Characterization of the gag/fusion protein encoded by the defective Duplan retrovirus inducing murine acquired immunodeficiency syndrome

Huang, Ming; Jolicoeur, Paul

doi:10.1128/jvi.64.12.5764-5772.1990

Cited by 46 publications

(36 citation statements)

References 45 publications

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“…Each antiserum was checked by immunoprecipitation and found to react specifically with Gag precursors of MuLV and with individual Gag proteins labeled with [ 35 S]methionine or [ 3 H]leucine (data not shown). Rabbit R2 antiserum specific for the MAIDS-defective virus p12 gag region was described previously (6).…”

Section: Methodsmentioning

confidence: 99%

“…In contrast to the Gag precursor Pr65 gag from helper MuLVs, the Pr60 gag from the MAIDS-defective virus does not appear to be cleaved (6). In fibroblasts, Pr60 gag has been found to be myristylated, attached to the plasma membrane, and phosphorylated (6).…”

mentioning

confidence: 87%

“…This Pr60 gag protein has been detected both in fibroblasts harboring the defective viral genome (3,6) and in lymphoid tissues of mice inoculated with the virus (8). In contrast to the Gag precursor Pr65 gag from helper MuLVs, the Pr60 gag from the MAIDS-defective virus does not appear to be cleaved (6). In fibroblasts, Pr60 gag has been found to be myristylated, attached to the plasma membrane, and phosphorylated (6).…”

mentioning

confidence: 98%

“…Another short ORF is present at the 3Ј end of the genome (nucleotides [nt] 3241 to 3975) in sequences from remnants of the pol and env genes. So far, the only detected protein encoded by this virus is a 60-kDa Gag precursor (Pr60 gag ) protein (3,6). This Pr60 gag protein has been detected both in fibroblasts harboring the defective viral genome (3,6) and in lymphoid tissues of mice inoculated with the virus (8).…”

mentioning

confidence: 99%

“…So far, the only detected protein encoded by this virus is a 60-kDa Gag precursor (Pr60 gag ) protein (3,6). This Pr60 gag protein has been detected both in fibroblasts harboring the defective viral genome (3,6) and in lymphoid tissues of mice inoculated with the virus (8). In contrast to the Gag precursor Pr65 gag from helper MuLVs, the Pr60 gag from the MAIDS-defective virus does not appear to be cleaved (6).…”

mentioning

confidence: 99%

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Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease

Huang

Hanna

Jolicoeur

1995

J Virol

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Pr60 gag appears to be the only protein encoded by the murine AIDS (MAIDS)-defective virus. To study the role of Pr60 gag or some other sequences of the viral genome in the pathogenicity of the virus, we have generated mutants of the defective viral genome. These mutant defective viruses, prepared as helper-free stocks, were inoculated into susceptible C57BL/6 mice. Mutant Du5H-A virus, which had a stop codon within gag MA(p15), did not induce target cell proliferation or MAIDS. Mutants Du5H-B and -C encoded truncated Pr60 gag proteins containing, respectively, MA(p15)-p12 or MA(p15)-p12 and part of CA(p30). These mutants showed a very limited capacity to induce early cell expansion and were poorly pathogenic. Only recombinant (revertant) viruses were recovered from organs of diseased mice inoculated with these two mutants. Mutant Du5H-D was generated by deleting 1.4 kbp of the 3-end sequences, outside the gag coding region. The levels of RNA and proteins made by this mutant were low. This mutant also reverted frequently but was nevertheless able to induce MAIDS at a low efficiency without reverting. Our results indicate that the Pr60 gag protein is necessary and sufficient to induce MAIDS. These data also suggest that the Pr60 gag protein needs to be relatively intact to be fully pathogenic. In addition, our study shows a very high reversion rate of some mutants and emphasizes the need to check for the presence of revertant (recombinant) viruses in diseased organs when working with mutants of the MAIDS-defective virus.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 87%

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease

Huang

Hanna

Jolicoeur

1995

J Virol

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Apoptotic death of lymphocytes in murine acquired immunodeficiency syndrome: Involvement of Fas‐Fas ligand interaction

et al. 1995

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Murine acquired immunodeficiency syndrome (MAIDS) is caused by a defective murine leukemia virus. The disease is characterized by abnormal lymphoproliferation, impaired T and B cell function and aberrant regulation of cytokines. Both T and B lymphocytes show activated phenotypes, but undergo apoptotic death with characteristic DNA fragmentation. These results indicate the presence of a continuous activation death pathway of the lymphocytes in MAIDS. Overexpression of the bcl-2 transgene in lymphocytes showed no effect on the apoptotic cell death or on the development of the disease. In contrast, mice carrying mutations in either Fas or Fas ligand exhibited accelerated progression of the disease upon infection with MAIDS virus. These results suggest the involvement of Fas-Fas ligand system in the pathogenesis of MAIDS.

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Induction of Endogenous Mammary Tumor Virus in Lymphocytes Infected with Murine Acquired Immunodeficiency Syndrome Virus

Gayama

Doyon

Vaupel

et al. 1998

Cellular Immunology

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Characterization of the gag/fusion protein encoded by the defective Duplan retrovirus inducing murine acquired immunodeficiency syndrome

Abstract: Murine acquired immunodeficiency syndrome is induced by a defective retrovirus. Sequencing of this defective viral genome revealed a long open reading frame which encodes a putative gag/fusion protein, N-MA-p12-CA-NC-COOH, (D.

Cited by 46 publications

References 45 publications

Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease

Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease

Apoptotic death of lymphocytes in murine acquired immunodeficiency syndrome: Involvement of Fas‐Fas ligand interaction

Induction of Endogenous Mammary Tumor Virus in Lymphocytes Infected with Murine Acquired Immunodeficiency Syndrome Virus

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