Laser-induced modification at 355 nm of deuterated potassium dihydrogen phosphate (DKDP) crystals following exposure to nanosecond (ns) and sub-ns laser irradiation is investigated in order to probe the absorption mechanism in damage initiation. Laser damage resistance is greatly improved by sub-ns laser conditioning, whereas only a little improvement occurred after ns laser conditioning at the same laser fluence. Moreover, scattering and transmittance variations after the two types of laser conditioning indicate similar reduction of linear absorption. However, by contrast, large differences on nonlinear absorption modification are discovered using Z-scan measurement. This characteristic absorption modification by laser irradiation provides evidence that a nonlinear absorption mechanism plays a key role in damage initiation at 355 nm.