Characterization of the lipid accumulation in a tropical freshwater microalgae Chlorococcum sp.

Harwati, Theresia Umi; Willke, Thomas; Vorlop, Klaus‐Dieter

doi:10.1016/j.biortech.2012.06.098

Cited by 51 publications

(28 citation statements)

References 31 publications

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“…Results clearly indicated that the variables had a significant influence on the productivity of biomass. Similar correlation was reported by Harwati et al (2012).…”

Section: Resultssupporting

confidence: 92%

Growth kinetics of Chlorococcum humicola – A potential feedstock for biomass with biofuel properties

Thomas

Jayachithra

2015

Ecotoxicology and Environmental Safety

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“…Results clearly indicated that the variables had a significant influence on the productivity of biomass. Similar correlation was reported by Harwati et al (2012).…”

Section: Resultssupporting

confidence: 92%

Growth kinetics of Chlorococcum humicola – A potential feedstock for biomass with biofuel properties

Thomas

Jayachithra

2015

Ecotoxicology and Environmental Safety

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“…As indicated in Table 2, when undergoing nitrogen depletion the lipid accumulated in strain JSC4 tended to contain more oleic acid (C18:1), while the linolenic acid (C18:3) content sharply decreased. In terms of biodiesel properties, oils containing a higher percentage of oleic acid provide better oxidation stability with a suitable cold filter plugging point in cold weather [27]. In addition, based on the European Biodiesel Standard EN14214, the content of linolenic acid (C18:3) is limited to a maximum of 12% [28].…”

Section: Resultsmentioning

confidence: 99%

“…Increases in salinity may result in a significant increase in the lipid content of several microalgal species, including Chlorococcum sp. [27], Dunaliella sp. [14], Botryococcus braunii [32], Chlorella protothecoides [15], and Nannochloropsis sp.…”

Section: Resultsmentioning

confidence: 99%

Optimizing biodiesel production in marine Chlamydomonassp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy

Nakanishi

et al. 2014

Biotechnol Biofuels

110

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BackgroundBiodiesel production from marine microalgae has received much attention as microalgae can be cultivated on non-arable land without the use of potable water, and with the additional benefits of mitigating CO2 emissions and yielding biomass. However, there is still a lack of effective operational strategies to promote lipid accumulation in marine microalgae, which are suitable for making biodiesel since they are mainly composed of saturated and monounsaturated fatty acids. Moreover, the regulatory mechanisms involved in lipid biosynthesis in microalgae under environmental stress are not well understood.ResultsIn this work, the combined effects of salinity and nitrogen depletion stresses on lipid accumulation of a newly isolated marine microalga, Chlamydomonas sp. JSC4, were explored. Metabolic intermediates were profiled over time to observe transient changes during the lipid accumulation triggered by the combination of the two stresses. An innovative cultivation strategy (denoted salinity-gradient operation) was also employed to markedly improve the lipid accumulation and lipid quality of the microalga, which attained an optimal lipid productivity of 223.2 mg L-1 d-1 and a lipid content of 59.4% per dry cell weight. This performance is significantly higher than reported in most related studies.ConclusionsThis work demonstrated the synergistic integration of biological and engineering technologies to develop a simple and effective strategy for the enhancement of oil production in marine microalgae.

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“…Lipids produced by microalgae can be grouped into two main categories; storage neutral lipids or non-polar lipids and structural phospholipids or polar lipids (Harwati et al 2012;Velmurugan et al 2013). Neutral lipids which are produced and accumulated during the stationary growth phase are mainly in the form of TAGs which represent a storage form of carbon and energy in the cell and can be used as an energy source (Cho et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Imaging the accumulated intracellular microalgal lipids as a response to temperature stress

et al. 2017

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Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids. This was achieved using the green alga Nannochloropsis salina. In addition, investigating the accumulation of lipid vesicles inside different isolated microalgal species as a response to temperature stress. Furthermore, the confocal laser scanning microscopy (LS510) for imaging and measuring the size and volume of the accumulated lipid vesicles was used.

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Characterization of the lipid accumulation in a tropical freshwater microalgae Chlorococcum sp.

Cited by 51 publications

References 31 publications

Growth kinetics of Chlorococcum humicola – A potential feedstock for biomass with biofuel properties

Growth kinetics of Chlorococcum humicola – A potential feedstock for biomass with biofuel properties

Optimizing biodiesel production in marine Chlamydomonassp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy

Imaging the accumulated intracellular microalgal lipids as a response to temperature stress

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