka. The ovaries of female medaka from both equol and genistein treatments showed delayed oocyte maturation, atretic oocytes, an enlarged ovarian lumen, proliferation of somatic stromal tissue, and primordial germ cells; responses were concentration dependent. Alterations to externally visible secondary sex characteristics occurred in medaka exposed to both equol and genistein. In treatments with 1,000 µg/L genistein, 72% of male medaka (as identified by the gonadal phenotype) showed feminized secondary sex characteristics. Gonadal intersex and alterations to secondary sex characteristics have been noted in several fish populations around the world. This laboratory study indicates that isoflavone compounds should be considered candidate estrogenic compounds that may be involved in the alteration of sexual development in feral fish populations.

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Characterization of the estrogenic response to genistein in Japanese medaka (Oryzias latipes)

Cited by 38 publications

References 26 publications

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Effects of phytoestrogens on testosterone secretion by Leydig cells from Biłgoraj ganders (Anser anser)

Xenopus laevis is a potential alternative model animal species to study reproductive toxicity of phytoestrogens

Effects of the isoflavones genistein and equol on the gonadal development of Japanese medaka Oryzias latipes.

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