Characterization of the Jasmonate Biosynthetic Gene Allene Oxide Cyclase in Artemisia annua L., Source of the Antimalarial Drug Artemisinin

Lu, Xu; Lin, Xiuyan; Shen, Qian; Zhang, Fangyuan; Wang, Yueyue; Chen, Yunfei; Wang, Tao; Wu, Shaoyan; Tang, Kexuan

doi:10.1007/s11105-010-0252-7

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…In addition, the monocotyledonous AOCs from rice, L. mollis, wheat, barley, maize, and sorghum were clustered together in Group II. Four AtAOC proteins together with GhAOC1 were clustered in Group III and diverged earlier than other species (Figure 3), which is consistent with the previous reports [9,33].…”

Section: Phylogenetic and Structural Analyses Of Aoc Genes From Diffesupporting

confidence: 91%

“…To characterize ClAOC1 and ClAOC2, a multiple sequence alignment was performed for their full-length amino acid sequences, as well as other published AOC proteins, such as SlAOC from Solanum lycopersicum [6], HvAOC from Hordeum vulgare [13], HnAOC from Hyoscyamus niger [31], JcAOC from Jatropha curcas [32], AaAOC from Artemisia annua [33], OsAOC from Oryza sativa [12], LmAOC from Leymus mollis [34], TaAOC1 from Triticum aestivum [17], GhAOC1 from Gossypium hirsutum [8], GmAOC3 from Glycine max [18], CsAOC from Camellia sinensis [35], and Ljaoc1 from Lotus japonicus [10]. The results showed that the identity between ClAOC1 and ClAOC2 was 62.13%, and the amino acid sequences of ClAOC1 and ClAOC2 shared 58.05% to 69.08% identity with other AOC proteins ( Figure 2).…”

Section: Characterization Of Claoc1 and Claoc2mentioning

confidence: 99%

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Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Guang

Yang

et al. 2019

Agriculture

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Allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step in the jasmonic acid (JA) biosynthetic pathway and mediates plant defense response to a wide range of biotic and abiotic stresses. In this study, two AOC genes were identified from watermelon. Sequence analysis revealed that each of ClAOC1 and ClAOC2 contained an allene oxide cyclase domain and comprised eight highly conserved β-strands, which are the typical characteristics of AOC proteins. Phylogenetic analysis showed that ClAOC1 and ClAOC2 were clustered together with AOCs from dicotyledon, with the closest relationships with JcAOC from Jatropha curcas and Ljaoc1 from Lotus japonicus. Different intron numbers were observed in ClAOC1 and ClAOC2, which may result in their functional divergence. qRT-PCR analysis revealed that ClAOC1 and ClAOC2 have specific and complex expression patterns in multiple organs and under hormone treatments. Both ClAOC1 and ClAOC2 displayed the highest transcriptional levels in stem apex and fruit and exhibited relatively lower expression in stem. JA, salicylic acid (SA), and ethylene (ET) could enhance the expression of ClAOC1 and ClAOC2, particularly that of ClAOC2. Red light could induce the expression of ClAOC2 in root-knot nematode infected leaf and root of watermelon, indicating that ClAOC2 might play a primary role in red light-induced resistance against root-knot nematodes through JA signal pathway. These findings provide important information for further research on AOC genes in watermelon.Agriculture 2019, 9, 225 2 of 12 AOC genes were found to have specific and complicated tissue expression patterns in different plants. In barley, HvAOC mRNA accumulation is abundant in root tip, scutellar node, and leaf base [13]. Soybean GmAOC1 and GmAOC2 also exhibited abundant mRNA transcripts in roots, while GmAOC3 and GmAOC4 showed relatively higher expression in leaves and stems, respectively [7]. Camptotheca acuminata CaAOC is constitutively expressed in various organs, with the highest expression level in leaves [14]. In Arabidopsis, AtAOC1, AtAOC2, and AtAOC3 promoters exhibit high activities in the leaves, while only AtAOC3 and AtAOC4 show promoter activity in roots [15]. These results suggest that AOC genes are involved in the regulation of multiple plant developmental processes.The expression of jasmonate biosynthetic pathway genes, including AOCs, is regulated by JA, and overexpression or suppression of these genes can greatly affect the JA levels in plants. For example, a severe deficiency of jasmonate was found in two AOC mutants (cpm2 and hebiba) of rice [12], and partial suppression of MtAOC1 in hairy roots of Medicago truncatula also resulted in lower JA levels in mycorrhizal roots [16]. In addition, elevated JA levels were found in transgenic plants overexpressing AOC genes from different plants, such as AaAOC from Artemisia annua [5], TaAOC1 from wheat [17], and GmAOC3 from soybean [18]. JA and its related compounds are involved in plant defense reactions against biotic and abiotic stresses, and th...

