Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

Peñaloza, Enrique; Santiago, Margarita; Cabrera, Sergio; Muñoz, Gastón; Corcuera, Luís J.; Silva, Herman

doi:10.1007/s10535-016-0672-9

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…This AtPHT1;4 promoter system can be used to improve the rhizosphere for maize and improve soil Pi uptake efficiency when maize plants experience Pi stress . A similar approach using the promoter of AtPHT1;4 in transgenic rice lines showed that high soil Pi content significantly inhibited the number of GUS transcripts …”

Section: Root Hair Development and Adaptation Under Abiotic Stressmentioning

confidence: 99%

“…224 A similar approach using the promoter of AtPHT1;4 in transgenic rice lines showed that high soil Pi content significantly inhibited the number of GUS transcripts. 225 Magnesium (Mg) Deficiency. Magnesium is an essential mineral element for chlorophyll synthesis and therefore plays a critical role in the aboveground parts of plants.…”

Section: Under Abiotic Stressmentioning

confidence: 99%

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Root Hair Development and Adaptation to Abiotic Stress

Zhang,

Yang,

Zhang

et al. 2023

J. Agric. Food Chem.

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Root hairs tie the root system to the soil substrate, facilitate water and nutrient absorption, and enable the interaction with microbes in the soil. Root hair development can be classified into three main development types (I−III). Root hair development type III has been extensively studied, mainly represented using the model plant Arabidopsis thaliana. Transcription factors, plant hormones, and proteins are involved at different root hair developmental stages. The mechanisms underlying development in types I and II have been examined using other representative plant species but have not been studied as intensively.Many key developmental genes in types I and II are highly homologous with those in type III, exhibiting conservation of related mechanisms. Root hairs are also involved in the regulation of plant response to abiotic stress by altering developmental patterns. Abiotic stress, regulatory genes, and plant hormones jointly regulate root hair development and growth; however, few studies have focused on how root hair recognizes abiotic stress signals. This review examines the molecular mechanisms of root hair development and adaptations under stress, and prospective future developments in root hair research are also discussed.

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Section: Root Hair Development and Adaptation Under Abiotic Stressmentioning

confidence: 99%

Section: Under Abiotic Stressmentioning

confidence: 99%

Root Hair Development and Adaptation to Abiotic Stress

Zhang,

Yang,

Zhang

et al. 2023

J. Agric. Food Chem.

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“…For plant root system, the processes of phosphorus absorption from environment and transfer of the phosphorus nutrient to tissues and cells are mediated by a phosphate transporter (PT) located in the plasma membrane (23). There have been several reports on phosphate transporter genes in wheat (24)(25)(26), but most studies on this field have aimed only at function of these genes and regulation of phosphate transporters by arbuscular mycorrhizal fungi in wheat (24,26,(27)(28)(29), and few studies have focused on regulatory effects of PGPR on these genes in wheat. TaPT4, one member of high-affinity phosphate transporter gene, was found to have strong affinity to phosphorus, and to play a significant role in the absorption of phosphorus under conditions of low phosphorus stress in wheat (26,(30)(31).…”

Section: Introductionmentioning

confidence: 99%

Phosphate-Solubilizing Pseudomonas sp. Strain P34-L Promotes Wheat Growth by Colonizing the Wheat Rhizosphere and Improving the Wheat Root System and Soil Phosphorus Nutritional Status

