Characterization of the human brain putative A<sub>2B</sub> adenosine receptor expressed in Chinese hamster ovary (CHO.A<sub>2B4</sub>) cells

Alexander, Stephen; Cooper, Joan; Shine, John; Hill, Stephen J.

doi:10.1111/j.1476-5381.1996.tb16035.x

Cited by 49 publications

(57 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the cord and chorionic vessels the receptors are mainly on smooth muscle, while in the cotyledon vessels P2Y 1 and P2Y 2 receptors were equally distributed between endothelial and smooth muscle cells. mRNA for P2Y 4 , P2Y 6 and P2Y 11 receptors was also found. P2X1, P2X4 and P2X7 receptors were also present on the smooth muscle of human chorionic blood vessels [408].…”

Section: Placentamentioning

confidence: 93%

“…It was also shown that functional expression of human CFTR in the plasma membrane of CHO cells infected with adenovirus regulated the increase in intracellular Ca 2+ produced by ATP released from the cells. A 2B receptors have also been identified on CHO cells [6].…”

Section: Female Reproductive Organsmentioning

confidence: 97%

“…A later study by this group showed that treatment of cultured Sertoli cells with pertussis toxin reversed the adenosine-mediated inhibition of cyclic AMP (cAMP) accumulation and potentiated the cAMP response to follicle stimulating hormone (FSH) [185,285]. A low affinity binding site for N 6 -substituted adenosine derivatives in a rat testis membrane preparation showed the typical pharmacological profile of the cloned rat A 3 receptor [265]. The TM4 cell line is derived from a primary culture of Sertoli cells isolated from mouse testis.…”

Section: Testismentioning

confidence: 99%

“…It was claimed that ATP released from nerves produces relaxation of cavernosum smooth muscle via P2Y 4 receptors on smooth muscle, while ADP acting via P2Y 1 receptors on endothelial cells produces relaxation via NO [72]. Modulation of cavernosum smooth muscle relaxation occurs by activation of P2Y 6 receptors via nonneuronal and non-NO-dependent mechanisms [224]. It was suggested that ADP-sensitive P2Y 12 and/or P2Y 13 receptors might mediate relaxation of corpus cavernosum through the release of prostanoids [112].…”

Section: Penis and Penile Erectionmentioning

confidence: 99%

See 3 more Smart Citations

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

View full text Add to dashboard Cite

There are multiple roles for purinergic signalling in both male and female reproductive organs. ATP, released as a cotransmitter with noradrenaline from sympathetic nerves, contracts smooth muscle via P2X1 receptors in vas deferens, seminal vesicles, prostate and uterus, as well as in blood vessels. Male infertility occurs in P2X1 receptor knockout mice. Both short-and long-term trophic purinergic signalling occurs in reproductive organs. Purinergic signalling is involved in hormone secretion, penile erection, sperm motility and capacitation, and mucous production. Changes in purinoceptor expression occur in pathophysiological conditions, including pre-eclampsia, cancer and pain.

show abstract

Section: Placentamentioning

confidence: 93%

Section: Female Reproductive Organsmentioning

confidence: 97%

Section: Testismentioning

confidence: 99%

Section: Penis and Penile Erectionmentioning

confidence: 99%

See 2 more Smart Citations

Purinergic signalling in the reproductive system in health and disease

Burnstock

2013

Purinergic Signalling

View full text Add to dashboard Cite

show abstract

“…The inclusion of 300 M IBMX should block P1 receptor activation as this compound is a general low potency P1 antagonist (Fredholm et al, 1994). However, to eliminate this possibility we stimulated cells with 2-MeSADP in the additional presence of the nonselective P1 antagonist CGS 15943 (Alexander et al, 1996). This treatment had no significant effect on the ability of 2-MeSADP to inhibit glucagon-stimulated cyclic AMP levels.…”

Section: Activation Of Glycogen Phosphorylase By 2-mesadp In Freshly mentioning

confidence: 99%

Regulation of Rat Hepatocyte Function by P2Y Receptors: Focus on Control of Glycogen Phosphorylase and Cyclic AMP by 2-Methylthioadenosine 5′-Diphosphate

