Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

Baloun, Jiří; Nevrtalova, Eva; Kováčová, Viera; Hudzieczek, Vojtěch; Čegan, Radim; Vyskot, Boris; Hobza, Roman

doi:10.1016/j.jplph.2014.04.014

Cited by 16 publications

(8 citation statements)

References 51 publications

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“…In particular, AtATX1 interacts in vivo with the metal-binding domain of RAN1, whereas its homolog AtCCH, which has an extended plant-specific carboxy-terminal domain, does not ( Puig et al, 2007 ). Recently, RAN1 has been shown to be induced by Cu supplementation in Cu-tolerant and Cu-sensitive ecotypes of S. vulgaris , suggesting a putative role of this protein in the global Cu homeostasis rather than in Cu tolerance mechanisms ( Baloun et al, 2014 ).…”

Section: Copper Trafficking In Plantsmentioning

confidence: 99%

Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics

et al. 2016

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In plants, copper (Cu) acts as essential cofactor of numerous proteins. While the definitive number of these so-called cuproproteins is unknown, they perform central functions in plant cells. As micronutrient, a minimal amount of Cu is needed to ensure cellular functions. However, Cu excess may exert in contrast detrimental effects on plant primary production and even survival. Therefore it is essential for a plant to have a strictly controlled Cu homeostasis, an equilibrium that is both tissue and developmentally influenced. In the current review an overview is presented on the different stages of Cu transport from the soil into the plant and throughout the different plant tissues. Special emphasis is on the Cu-dependent responses mediated by the SPL7 transcription factor, and the crosstalk between this transcriptional regulation and microRNA-mediated suppression of translation of seemingly non-essential cuproproteins. Since Cu is an essential player in electron transport, we also review the recent insights into the molecular mechanisms controlling chloroplastic and mitochondrial Cu transport and homeostasis. We finally highlight the involvement of numerous Cu-proteins and Cu-dependent activities in the properties of one of the major Cu-accumulation sites in plants: the cell wall.

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Section: Copper Trafficking In Plantsmentioning

confidence: 99%

Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics

et al. 2016

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“…The exceptional adaptability of S. vulgaris [32,33] has led to the occurrence of distinct ecotypes adapted to extremely unfavorable habitat conditions. Apart from ecotypes tolerant to lead [34][35][36], there are others that are known to tolerate high concentrations of zinc, cadmium, arsenic, and copper [30,35,[37][38][39][40][41]. Tolerance to chromium, nickel, and cobalt [42][43][44] has also been documented in other closely related Silene species, such as S. cobaltica [45] or S. paradoxa [46].…”

Section: Introductionmentioning

confidence: 99%

Identification of Salt and Drought Biochemical Stress Markers in Several Silene vulgaris Populations

et al. 2019

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This study attempted to determine short-term responses to drought and salt stress in different Silene vulgaris genotypes and to identify potential abiotic stress biochemical indicators in this species. Four populations from contrasting habitats were subjected to drought and three levels of salinity under controlled greenhouse conditions. The determination of several growth parameters after the stress treatments allowed for ranking the tolerance to stress of the four analyzed populations on the basis of their relative degree of stress-induced growth inhibition. This was then correlated with changes in the leaf levels of monovalent ions (Na+, Cl−, and K+), photosynthetic pigments (chlorophylls a and b, carotenoids), osmolytes (total soluble sugars, proline), and non-enzymatic antioxidants (total phenolic compounds and flavonoids). Despite the observed differences, all four populations appeared to be relatively tolerant to both stress conditions, which in general did not cause a significant degradation of photosynthetic pigments and did not generate oxidative stress in the plants. Drought and salinity tolerance in S. vulgaris was mostly dependent on the use of Na+ and K+ for osmotic adjustment under stress, a mechanism that appeared to be constitutive, and not stress-induced, since relatively high concentrations of these cations (without reaching toxic levels) were also present in the leaves of control plants. The inhibition of additional transportation of toxic ions to the leaves, in response to increasing external salinity, seemed to be a relevant mechanism of tolerance, specifically to salt stress, whereas accumulation of soluble sugars under drought conditions may have contributed to tolerance to drought.

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“…When compared with the copper‐sensitive ecotype, the constitutive expression of SvHMA7 is substantially higher in the copper‐tolerant plants. Furthermore, transcript abundance of SvHMA7 was found to be significantly higher in both ecotypes following the exposure of plants to copper excess . These data suggest that the Cu tolerance strategy of Lubietova plants involves high constitutive expression of SvHMA7 and upregulation of SvHMA7 protein in response to high levels of copper in the soil.…”

Section: Plants Copper Atpasesmentioning

confidence: 68%

Copper‐transporting ATPases: The evolutionarily conserved machineries for balancing copper in living systems

Migocka

2015

IUBMB Life

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Copper ATPases (Cu-ATPases) are ubiquitous transmembrane proteins using energy from ATP to transport copper across different biological membranes of prokaryotic and eukaryotic cells. As they belong to the P-ATPase family, Cu-ATPases contain a characteristic catalytic domain with an evolutionarily conserved aspartate residue phosphorylated by ATP to form a phosphoenzyme intermediate, as well as transmembrane helices containing a cation-binding cysteine-proline-cysteine/histidine/serine (CPx) motif for catalytic activation and cation translocation. In addition, most Cu-ATPases possess the N-terminal Cu-binding CxxC motif required for regulation of enzyme activity. In cells, the Cu-ATPases receive copper from soluble chaperones and maintain intracellular copper homeostasis by efflux of copper from the cell or transport of the metal into the intracellular compartments. In addition, copper pumps play an essential role in cuproprotein biosynthesis by the uptake of copper into the cell or delivery of the metal into the chloroplasts and thylakoid lumen or into the lumen of the secretory pathway, where the metal ion is incorporated into copper-dependent enzymes. In the recent years, significant progress has been made toward understanding the function and regulation of Cu-transporting ATPases in archaea, bacteria, yeast, humans, and plants, providing new insights into the specific physiological roles of these essential proteins in various organisms and revealing some conservative regulatory mechanisms of Cu-ATPase activity. In this review, the structural, biochemical, and functional properties of Cu-ATPases from phylogenetically different organisms are summarized and discussed, with particular attention given to the recent insights into the molecular biology of copper pumps in plants. V C 2015 IUBMB Life, 67(10):737-745, 2015

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Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

Cited by 16 publications

References 51 publications

Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics

Copper Trafficking in Plants and Its Implication on Cell Wall Dynamics

Identification of Salt and Drought Biochemical Stress Markers in Several Silene vulgaris Populations

Copper‐transporting ATPases: The evolutionarily conserved machineries for balancing copper in living systems

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