Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph,<i>Pseudoalteromonas</i>(BB2-AT2): multiple bacterioferritin copies enable significant Fe storage

Mazzotta, Michael G.; McIlvin, Matthew R.; Saito, Mak A.

doi:10.1039/d0mt00034e

Cited by 21 publications

(19 citation statements)

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“…In view of preponderant strains of Vibrio , Pseudoalteromonas , and Marinobacterium for 30 days, it was found that nutrients from Saharan dust in marine surface waters promoted the formation of Vibrio bloom, and dust-associated iron was a vital driving factor of Vibrio population dynamics ( Westrich et al, 2016 ). Besides, iron was extremely scarce in the marine, but Pseudoalteromonas with broad iron storage capacity can produce a large number of TonB iron complex transporters for obtaining iron ( Mazzotta et al, 2020 ). Marinobacterium species were commonly identified and were associated with metal corrosion processes, mainly in offshore oil structures ( Cluff et al, 2014 ; Brauer et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Effect of Photoreduction of Semiconducting Iron Mineral—Goethite on Microbial Community in the Marine Euphotic Zone

Liu

Ding

et al. 2022

Front. Microbiol.

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Marine euphotic zone is the pivotal region for interplay of light-mineral–microorganism and elements cycle, in which semiconducting minerals exist widely and iron-bearing goethite is a typical and widespread one. In this work, we have conducted in-depth researches on the effect of ferrous [Fe(II)] ions dissolved by photoreduction of goethite on microbial community structure and diversity. The mineral phase, structure and morphology of synthesized goethite were characterized by Raman, X-ray diffraction (XRD), energy disperse spectroscopy (EDS), environmental scanning electron microscope (ESEM), and atomic force microscope (AFM). Photoelectrochemical measurements tested photoelectric response and redox activity of goethite, having proved its significant property of photoelectric response with 44.11% increment of the average photocurrent density relative to the dark current density. The photoreduction experiments of goethite were conducted under light condition in simulated seawater. It has suggested the photoreduction of goethite could occur and Fe(III) was reduced to Fe(II). The dissolved Fe(II) from the photoreduction of goethite under light condition was nearly 11 times than that group without light after a 10-day reaction. Furthermore, results of microbial community sequencing analysis indicated that dissolved Fe(II) could affect the structure and regulate the decrease of microbial community diversity. The emergence of dominant bacteria associated with iron oxidation and transport protein has suggested their obvious selectivity and adaptability in the environment with adding dissolved Fe(II). This work revealed the photoreduction process of semiconducting goethite was remarkable, giving rise to a non-negligible dissolved Fe(II) and its selective effect on the structure, diversity, as well as the function of microbial community. This light-induced interaction between minerals and microorganisms may also further regulate correlative metabolic pathways of carbon cycle in the marine euphotic zone.

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Section: Resultsmentioning

confidence: 99%

Effect of Photoreduction of Semiconducting Iron Mineral—Goethite on Microbial Community in the Marine Euphotic Zone

Liu

Ding

et al. 2022

Front. Microbiol.

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“…The degree of competition between bacteria-phytoplankton is very sensitive to the modeled FL bacterial Fe demand and quotas, which are still poorly constrained at between 1e − 6 and more than 80e − 6 mol Fe/mol C by observations (Fourquez et al, 2015;Mazzotta et al, 2020;Tortell et al, 1996Tortell et al, , 1999). An elevated FL bacterial demand for Fe, either to meet their metabolic requirements or to build a large Fe internal storage through luxury uptake, enhances their competition with phytoplankton (Bowie et al, 2001;Ratnarajah et al, 2021) and the amount of Fe that is cycled through FL bacteria (Figure 12).…”

Section: Discussionmentioning

confidence: 99%

“…as described in details in Equation 19in Aumont et al (2015). The modeled FL bacterial Fe quota in PISCES varies between the minimum quota and a maximum Fe quota 𝐴𝐴 𝐴𝐴 𝐹𝐹 𝐹𝐹𝐹𝐹𝐹 max , which is set to 80e − 6 mol Fe/mol C (Mazzotta et al, 2020).…”

Section: Iron Quota In Fl Bacteriamentioning

confidence: 99%

“…As mentioned in the previous sections, these demands are poorly constrained due to the paucity of in situ and laboratory observations. The few available observations suggest a wide range of values for the parameters used in our model, especially for the minimum, optimal, and maximum FL bacterial Fe quotas (Fourquez et al, 2015(Fourquez et al, , 2020Mazzotta et al, 2020;Tortell et al, 1999) Using results of this sensitivity analysis, we then discuss consequences of the resource competition between FL bacteria and phytoplankton on phytoplankton blooms and carbon export.…”

