Characterization of the Glycoproteins of Crimean-Congo Hemorrhagic Fever Virus

Sanchez, Angela J.; Vincent, Martin J.; Nichol, Stuart T.

doi:10.1128/jvi.76.14.7263-7275.2002

Cited by 182 publications

(237 citation statements)

References 38 publications

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“…CCHFV glycoproteins contain an exceptionally large number of cysteine residues, suggesting the presence of many disulfide bonds and a complex secondary structure. The N-terminus of G N has mucin-like features, with the potential for extensive O-glycosylation (Sanchez et al, 2002;Papa et al, 2002c;Bertolotti-Ciarlet et al, 2005). Interestingly, the C-terminal cytoplasmic tail of G N contains two zinc fingers, which can bind viral RNA (Estrada and De Guzman, 2011).…”

Section: The M Segmentmentioning

confidence: 99%

Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity

et al. 2013

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Bente, Dennis A.; Forrester, Naomi L.; Watts, Douglas M.; McAuley, Alexander J.; Whitehouse, Chris A.; and Bray, Mike, "CrimeanCongo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity" (2013 b s t r a c tCrimean-Congo hemorrhagic fever (CCHF) is the most important tick-borne viral disease of humans, causing sporadic cases or outbreaks of severe illness across a huge geographic area, from western China to the Middle East and southeastern Europe and throughout most of Africa. CCHFV is maintained in vertical and horizontal transmission cycles involving ixodid ticks and a variety of wild and domestic vertebrates, which do not show signs of illness. The virus circulates in a number of tick genera, but Hyalomma ticks are the principal source of human infection, probably because both immature and adult forms actively seek hosts for the blood meals required at each stage of maturation. CCHF occurs most frequently among agricultural workers following the bite of an infected tick, and to a lesser extent among slaughterhouse workers exposed to the blood and tissues of infected livestock and medical personnel through contact with the body fluids of infected patients. CCHFV is the most genetically diverse of the arboviruses, with nucleotide sequence differences among isolates ranging from 20% for the viral S segment to 31% for the M segment. Viruses with diverse sequences can be found within the same geographic area, while closely related viruses have been isolated in far distant regions, suggesting that widespread dispersion of CCHFV has occurred at times in the past, possibly by ticks carried on migratory birds or through the international livestock trade. Reassortment among genome segments during co-infection of ticks or vertebrates appears to have played an important role in generating diversity, and represents a potential future source of novel viruses. In this article, we first review current knowledge of CCHFV, summarizing its molecular biology, maintenance and transmission, epidemiology and geographic range. We also include an extensive discussion of CCHFV genetic diversity, including maps of the range of the virus with superimposed phylogenetic trees. We then review the features of CCHF, including the clinical syndrome, diagnosis, treatment, pathogenesis, vaccine development and laboratory animal models of CCHF. The paper ends with a discussion of the possible future geographic range of the virus. For the benefit of researchers, we include a Supplementary Table listing all published reports of CCHF cases and outbreaks in the English-language literature, plus some principal articles in other languages, with total case numbers, case fatality rates and all CCHFV strains on GenBank.

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Section: The M Segmentmentioning

confidence: 99%

Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity

et al. 2013

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“…Transfecting cells, radiolabeling, and immune precipitating the expression products revealed products of the expected sizes for mature Gn (37 kDa) and Gc (75 kDa) and a larger product, which might be the 140 kDa precursor of Gn [24] (Fig. 1B).…”

Section: Dna Vaccine For Cchfvmentioning

confidence: 99%

“…Unlike those of other viruses in the family, however, the M segment precursor of CCHFV Gn and Gc has been found to undergo at least two post-translational proteolytic cleavage events. A precursor of the Gn glycoprotein is cleaved at the conserved motifs RSKR and RRLL, potentially releasing a highly variable mucin-like domain at its amino terminus, and a second N-terminal domain of approximately 35 kDa (P35) as well as the 37 kDa mature Gn [24,25]. An 85 kDa precursor of Gc has also been described [24]; however, the cleavage events leading to the mature 75 kDa Gc protein have not been determined.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of combination DNA vaccines for Rift Valley fever virus, tick-borne encephalitis virus, Hantaan virus, and Crimean Congo hemorrhagic fever virus

Spik

Shurtleff

McElroy

et al. 2006

Vaccine

132

109

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DNA vaccines for Rift Valley fever virus (RVFV), Crimean Congo hemorrhagic fever virus (CCHFV), tick-borne encephalitis virus (TBEV), and Hantaan virus (HTNV), were tested in mice alone or in various combinations. The bunyavirus vaccines (RVFV, CCHFV, and HTNV) expressed Gn and Gc genes, and the flavivirus vaccine (TBEV) expressed the preM and E genes. All vaccines were delivered by gene gun. The TBEV DNA vaccine and the RVFV DNA vaccine elicited similar levels of antibodies and protected mice from challenge when delivered alone or in combination with other DNAs. Although in general, the HTNV and CCHFV DNA vaccines were not very immunogenic in mice, there were no major differences in performance when given alone or in combination with the other vaccines. Published by Elsevier Ltd.

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“…Further analysis is necessary to determine which of the potential N-gly sites are used in DUGV and Kupe virus proteins. DUGV and Kupe virus M segment ORFs contain a highly variable, mucin-like region near the amino terminus, as described for the genome of CCHFV (9,11). This ≈100-aa region in DUGV and Kupe virus is shorter than the 243-248-aa region identifi ed in CCHFV, but this region in both viruses contains similarly high amino acid sequence variability, increased frequency of serine, threonine, and proline residues, and more highly predicted O-linked glycosylation than for the rest of the ORF.…”

Section: Genome Analysismentioning

confidence: 99%

Kupe Virus, a New Virus in the FamilyBunyaviridae, GenusNairovirus, Kenya

Crabtree¹,

Sang²,

Miller³

2009

Emerg. Infect. Dis.

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We have previously described isolation and preliminary identifi cation of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV. We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

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Characterization of the Glycoproteins of Crimean-Congo Hemorrhagic Fever Virus

Abstract: Crimean-Congo hemorrhagic fever (CCHF) virus is

Cited by 182 publications

References 38 publications

Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity

Crimean-Congo hemorrhagic fever: History, epidemiology, pathogenesis, clinical syndrome and genetic diversity

Immunogenicity of combination DNA vaccines for Rift Valley fever virus, tick-borne encephalitis virus, Hantaan virus, and Crimean Congo hemorrhagic fever virus

Kupe Virus, a New Virus in the FamilyBunyaviridae, GenusNairovirus, Kenya

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