Dixon

Hall²,

Webb³

et al. 2004

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Hepatocyte function is regulated by several P2Y receptor subtypes. Here we report that 2-methylthioadenosine 5Ј-diphosphate (2-MeSADP), an agonist at P2Y 1 , P2Y 12 , and P2Y 13 receptors, potently (threshold 30 nM) stimulates glycogen phosphorylase in freshly isolated rat hepatocytes. Antagonism by N 6 -methyl 2Ј-deoxyadenosine 3Ј,5Ј-bisphosphate (MRS 2179) confirms that this response is mediated by P2Y 1 receptors. In addition, in these cells, both 2-MeSADP and UTP inhibited glucagon-stimulated cyclic AMP accumulation. This inhibitory effect of 2-MeSADP was not reversed by the P2Y 1 antagonists, adenosine-3Ј-phosphate-5Ј-phosphate (A3P5P) or MRS 2179, both in the range 1 to 300 M, indicating that it was not mediated by P2Y 1 receptors. This contrasts with the increase in cytosolic free Ca 2ϩ concentration ([Ca 2ϩ ] c ) induced by 2-MeSADP, which has shown to be inhibited by A3P5P. Pertussis toxin abolished the inhibitory effect of both UTP and 2-MeSADP. After culture of cells for 48 h, the ability of 2-Me-SADP to inhibit cyclic AMP accumulation was greatly diminished. Reverse transcriptase-polymerase chain reaction analysis revealed that during this culture period, there was a decline in the ability to detect transcripts for P2Y 12 and P2Y 13 receptors, both of which are activated by 2-MeSADP and negatively coupled to adenylyl cyclase. However, in freshly isolated cells, the P2Y 12 and P2Y 13 receptor antagonist, 2-propylthio-␤,␥-dichloromethylene-D-ATP (AR-C67085) (10 nM to 300 M) did not alter the ability of 2-MeSADP to inhibit glucagon-stimulated cyclic AMP accumulation. We conclude that 2-MeSADP regulates rat hepatocyte glycogen phosphorylase by acting on P2Y 1 receptors coupled to raised [Ca 2ϩ ] c , and by inhibiting cyclic AMP levels by an unknown G i -coupled receptor subtype, distinct from P2Y 1 , P2Y 12 , or P2Y 13 receptors.Glycogen phosphorylase, the rate-controlling enzyme in hepatic glycogenolysis, is activated by increases in both cyclic AMP and cytosolic free Ca 2ϩ concentration ([Ca 2ϩ ] c ), resulting in a net output of glucose to supply extrahepatic tissues, crucially the brain, when blood glucose levels drop. Extracellular nucleotides play a well established role in the regulation of this key function in rat hepatocytes through the activation of P2Y receptors (Okajima et al., 1987;Keppens et al., 1992Keppens et al., , 1993Keppens, 1993), a family of G-protein-coupled receptors responding to the native nucleotides ADP, ATP, UDP, UTP, and UDP-glucose (Boarder and Hourani, 1998;Abbracchio et al., 2003). Curiously, stimulation of P2Y receptors on rat hepatocytes leads to both increases in [Ca 2ϩ ] c and inhibition of adenylyl cyclase; these responses will stimulate and limit, respectively, activation of glycogen phosphorylase (Okajima et al., 1987;Dixon et al., 1990Dixon et al., , 1995Dixon et al., , 2000Keppens et al., 1992;1993;Keppens, 1993;Edgecombe et al., 1999). The mechanism underlying the regulation of glycogen phosphorylase by cyclic AMP is well established, with ...

show abstract

Potentiation of ATP calcium responses by A2B receptor stimulation and other signals coupled to Gs proteins in type-1 cerebellar astrocytes

Jim�nez

Castro

Mirabet

et al. 1999

Glia

View full text Add to dashboard Cite

We have studied the interaction between P1 and P2 purinoceptors in purified type‐1 astrocyte cultures from postnatal days 7–8 rat cerebella using single cell microfluorimetry with fura‐2. The stimulation of astrocytes with ATP elicits rapid [Ca2+]i transients showing an EC50 value of 7.9 ± 0.3 μM. Costimulation of type‐1 astrocytes with adenosine and ineffective ATP concentrations (0.1 or 1 μM) evoked [Ca2+]i transients that correspond to 60% of the maximal ATP response. NECA (5′‐N‐ethylcarboxamidoadenosine) was the only agonist that mimicked the adenosine effect and showed an EC50 value of 0.17 ± 0.01 μM. This value was identical to that obtained for the cAMP production stimulation, indicating that A2B receptors coupled to adenylate cyclase activation were involved. The presence of A2B adenosine receptors was also confirmed by immocytochemistry experiments. When astrocytes were costimulated with isoproterenol and ineffective ATP concentrations similar [Ca2+]i transients were observed. The treatment of astrocytes with cholera toxin potentiated ATP calcium signals, lowering the EC50 value for ATP to 1.5 ± 0.2 μM. However, the pretreatment of cells with forskolin or a permeable cAMP analogue had no effect on ATP calcium responses. These results indicated that the potentiation mechanism was elicited before the adenylate cyclase activation. We could conclude that in type‐1 astrocytes, the activation of A2B adenosine receptors or other signals positively coupled to adenylate cyclase stimulation strongly potentiate metabotropic calcium responses to ATP. The potentiation was parallel but independent on cAMP accumulation suggesting the involvement of βγ subunits released after Gs stimulation. GLIA26:119–128, 1999. © 1999 Wiley‐Liss, Inc.

show abstract

Characterization of the human brain putative A_2B adenosine receptor expressed in Chinese hamster ovary (CHO.A_2B4) cells

Cited by 49 publications

References 14 publications

Purinergic signalling in the reproductive system in health and disease

Purinergic signalling in the reproductive system in health and disease

Regulation of Rat Hepatocyte Function by P2Y Receptors: Focus on Control of Glycogen Phosphorylase and Cyclic AMP by 2-Methylthioadenosine 5′-Diphosphate

Potentiation of ATP calcium responses by A2B receptor stimulation and other signals coupled to Gs proteins in type-1 cerebellar astrocytes

Contact Info

Product

Resources

About

Characterization of the human brain putative A2B adenosine receptor expressed in Chinese hamster ovary (CHO.A2B4) cells

Cited by 49 publications

References 14 publications

Purinergic signalling in the reproductive system in health and disease

Purinergic signalling in the reproductive system in health and disease

Regulation of Rat Hepatocyte Function by P2Y Receptors: Focus on Control of Glycogen Phosphorylase and Cyclic AMP by 2-Methylthioadenosine 5′-Diphosphate

Potentiation of ATP calcium responses by A2B receptor stimulation and other signals coupled to Gs proteins in type-1 cerebellar astrocytes

Contact Info

Product

Resources

About

Characterization of the human brain putative A_2B adenosine receptor expressed in Chinese hamster ovary (CHO.A_2B4) cells