Liu

Jiang

et al. 2019

J Plant Growth Regul

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The ability to colonize the rhizosphere is an important basics requirement for field application of plant growth-promoting rhizobacteria (PGPR) strains. There are complex signal exchanges and mutual recognition between microbes and plants. In this study, phosphate-solubilizing Pseudomonas sp. P34, a PGPR strain with affinity to wheat, was isolated from the wheat rhizosphere by wheat germ agglutinin (WGA). The plasmid pTR102 harboring the luciferase luxAB gene was transferred into P34 to create P34-L. The labeled strain was used to track the temporal and spatial characteristics of colonization in wheat rhizosphere and its effects on wheat development. The transcript level of phosphate transporter gene TaPT4, a phosphorus deficiency indicator gene, in wheat roots was monitored by quantitative reverse-transcription PCR. The experimental results indicated that there was a high density of stain P34-L within the top 8-cm depth of the wheat rhizosphere on day 36 of wheat growth. The strain could survive in the wheat rhizosphere for a long time, and colonize new spaces in wheat rhizosphere following the extension of wheat roots. Compared with uninoculated wheat plants, those inoculated with P34-L showed significantly increased phosphorus accumulation in leaves, seedling fresh and dry weight, root fresh and dry weight, total root length, and number of root tips, forks, crossings, which showed a great value of application of the strain on wheat production by promoting the root growth and dry matter accumulation. Strain P34-L down-regulated the transcript level of TaPT4 in wheat roots, which means a well phosphorus supplementation environment was established by P34-L. 3 ImportanceMany PGPR strains often failed to achieve the desired effects when applied in the field. One major reason for the failure is lack of a special affinity between a certain strain and the target host plant, so those strains have low competitive ability with the indigenous microorganism, and unable to survive constantly in rhizosphere. In this work, a new technique to isolate wheat-specific phosphate-solubilizing PGPR strain by WGA was established. The isolate P34 was confirmed can colonize the wheat rhizosphere, and have significantly ability in promoting phosphorus absorption and wheat growth by luminescence labeling techniques. Furthermore, the phosphate-solubilizing ability of this affinity PGPR strain was verified in gene level by quantitative reverse-transcription PCR. These results lay a firm foundation for further research on the relationships between PGPR and their host plants. Meanwhile, this work supplied a potential ideal biofertilizer producing strain for sustainable agriculture.

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“…The P1BS element acts as the binding site for the PHR1 protein, a MYB transcription factor, presumably controlling the Pi starvation response in plants [55]. The substantial conservation of this candidate motif in other Pi transporter promoters in Arabidopsis, rice, barley, wheat, eggplant, and soy bean indicates that it is functionally conserved and may play a role in boosting root expression under Pi-deficient circumstances [53,[55][56][57][58][59][60]. Secondly, a number of root-specific regulatory elements, including ROOTMOTIFTAPOX1, OSE1ROOTNODULE, and OSE2ROOTNODULE, and other tissue-specific elements like CACTFTPPCA1, CAATBOX1, GTGANTG10, CCGTCC-box, and CAT-box were also discovered.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

Naveenarani,

Swamy,

Surya Krishna

et al. 2023

Plants

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Phosphorus deficiency highly interferes with plant growth and development. Plants respond to persistent P deficiency by coordinating the expression of genes involved in the alleviation of stress. Promoters of phosphate transporter genes are a great choice for the development of genetically modified plants with enhanced phosphate uptake abilities, which improve crop yields in phosphate-deficient soils. In our previous study, the sugarcane phosphate transporter PHT1;2 gene showed a significantly high expression under salinity stress. In this study, the Erianthus arundinaceus EaPHT1;2 gene was isolated and characterized using various in silico tools. The deduced 542 amino acid residues have 10 transmembrane domains, with a molecular weight and isoelectric point of 58.9 kDa and 9.80, respectively. They displayed 71–96% similarity with Arabidopsis thaliana, Zea mays, and the Saccharum hybrid. To elucidate the function of the 5′ regulatory region, the 1.1 kb promoter was isolated and validated in tobacco transgenics under Pi stress. The EaPHT1;2 promoter activity was detected using a β-glucuronidase (GUS) assay. The EaPHT1;2 promoter showed 3- to 4.2-fold higher expression than the most widely used CaMV35S promoter. The 5′ deletion analysis with and without 5′ UTRs revealed a small-sized 374 bp fragment with the highest promoter activity among 5′ truncated fragments, which was 2.7 and 4.2 times higher than the well-used CaMV35S promoter under normal and Pi deprivation conditions, respectively. The strong and short promoter of EaPHT1;2 with 374 bp showed significant expression in low-Pi-stress conditions and it could be a valuable source for the development of stress-tolerant transgenic crops.

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Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

Cited by 7 publications

References 52 publications

Root Hair Development and Adaptation to Abiotic Stress

Root Hair Development and Adaptation to Abiotic Stress

Phosphate-Solubilizing Pseudomonas sp. Strain P34-L Promotes Wheat Growth by Colonizing the Wheat Rhizosphere and Improving the Wheat Root System and Soil Phosphorus Nutritional Status

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

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