Section: Sensitivities Of Fl Bacterial Growth and Activities To Their...mentioning

confidence: 99%

“…For instance, it is still unclear if Fe is the ultimate factor limiting the growth of FL bacteria in the ocean, especially when both labile DOC and Fe concentrations are low (Church et al, 2000;Obernosterer et al, 2015;Ratnarajah et al, 2021). In addition, the limited existing ocean measurements have reported a wide range of values for the Fe contents of FL bacteria, resulting in a large uncertainty in our knowledge of the FL bacterial Fe demand, storage, and uptake capability (Fourquez et al, 2015;Mazzotta et al, 2020;Tortell et al, 1999). In this context, the ocean biogeochemistry models that integrate marine ecosystem dynamics into the global ocean circulation can be useful tools to fill these knowledge gaps.…”

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confidence: 99%

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Examining the Interaction Between Free‐Living Bacteria and Iron in the Global Ocean

Pham

Aumont

Ratnarajah

et al. 2022

Global Biogeochemical Cycles

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Heterotrophic bacteria (hereafter bacteria) account for a significant portion of the ocean carbon biomass (Buitenhuis et al., 2012) and play various roles in oceanic nutrient cycles. Despite the wide variety of bacteria that live under various conditions (Sogin et al., 2006;Zakem et al., 2020), free-living (FL), carbon-oxidizing bacteria have been the focus of previous studies due to their control on the flow of carbon and bio-essential elements through the marine food web (Azam & Malfatti, 2007;del Giorgio & Duarte, 2002;Jiao et al., 2010). Carbon-oxidizing bacteria either transforms a fraction of the organic matter fixed by phytoplankton into a highly refractory form, thus keeping carbon away from the atmosphere for several millennia or respires the fixed organic matter to release inorganic carbon and nutrients back to the seawater, thus sustaining oceanic primary production (Jiao et al., 2010(Jiao et al., , 2014. Other bacterial groups, such as particle-attached, nitrogen-oxidizing, and sulfur-oxidizing bacteria and archaea, carry out various chemical transformations, which modulate the ocean nitrogen and sulfur cycles and produce potent greenhouse gas (nitrous oxide and methane) (

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Homeostasis drives intense microbial trace metal processing on marine particles

Debeljak

Blain

Merwe

et al. 2021

Limnology & Oceanography

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As marine microorganisms and environmental conditions coevolved over geological timescales, metals have been incorporated into all essential metabolic processes. In the modern ocean, metals are present from trace amounts limiting microbial growth to toxic concentrations. Dissolved trace metals are a major bioavailable reservoir. However, the acquisition of metals from marine particles remains largely unexplored. Here, we combined chemical characterization and a comparative metatranscriptomics approach to investigate the availability of nine metals of biological importance on particles collected in the region of Heard Island (Indian sector of the Southern Ocean). Elemental ratios identified particulate matter as a potential source of metals for prokaryotes. The expression of genes for the uptake of metals through various mechanisms demonstrated that particles are a bioavailable reservoir. But genes involved in the control of resistance to metal toxicity, storage, sensing, and regulation were also highly expressed. Our observations suggest that homeostasis associated with a diverse prokaryotic community is the overarching mechanism that enhances the trace element processing on particles. These results provide clues that microbial activity on particles is critical in the redistribution of trace elements between different fractions and chemical forms in the ocean.

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Characterization of the Fe metalloproteome of a ubiquitous marine heterotroph,Pseudoalteromonas(BB2-AT2): multiple bacterioferritin copies enable significant Fe storage

Abstract: Despite the extreme scarcity of Fe in seawater, the marine heterotroph Pseudoalteromonas has expansive Fe storage capacity and utilization strategies.

Cited by 21 publications

References 64 publications

Effect of Photoreduction of Semiconducting Iron Mineral—Goethite on Microbial Community in the Marine Euphotic Zone

Effect of Photoreduction of Semiconducting Iron Mineral—Goethite on Microbial Community in the Marine Euphotic Zone

Examining the Interaction Between Free‐Living Bacteria and Iron in the Global Ocean

Homeostasis drives intense microbial trace metal processing on marine particles

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