Characterization of the Xanthomonas sp. causing wilt of enset and banana and its proposed reclassification as a strain of X. vasicola

Abstract

Comparative analyses were undertaken to characterize Xanthomonas campestris pv. musacearum, the causal agent of a wilt of enset and banana, and to assess its relatedness to other xanthomonads by fatty acid methyl esters, genomic fingerprinting using rep-PCR and partial nucleotide sequencing of the gyrase B gene. The results from all three analyses indicated that strains of X. campestris pv. musacearum are homogeneous and very similar to X. vasicola strains isolated from sugarcane and maize from Africa. Pathogenicity studies indicated that strains of X. vasicola pv. holcicola and X. vasicola from sugarcane induced no symptoms on banana, whereas X. campestris pv . musacearum produced severe disease. These data will support a future proposed reclassification of X. campestris pv. musacearum as X. vasicola pv . musacearum when more data are available.

Evaluation of banana genotype resistant to Xanthomonas wilts disease (Xanthomonas campestris pv. musacearum) in south east of Ethiopia

“…However, in recent years, the epidemics of Xanthomonas wilt with significant damage have been reported on banana in Uganda in 2001 (Tushemereirwe et al, 2004). Further outbreak and establishment were also confirmed on banana in eastern Congo, in the Lake Victoria region of Tanzania, Rwanda and Kenya (Aritua et al, 2008;Biruma et al, 2007 andNdungo et al, 2006). Banana production losses caused by this pathogen threaten the food security of about 100 million people and the income of millions of farmers in the Great Lakes region of Central and Eastern Africa, who depend on banana fruit for food and export trade (Tripathi et al, 2009) and also threatens food security of over 15 million of Ethiopians" who utilize Enset as a staple or co-staple food (Brandt et al, 1997).…”

Safety Assessment of Transgenic Organisms, Volume 4

“…Banana Xanthomonas wilt (or banana bacterial wilt) is a devastating disease emerging in East Africa to which most cultivars appear vulnerable; it is caused by Xanthomonas vasicola pv. musacearum (Biruma et al, 2007;Aritua et al, 2008). Symptoms are somewhat cultivar-specific and determined by route and stage of infection.…”

“…musacearum (Biruma et al, 2007;Aritua et al, 2008). Symptoms are somewhat cultivar-specific and determined by route and stage of infection.…”

Banana Xanthomonas Wilt: Occurrence and Means of Transmission in Kagera, Mwanza and Mara Regions of Tanzania

“…Pure bacterial isolates were identified using physiological and biochemical characteristics, including colony morphology on YPGA, Gram reaction, Kovac"s oxidase reaction, nitrate reduction and pathogenecity test) [2] …”