Characterization of the
            <i>Aspergillus nidulans nmrA</i>
            Gene Involved in Nitrogen Metabolite Repression

Andrianopoulos, Alex; Kourambas, Sophie; Sharp, Julie A.; Davis, Meryl A.; Hynes, Michael J.

doi:10.1128/jb.180.7.1973-1977.1998

Cited by 141 publications

(81 citation statements)

References 43 publications

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“…Currently, transcriptome analysis is in progress of F. fujikuroi areA and areB mutants grown under various nitrogen conditions to determine common and different target genes on a genome-wide scale. Alternatively, it is intriguing to speculate that the role of AreB has diverged between A. nidulans and F. fujikuroi, as it is the case for NmrA and the bZIP transcription factor MeaB (Andrianopoulos et al, 1998;Mihlan et al, 2003;Wong et al, 2007;Schönig et al, 2008;Wagner et al, 2010). Indeed, cross-species complementation revealed that the role of AreB is only partially conserved in the two fungal species.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been shown that this process depends on the RNA-binding protein RrmA (Król et al, 2013). At the protein level, the function of AreA/Nit2 is negatively regulated by binding to the nitrogen metabolite repressor NmrA under nitrogen repressing conditions, and deletion of nmrA led to partial de-repression of nitrogen-regulated genes (Jarai and Marzluf, 1990;Andrianopoulos et al, 1998;Lamb et al, 2003;Kotaka et al, 2008). In F. fujikuroi, it has also been shown that AreA interacts with FfNmr, a homologue of A. nidulans NmrA.…”

Section: Introductionmentioning

confidence: 99%

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The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Michielse¹,

Pfannmüller²,

Macios

et al. 2013

Molecular Microbiology

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SummaryNitrogen metabolite repression (NMR) in filamentous fungi is controlled by the GATA transcription factors AreA and AreB. While AreA mainly acts as a positive regulator of NMR-sensitive genes, the role of AreB is not well understood. We report the characterization of AreB and its interplay with AreA in the gibberellinproducing fungus Fusarium fujikuroi. The areB locus produces three different transcripts that each code for functional proteins fully complementing the areB deletion mutant that influence growth and secondary metabolism. However, under nitrogen repression, the AreB isoforms differ in subcellular localization indicating distinct functions under these conditions. In addition, AreA and two isoforms of AreB colocalize in the nucleus under low nitrogen, but their nuclear localization disappears under conditions of high nitrogen. Using a bimolecular fluorescence complementation (BiFC) approach we showed for the first time that one of the AreB isoforms interacts with AreA when starved of nitrogen. Cross-species complementation revealed that some AreB functions are retained between F. fujikuroi and Aspergillus nidulans while others have diverged. By comparison to other fungi where AreB was postulated to function as a negative counterpart of AreA, AreB can act as both repressor and activator of transcription in F. fujikuroi.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Michielse¹,

Pfannmüller²,

Macios

et al. 2013

Molecular Microbiology

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“…Unigene 95 encoded an NmrAlike protein that was specifically expressed during biotrophic growth in planta (Figure 4). NmrA is a repressor of the AreA global regulator of nitrogen metabolism (Andrianopoulos et al, 1988), and an AreA orthologue, CLNR1, has been shown to be essential for pathogenicity and the biotrophy to necrotrophy switch in C. lindemuthianum (Pellier et al, 2003). A homologue of the N. crassa CPC2 cross-pathway control gene (Mü ller et al, 1995) was also present in the library (unigene 114).…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric purification ofColletotrichum higginsianumbiotrophic hyphae from Arabidopsis leaves for stage‐specific transcriptome analysis

et al. 2009

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SUMMARYGeneration of stage-specific cDNA libraries is a powerful approach to identify pathogen genes that are differentially expressed during plant infection. Biotrophic pathogens develop specialized infection structures inside living plant cells, but sampling the transcriptome of these structures is problematic due to the low ratio of fungal to plant RNA, and the lack of efficient methods to isolate them from infected plants. Here we established a method, based on fluorescence-activated cell sorting (FACS), to purify the intracellular biotrophic hyphae of Colletotrichum higginsianum from homogenates of infected Arabidopsis leaves. Specific selection of viable hyphae using a fluorescent vital marker provided intact RNA for cDNA library construction. Pilot-scale sequencing showed that the library was enriched with plant-induced and pathogenicity-related fungal genes, including some encoding small, soluble secreted proteins that represent candidate fungal effectors. The high purity of the hyphae (94%) prevented contamination of the library by sequences derived from host cells or other fungal cell types. RT-PCR confirmed that genes identified in the FACS-purified hyphae were also expressed in planta. The method has wide applicability for isolating the infection structures of other plant pathogens, and will facilitate cell-specific transcriptome analysis via deep sequencing and microarray hybridization, as well as proteomic analyses.

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“…In the absence of ammonium, the process of nitrogen assimilation from less preferred sources, such as nitrate, urea, peptides, and amino acids, are regulated by nitrogen metabolite repression (Tudzynski, 2014). In ascomycota, nitrogen metabolite repression is controlled by a GATA transcription factor (Caddick, 1992), which is under the regulation of a co-repressor NmrA (Fu et al, 1988;Andrianopoulos et al, 1998). Additionally, bZIP transcription factors have been also shown to be involved.…”

Section: Effect Of N or P Starvation On Cellular Proteome In Serendipmentioning

confidence: 99%

Scavenging organic nitrogen and remodelling lipid metabolism are key survival strategies adopted by the endophytic fungi, Serendipita vermifera and Serendipita bescii to alleviate nitrogen and phosphorous starvation in vitro

Ray

Abraham

Guo

et al. 2019

Environ Microbiol Rep

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Summary Serendipitaceae represents a diverse fungal group in the Basidiomycota that includes endophytes and lineages that repeatedly evolved ericoid, orchid and ectomycorrhizal lifestyle. Plants rely upon both nitrogen and phosphorous, for essential growth processes, and are often provided by mycorrhizal fungi. In this study, we investigated the cellular proteome of Serendipita vermifera MAFF305830 and closely related Serendipita vermifera subsp. bescii NFPB0129 grown in vitro under (N) ammonium and (P) phosphate starvation conditions. Mycelial growth pattern was documented under these conditions to correlate growth‐specific responses to nutrient starvation. We found that N‐starvation accelerated hyphal radial growth, whereas P‐starvation accelerated hyphal branching. Additionally, P‐starvation triggers an integrated starvation response leading to remodelling of lipid metabolism. Higher abundance of an ammonium transporter known to serve as both an ammonium sensor and stimulator of hyphal growth was detected under N‐starvation. Additionally, N‐starvation led to strong up‐regulation of nitrate, amino acid, peptide, and urea transporters, along with several proteins predicted to have peptidase activity. Taken together, our finding suggests S. bescii and S. vermifera have the metabolic capacity for nitrogen assimilation from organic forms of N compounds. We hypothesize that the nitrogen metabolite repression is a key regulator of such organic N assimilation.

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Characterization of the Aspergillus nidulans nmrA Gene Involved in Nitrogen Metabolite Repression

Cited by 141 publications

References 43 publications

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

The interplay between the GATA transcription factors AreA, the global nitrogen regulator and AreB in Fusarium fujikuroi

Flow cytometric purification ofColletotrichum higginsianumbiotrophic hyphae from Arabidopsis leaves for stage‐specific transcriptome analysis

Scavenging organic nitrogen and remodelling lipid metabolism are key survival strategies adopted by the endophytic fungi, Serendipita vermifera and Serendipita bescii to alleviate nitrogen and phosphorous starvation in vitro

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