Characterization of the extracellular polysaccharide of Porphyridium sp.: molecular weight determination and rheological properties

Geresh, Shimona; Adin, Itay; Yarmolinsky, E; Karpasas, Mark

doi:10.1016/s0144-8617(02)00019-x

Cited by 105 publications

(68 citation statements)

References 21 publications

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“…Size exclusion chromatography (SEC) is presently the most popular method for fractionation and characterization of both synthetic and natural polymers. [8][9][10][11] In our laboratory, a preparative SEC column packed with regenerated cellulose gel particles has been used successfully for fractionation of a dextran in water 12) and -D-glucan PC3 from Poria cocos sclerotium in dimethylsulfoxide (DMSO).…”

mentioning

confidence: 99%

Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide fromPoria cocos

Zhang¹,

Chen²,

Xu³

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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mentioning

confidence: 99%

Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide fromPoria cocos

Zhang¹,

Chen²,

Xu³

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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“…has been found to be 2.3×10 6 g/mol. Increased sonication of aqueous solution of the polysaccharide resulted in significantly lower viscosity, indicated by transition from weak gel-state to liquid-like state (Geresh et al, 2002).…”

Section: Characteristics Of Sulfated Polysaccharidesmentioning

confidence: 99%

Pharmacological, pharmaceutical, cosmetic and diagnostic applications of sulfated polysaccharides from marine algae and bacteria

Majee¹,

Avlani²,

Biswas³

2017

Afr. J. Pharm. Pharmacol.

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Marine environment with rich biodiversity offer unlimited choice for novel biopolymers. Sulfated polysaccharides isolated from marine algae and bacteria constitute an important group in the marinederived biomolecules and biopolymers. They possess unique structural features which can be exploited to their fullest potential in the development of new therapeutic molecules, design of nanocarriers and stimuli-responsive drug delivery systems, development of anti-aging and moisturizing creams and as molecular probes in diagnosis of cancers and cardiovascular diseases. The aim of the present review is to highlight the sources, characteristics and applications of sulfated polysaccharides and exopolysaccharides isolated from marine algae, cyanobacteria, extremophilic and halophilic bacteria. Detailed description of physicochemical properties and versatile applications of ulvan, fucoidan, galactofucan sulfate, laminarin, mauran, cyanobacterial exopolysaccharides and other lesser known exopolysaccharides of marine bacterial origin has been provided. In a nutshell, it can be concluded that sustainable exploitation of the renewable, diverse library of these unique and novel sulfated polysaccharides will unravel newer possibilities in future and will enrich the existing arsenal of biopolymers.

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“…Various approaches can be used to determine the mass of EPSs. Gel permeation chromatography (GPC) using multiangle laser light scattering (MALLS) detector coupled with RI detector in the HPLC system is widely used method to determine the molecular mass of EPSs (Geresh et al, 2002). Elution of EPS on GPC with a specific column (Sepharose CL 4B or Sephacryl-S 400) which was precalibrated T-series standard dextrans or pullulans (Cho et al, 2006) (known molecular weight) is an alternative method of determination of EPS molecular weight .The molecular weight of EPSs can also be determined by using the relative viscosity (´r) of EPSs by following formula (Brigand & Denis, 1990).…”

Section: Molecular Mass Determination and Purification Of Epssmentioning

confidence: 99%

Recent Trends in the Characterization of Microbial Exopolysaccharides

Madhuri¹,

Prabhakar²

2014

Orient. J. Chem.

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Bacterial polysaccharides that are secreted into the environment are termed as exopolysaccharides (EPSs). Depending on the monosaccharide composition, EPSs can be classified into homo (HoPSs) and heteropolysaccharides (HePSs). HoPSs consist of only one type of monosaccharide, mostly glucose or fructose. In the recent years, EPSs have drawn increasing attention of the researchers worldwide, not only for their thickening, texturizing and viscosifying properties, but also for their health promoting applications. These EPSs constitute an alternative class of biothickeners, which have also been proved to have good emulsifying property, apart from their texture promoting ability in various foods. The biochemical properties of the EPSs depend on the primary structure of the EPSs. Hence it is very important to know the monomeric composition of the EPSs and their gycosidic bonds. HPLC, GC, GC-MS, NMR, Capillary electrophoresis and various bioinformatics tools can be used to determine the chemical characteristics of the EPSs.

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Characterization of the extracellular polysaccharide of Porphyridium sp.: molecular weight determination and rheological properties

Cited by 105 publications

References 21 publications

Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide fromPoria cocos

Effect of Molecular Mass on Antitumor Activity of Heteropolysaccharide fromPoria cocos

Pharmacological, pharmaceutical, cosmetic and diagnostic applications of sulfated polysaccharides from marine algae and bacteria

Recent Trends in the Characterization of Microbial Exopolysaccharides

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