2022
DOI: 10.3389/fendo.2022.910639
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Characterization of the Interrenal Gland and Sexual Traits Development in cyp17a2-Deficient Zebrafish

Abstract: Unlike the Cytochrome P450, family 17, subfamily A, member 1 (Cyp17a1), which possesses both 17α-hydroxylase and 17,20-lyase activities involved in the steroidogenic pathway that produces androgens and estrogens, Cytochrome P450, family 17, subfamily A, polypeptide 2 (Cyp17a2) possesses only 17α-hydroxylase activity and is known essential for the synthesis of cortisol. Besides with expressed in testes and ovaries, where the cyp17a1 is mainly expressed, cyp17a2 is also expressed in the interrenal gland in fish.… Show more

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Cited by 5 publications
(3 citation statements)
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“…Through functional study, our present study further proved that DHP and cortisol played an essential role in spermiogenesis and fertility in male fish. In zebrafish, mutation of cyp17a2 gene resulted in the enlargement of interrenal gland, up-regulated expression of cyp11a1 , cyp21a1 , and hsd3b1 in the interrenal gland, and significant decrease of cortisol biosynthesis ( 50 ). However, zebrafish cyp17a2 gene mutation had no effects on fertility and secondary sex characteristics in males.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Through functional study, our present study further proved that DHP and cortisol played an essential role in spermiogenesis and fertility in male fish. In zebrafish, mutation of cyp17a2 gene resulted in the enlargement of interrenal gland, up-regulated expression of cyp11a1 , cyp21a1 , and hsd3b1 in the interrenal gland, and significant decrease of cortisol biosynthesis ( 50 ). However, zebrafish cyp17a2 gene mutation had no effects on fertility and secondary sex characteristics in males.…”
Section: Discussionmentioning
confidence: 99%
“…However, zebrafish cyp17a2 gene mutation had no effects on fertility and secondary sex characteristics in males. Furthermore, abnormal increase of progesterone, testosterone, DHP, and 11-KT in the cyp17a2 -deficient males than those in wild-type male fish was observed ( 50 ). We speculated that discrepancy of DHP production and male fertility between cyp17a2 deficiency zebrafish and tilapia might be due to the species difference.…”
Section: Discussionmentioning
confidence: 99%
“…500 ng of the RNA template was used for reverse transcription and cDNA synthesis using the EasyScript One-Step gDNA Removal and cDNA Synthesis SuperMix Kit (AE311-03, Transgen, China). The qPCR was conducted using PerfectStartTM Green qPCR SuperMix (AQ601-02, Transgen, China) according to a previous study (Shi et al, 2022). qPCR was conducted using a Bio-Rad Real-Time System (Bio-Rad Systems, USA).…”
Section: Rna Extraction and Quantitative Real-time Polymerase Chain R...mentioning
confidence: 99%