1998
DOI: 10.1007/s007050050370
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Characterization of the human cytomegalovirus UL97 gene product as a virion-associated protein kinase

Abstract: The cellular localization and virion association of the human cytomegalovirus (HCMV) UL97 protein were studied. UL97 protein demonstrated early nuclear localization followed by late perinuclear accumulation. It was found to be a structural virion constituent detected in all three enveloped forms of extracellular viral particles and shown to be phosphorylated by the virion-associated protein kinase. UL97 protein immunoprecipitated from virions and from infected cells demonstrated protein kinase activity manifes… Show more

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Cited by 28 publications
(24 citation statements)
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“…We observed kinase-dependent changes beginning at 1 hpi (Fig. 4D), suggesting a role for tegument-delivered pUL97 (5,6). Supporting this possibility, transient expression of pUL97 but not inactive kinase was sufficient to enhance MIE promoter activity following transfection of UL97-deficient HCMV genomes into cells (Fig.…”
Section: Discussionmentioning
confidence: 63%
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“…We observed kinase-dependent changes beginning at 1 hpi (Fig. 4D), suggesting a role for tegument-delivered pUL97 (5,6). Supporting this possibility, transient expression of pUL97 but not inactive kinase was sufficient to enhance MIE promoter activity following transfection of UL97-deficient HCMV genomes into cells (Fig.…”
Section: Discussionmentioning
confidence: 63%
“…This modification is necessary for activating ganciclovir's antiviral activity (3,4). pUL97 is a tegument protein delivered to infected cells, and newly expressed pUL97 protein begins increasing around 5 hours postinfection (hpi) (5,6). The kinase exists in multiple isoforms, which have distinct expression patterns within the cell (7,8).…”
mentioning
confidence: 99%
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“…The intracellular localization of pUL97 has been analysed in detail by previous studies (Michel et al, 1996(Michel et al, , 1998Wolf et al, 1998;Marschall et al, 2003Marschall et al, , 2005Prichard et al, 2005;Romaker et al, 2006;Hamirally et al, 2009;Milbradt et al, 2009Milbradt et al, , 2010. A predominantly nuclear and perinuclear localization of pUL97 occurs in HCMV-infected cells (Michel et al, 1996;Marschall et al, 2003), with concentrations of the protein kinase occurring at the nuclear rim during the late phase of infection (Milbradt et al, 2010;Marschall et al, 2005;M.…”
Section: Results and Discussion Pul97 Occurs In Two Isoformsmentioning
confidence: 98%
“…In alphaherpesviruses, pUL97 homologs are dispensable for viral replication in cell culture (4,6,10,23), whereas their function is thought to be relevant for betaherpesviruses (9,16,22,31). Although the role of pUL97 in the biology of HCMV is still unknown, the protein has attracted much interest since it has been shown to activate GCV as well as other antiviral nucleoside analogs by converting them to their monophosphates and because specific mutations in the UL97 ORF confer drug resistance on the virus.…”
Section: Discussionmentioning
confidence: 99%