Characterization of the genetic structure of mcr-1 gene among Escherichia coli isolates recovered from surface waters and sediments from Ecuador

Calero-Cáceres, William; Tadesse, Daniel A.; Jaramillo, Katherine; Villavicencio, Xavier; Mero, Efraín; Lalaleo, Liliana; Welsh, Caitlin; Villacís, José E; Quentin, Emmanuelle; Parra, Henry; Ramírez, María Soledad; Harries, Anthony; Balcázar, José Luís

doi:10.1016/j.scitotenv.2021.150566

Cited by 12 publications

(11 citation statements)

References 33 publications

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“…It emphasizes the urgent need to implement and improve sewage treatment processes, along with stricter environmental monitoring, to prevent the spread of potentially pathogenic bacteria and to curb the escalation of AMR [ 32 ]. This is particularly crucial in countries like Ecuador, which have a low rate of wastewater treatment coverage [ 13 ]. By addressing these environmental factors, we can take a significant step towards mitigating the risks associated with AMR dissemination, ultimately protecting public health and preserving the effectiveness of current antibiotics [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…The discharge of untreated or partially treated wastewater can have significant impacts on the quality of water bodies, including increased levels of pollutants, bacteria, and other pathogens that can pose a risk to public health and the environment [ 12 ]. In Ecuador, recent studies indicated high prevalence of AMR bacteria in surface waters impacted by anthropogenic activities, including irrigation channels [ 13 ]. In this study, we analyzed sediment samples obtained from an irrigation channel used for agricultural purposes in the Province of Pichincha in Ecuador using microbiological techniques and whole genome sequencing (WGS).…”

Section: Introductionmentioning

confidence: 99%

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Genomic characterization of a WHO critical priority isolate Enterobacter kobei ST2070 harboring OXA-10, KPC-2, and CTX-M-12 recovered from a water irrigation channel in Ecuador

Corrales-Martínez,

Jaramillo,

Tadesse

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic characterization of a WHO critical priority isolate Enterobacter kobei ST2070 harboring OXA-10, KPC-2, and CTX-M-12 recovered from a water irrigation channel in Ecuador

Corrales-Martínez,

Jaramillo,

Tadesse

et al. 2024

Heliyon

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“…The WOAH report also showed that colistin was the antibiotic mostly commonly used for growth promotion. There are several reports in Ecuador and Latin America about dissemination and high levels of colistin resistance in human, animals, and the environment ( 25 , 26 ). Consequently, in 2019 Agrocalidad issued a ban on the use of this antibiotic in animals in Ecuador ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

High levels of antimicrobial resistance in Escherichia coli and Salmonella from poultry in Ecuador

Amancha¹,

Celis²,

Irazabal³

et al. 2023

Revista Panamericana De Salud Pública

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Objective. To describe antimicrobial resistance profiles of Escherichia coli and Salmonella spp. isolated from chicken carcasses and the antimicrobials commonly used in animals in Ecuador and provide information on antimicrobial resistance patterns for implementing evidence-based corrective measures. Methods. Meat samples were collected from chicken carcasses in 199 slaughterhouses across Ecuador as part of a national pilot study for monitoring antimicrobial resistance in agricultural sources in 2019. Samples were tested for E. coli and Salmonella spp. Sensitivity to 10 critically important and three highly important antimicrobials (from a human health perspective) was assessed. The country report submitted to the World Organization for Animal Health was accessed to extract the quantity of antimicrobials produced or imported for use in animals. Results. Of 383 samples, E. coli was isolated from 148 (39%) and Salmonella spp. from 20 (5%) samples. Ninety percent of the isolates were resistant to at least one critically important antimicrobial. Resistance was highest to erythromycin (E. coli 76%; Salmonella spp. 85%) and tetracycline (E. coli 71%; Salmonella spp. 90%). Critically or highly important antimicrobials (colistin, tetracycline, trimethoprim/sulfamethoxazole) formed the bulk (87%) of antimicrobials used in animals as per the World Organization for Animal Health report. Conclusions. High prevalence of antimicrobial resistance in poultry in Ecuador calls for the development of guidelines and regulations on the use of antimicrobials and for engagement with livestock producers. The existing surveillance system needs to be strengthened to improve the monitoring of antimicrobial use and evolving resistance patterns.

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“…On the contrary, the data on mcr location in E. coli isolated from humans and animals in Ecuador is limited. In a study on characteristics of the mcr gene among E. coli from waters and sediments in Ecuador, only 1 of 459 Enterobacteriaceae bacteria carried chromosomal mcr [36].…”

Section: Location and Structure Of Mcr-transposonmentioning

confidence: 99%

Comparative genome analysis of colistin-resistant Escherichia coli harboring mcr isolated from rural community residents in Ecuador and Vietnam

Thanh Hoang,

Yamamoto,

Calvopina

et al. 2023

PLoS ONE

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The spread of colistin-resistant bacteria among rural community residents of low- and middle-income countries is a major threat to community health. Although the mechanism of the spread of colistin-resistant bacteria in communities is unknown, geographic and regional characteristics may influence it. To elucidate the spread mechanism of colistin-resistant bacteria, we analyzed the genomes of colistin-resistant Escherichia coli isolated from Vietnam and Ecuador residents, which are geographically and socially different. Stool specimens of 139 and 98 healthy residents from Ecuador and Vietnam rural communities, respectively, were analyzed for colistin-resistant E. coli with mcr. Its prevalence in the residents of all the communities assessed was high and approximately equal in both countries: 71.8% in Ecuador and 69.4% in Vietnam. A phylogenetic tree analysis revealed that the sequence type of colistin-resistant E. coli was diverse and the major sequence types were different between the two countries. The location of mcr in the isolates showed that the proportion of chromosomal mcr was 35.1% and 8.5% in the Vietnam and Ecuador isolates, respectively. Most of these chromosomal mcr genes (75%–76%) had an intact mcr-transposon Tn6330. Contrastingly, the replicon types of the mcr-carrying-plasmids were diverse in both countries, but almost all belonged to IncI2 in Ecuador and IncX1/X4 in Vietnam. Approximately 26%–45% of these mcr-plasmids had other resistance genes, which also varied between countries. These results suggest that although the overall profile of the colistin-resistant E. coli isolates is diverse in these countries, the phylogenesis of the isolates and mcr-carrying plasmids has regional characteristics. Although the contributing factors are not clear, it is obvious that the overall profile of colistin-resistant bacteria dissemination varies between countries. Such different epidemic patterns are important for establishing country-specific countermeasures against colistin-resistant bacteria.

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Characterization of the genetic structure of mcr-1 gene among Escherichia coli isolates recovered from surface waters and sediments from Ecuador

Cited by 12 publications

References 33 publications

Genomic characterization of a WHO critical priority isolate Enterobacter kobei ST2070 harboring OXA-10, KPC-2, and CTX-M-12 recovered from a water irrigation channel in Ecuador

Genomic characterization of a WHO critical priority isolate Enterobacter kobei ST2070 harboring OXA-10, KPC-2, and CTX-M-12 recovered from a water irrigation channel in Ecuador

High levels of antimicrobial resistance in Escherichia coli and Salmonella from poultry in Ecuador

Comparative genome analysis of colistin-resistant Escherichia coli harboring mcr isolated from rural community residents in Ecuador and Vietnam

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