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Section: Phylogenetic and Structural Analyses Of Aoc Genes From Diffesupporting

confidence: 91%

Section: Characterization Of Claoc1 and Claoc2mentioning

confidence: 99%

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Guang

Yang

et al. 2019

Agriculture

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“…Therefore, tomato fruit of RNAi lines displayed deep red coloration compared to the control fruit during ripening (Sun et al, 2012). ABA treatment could also increase the production of artemisinin (Lu et al, 2011). The full-length cDNA of ABA receptor AaPYL9 was cloned and characterized from A. annua .…”

Section: Plant Metabolic Engineering Strategies For Production Of Phamentioning

confidence: 99%

Plant Metabolic Engineering Strategies for the Production of Pharmaceutical Terpenoids

Tang

2016

Front. Plant Sci.

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101

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Pharmaceutical terpenoids belong to the most diverse class of natural products. They have significant curative effects on a variety of diseases, such as cancer, cardiovascular diseases, malaria and Alzheimer’s disease. Nowadays, elicitors, including biotic and abiotic elicitors, are often used to activate the pathway of secondary metabolism and enhance the production of target terpenoids. Based on Agrobacterium-mediated genetic transformation, several plant metabolic engineering strategies hold great promise to regulate the biosynthesis of pharmaceutical terpenoids. Overexpressing terpenoids biosynthesis pathway genes in homologous and ectopic plants is an effective strategy to enhance the yield of pharmaceutical terpenoids. Another strategy is to suppress the expression of competitive metabolic pathways. In addition, global regulation which includes regulating the relative transcription factors, endogenous phytohormones and primary metabolism could also markedly increase their yield. All these strategies offer great opportunities to enhance the supply of scarce terpenoids drugs, reduce the price of expensive drugs and improve people’s standards of living.

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“…MeJA, an important plant defense response signaling molecules, is extensively involved in the activation of plant defense-related genes (Turner et al 2002;Lu et al 2011). T. chinensis cells were treated with 100 μM MeJA; then, the mRNA accumulation of TcLOX1 and TcLOX2 was detected.…”

Section: Expression Analysis Of Tclox1 and Tclox2mentioning

confidence: 99%

Molecular Cloning and Characterization of Two 9-Lipoxygenase Genes from Taxus chinensis

Zhang

et al. 2012

Plant Mol Biol Rep

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Plant lipoxygenases (LOXs) are functionally diverse class of dioxygenases involved in multiple physiological processes such as plant growth, biotic and abiotic stress responses, and secondary metabolite accumulation. In this paper, two LOX genes, TcLOX1 and TcLOX2, were cloned from Taxus chinensis cells. Multiple alignment of the deduced amino acid sequences with those of other plants demonstrated the putative LH2/PLAT domain, lipoxygenase iron-binding catalytic domain, lipoxygenase_1 and lipoxygenase_2 signature sequences. Phylogenetic analysis suggested that TcLOX1 and TcLOX2 putative proteins are most probably 9-LOXs, and shared the highest identity with the tea plant CsLOX1 and Picea sitchensis LOX genes, respectively. Semiquantitative RT-PCR analysis showed that TcLOX1 was preferentially expressed in stem and root, while TcLOX2 was preferentially expressed in root. Real-time quantitative PCR analysis showed that a strong upregulation of TcLOX1 was observed in response to methyl jasmonate and abscisic acid (ABA), while TcLOX2 was strongly upregulated by ABA. However, TcLOX1 and TcLOX2 were nearly not responding to salicylic acid. These data suggest both TcLOX1 and TcLOX2 play an important role in T. chinensis, and they are required in different physiological processes involved in different plant signals in vivo.

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Characterization of the Jasmonate Biosynthetic Gene Allene Oxide Cyclase in Artemisia annua L., Source of the Antimalarial Drug Artemisinin

Cited by 14 publications

References 27 publications

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Plant Metabolic Engineering Strategies for the Production of Pharmaceutical Terpenoids

Molecular Cloning and Characterization of Two 9-Lipoxygenase Genes from Taxus chinensis

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