Characterization of the Extended Myb-like DNA-binding Domain of Trithorax Group Protein Zeste

Mohrmann, Lisette; Kal, Arnoud J.; Verrijzer, C. Peter

doi:10.1074/jbc.m202341200

Cited by 17 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, both ends of this gene and some segments of the central region is highly conserved. However some parts of central regions vary (see screening of the alignment of orthologous genes in Figure S10) [56,57].…”

Section: Positively Selected Genes In M Oleifera Genomementioning

confidence: 99%

High quality reference genome of drumstick tree (Moringa oleifera Lam.), a potential perennial crop

Yang

Zeng

Zhang

et al. 2015

Sci. China Life Sci.

View full text Add to dashboard Cite

The drumstick tree (Moringa oleifera Lam.) is a perennial crop that has gained popularity in certain developing countries for its high-nutrition content and adaptability to arid and semi-arid environments. Here we report a high-quality draft genome sequence of M. oleifera. This assembly represents 91.78% of the estimated genome size and contains 19,465 protein-coding genes. Comparative genomic analysis between M. oleifera and related woody plant genomes helps clarify the general evolution of this species, while the identification of several species-specific gene families and positively selected genes in M. oleifera may help identify genes related to M. oleifera's high protein content, fast-growth, heat and stress tolerance. This reference genome greatly extends the basic research on M. oleifera, and may further promote applying genomics to enhanced breeding and improvement of M. oleifera. genome, drumstick tree, Moringa oleiferaCitation:

show abstract

Section: Positively Selected Genes In M Oleifera Genomementioning

confidence: 99%

High quality reference genome of drumstick tree (Moringa oleifera Lam.), a potential perennial crop

Yang

Zeng

Zhang

et al. 2015

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

“…Hence, profilin markedly alters the transcriptional activity of p42 POP and this effect depends strongly on an intact polyproline-binding site. Marocco et al, 1989) and the DBD contains three insertions, again similar to that found in Zeste (Mohrmann et al, 2002). Yet these modifications obviously do not interfere with the activity of p42 POP as a transcription factor.…”

Section: Fig 9 Profilin Modulates P42mentioning

confidence: 81%

“…Computer-assisted analysis of p42 POP suggested that p42 POP was indeed related to transcription factors of the Myb family, because it displayed a tryptophan cluster at its N-terminus and, indeed, binds to the consensus DNA recognition motif indicative of Myb proteins (PyAACG/TG) (Biedenkapp et al, 1988). However, the DBD of p42 POP includes only a single tryptophan cluster motif, similar to several Myb-related proteins identified in plants (Baranowskij et al, 1994;Kirik and Baumlein, 1996) and in the Zeste protein found in Drosophila (Mohrmann et al, 2002), whereas all mammalian members of the Myb transcription factor family known so far display three of these repeats. Furthermore, within the tryptophan cluster, the first tryptophan is replaced by a phenylalanine, a substitution described for several other Myb proteins (Kirik and Baumlein, 1996; Journal of Cell Science 118 (2) POP causes a decrease in transcription to less than 15% of the control value.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Profilin regulates the activity of p42POP, a novel Myb-related transcription factor

Lederer

Jockusch

Rothkegel

2005

Journal of Cell Science

View full text Add to dashboard Cite

Profilins, regulators of cytoplasmic actin dynamics, also bind to several nuclear proteins but the significance of these interactions is mostly unclear. Here, we describe a novel Myb-related transcription factor, p42POP, as a new ligand for profilin and show that profilin regulates its activity. p42POP comprises a unique combination of domains and is widely expressed in mouse tissues. In contrast to many other Myb proteins, it contains only one functional tryptophan-cluster motif. This is followed by an acidic domain, a leucine zipper that mediates dimerization and functional nuclear import and export signals that can direct p42POP to either the nuclear or the cytoplasmic compartment. Binding to profilins is mediated by a proline-rich cluster. p42POP-profilin complexes can be precipitated from cell lysates. In transfected cells displaying p42POP in the nucleus, nuclear profilin is markedly increased. When p42POP is anchored at mitochondrial membranes, profilin is targeted to this location. Hence, in a cellular environment, p42POP and profilin are found in the same protein complex. In luciferase assays, p42POP acts as repressor and this activity is substantially reduced by profilins, indicating that profilin can regulate p42POP activity and is therefore involved in gene regulation.

show abstract

“…How Su(z)2 1 and Psc 1 suppress z 1 is unclear, although much has been learned about Zeste, which is found in PRC1 Mulholland et al 2003) and is known to bind DNA (reviewed by Pirrotta 1991; also see Mohrmann et al 2002), self-associate or aggregate (reviewed by Pirrotta 1991; also see Chen and Pirrotta 1993;Rosen et al 1998), and participate in both gene activation and gene repression (Biggin et al 1988;Laney and Biggin 1992;Kal et al 2000;Hur et al 2002;Mulholland et al 2003;Dejardin and Cavalli 2004). Of the mechanisms being considered, several suggest that Su(z)2 and Psc interact with Zeste directly or within the context of a larger complex, the form or occurrence of such interactions being contingent on the mutant or wild-type state of the proteins (Mansukhani et al 1988;Wu and Goldberg 1989;Chen and Pirrotta 1993;Rastelli et al 1993;Rosen et al 1998;Saurin et al 2001;Mulholland et al 2003 1 .…”

mentioning

confidence: 99%

Molecular Genetic Analysis of Suppressor 2 of zeste Identifies Key Functional Domains

et al. 2009

View full text Add to dashboard Cite

The Su(z)2 complex contains Posterior sex combs (Psc) and Suppressor 2 of zeste [Su(z)2], two paralogous genes that likely arose by gene duplication. Psc encodes a Polycomb group protein that functions as a central component of the PRC1 complex, which maintains transcriptional repression of a wide array of genes. Although much is known about Psc, very little is known about Su(z)2, the analysis of which has been hampered by a dearth of alleles. We have generated new alleles of Su(z)2 and analyzed them at the genetic and molecular levels. Some of these alleles display negative complementation in that they cause lethality when heterozygous with the gain-of-function Su(z)2 1 allele but are hemizygous and, in some cases, homozygous viable. Interestingly, alleles of this class identify protein domains within Su(z)2 that are highly conserved in Psc and the mammalian Bmi-1 and Mel-18 proteins. We also find several domains of intrinsic disorder in the C-terminal regions of both Psc and Su(z)2 and suggest that these domains may contribute to the essential functions of both proteins.

show abstract

Characterization of the Extended Myb-like DNA-binding Domain of Trithorax Group Protein Zeste

Abstract: Zeste is a

Cited by 17 publications

References 52 publications

High quality reference genome of drumstick tree (Moringa oleifera Lam.), a potential perennial crop

High quality reference genome of drumstick tree (Moringa oleifera Lam.), a potential perennial crop

Profilin regulates the activity of p42POP, a novel Myb-related transcription factor

Molecular Genetic Analysis of Suppressor 2 of zeste Identifies Key Functional Domains

Contact Info

Product

Resources

About