Characterization of the Male-sterile Line Bcajh97-01A/B and Identification of Candidate Genes for Genic Male Sterility in Chinese Cabbage-pak-choi

Abstract

<jats:p>Cytological features of ‘Aijiaohuang’ chinese cabbage-pak-choi (<jats:italic>Brassica campestris</jats:italic> ssp. <jats:italic>chinensis</jats:italic>) Bcajh97-01A/B genic male-sterile AB line were examined to determine phenotypic reasons for male sterility. The sterile line Bcajh97-01A was found to undergo aberrant cytokinesis during male meiosis. Transcriptional profiling of the flower buds of both fertile and sterile plants was performed at the periods preceding meiosis, at the tetrad to uninucleate pollen period, and at the binucleate to mature pollen period. Transcript-derived fragments (TDFs) from corresponding genes that were expressed in flower buds at these three different stages could be divided into nine classes. We sequenced a total of 14 new TDFs that were differentially displayed at particular pollen developmental stages, including eight genes with unknown or hypothetical functions and six genes showing significant homology with known genes. This characterization of the Bcajh97-01A genic male-sterile line allowed the identification of candidate genes underlying genic male sterility.</jats:p>

Systematic identification of long non‐coding RNAs during pollen development and fertilization in Brassica rapa

“…Cytological observations of anther development showed that the pollen mother cells are formed normally in the sterile line, but the following cycle of meiosis is disturbed and the anther fails to form tetrads. From then on, the content of the microspore begins to degenerate and consequently forms aborted mature pollen grains only coated with a defective exine wall (Huang et al ., , b). Thus, use of the ‘Bcajh97‐01A/B’ GMS A/B line is a good system to study the genes expressed during pollen development.…”

“…This makes the material a good system to study gene expression during pollen development. Differences in the expression of transcripts including protein‐coding genes and miRNAs had been revealed between fertile and sterile floral buds in our previous studies, and many candidate genes had been characterized (Huang et al ., , ,b; Jiang et al ., ; Lin et al ., , ). Here, the discovery of a set of differentially expressed lncRNAs between fertile and sterile plants provides an additional important intriguing clue to understand the regulation of male fertility in B. rapa .…”

BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

“…chinensis Makino) GMS A/B line system, which is named ‘ Bcajh97-01A/B ’. The only difference between A/B lines is that the male sterile plant (‘ Bcajh97-01A ’) undergoes an aberrant meiotic cytokinesis at the microsporogenesis stage and thus could not produce any functional mature pollen [ 42 , 43 ]. Therefore, we used the RNA-seq data of ‘ Bcajh97-01A/B ’ to identify the male fertility-related CCCH genes in B. campestris [ 44 ].…”

Genome-Wide Identification, Molecular Evolution, and Expression Profiling Analysis of Pectin Methylesterase Inhibitor Genes in Brassica campestris ssp. chinensis

“…The progenies of ‘ Bajh97 - 01A / B ’ were segregated into a homozygous male sterile line ( Bcajh97 - 01A ) and a heterozygous male fertile line ( Bcajh97 - 01B ) with a 1:1 ratio. The only difference presented between the sibling (sister) lines is that ‘ Bcajh97 - 01A ’ is in a complete absence of mature pollen compared with that of ‘ Bcajh97 - 01B ’ [ 61 , 62 ]. This feature makes ‘ Bajh97 - 01A / B ’ become an ideal material to explore the genes closely related to pollen development.…”