Characterization of the Molecular Region Containing the Active Sites of Myosin

Mueller, Helmut

doi:10.1016/s0021-9258(18)97116-4

Cited by 48 publications

(17 citation statements)

References 41 publications

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“…Sedimentation velocity studies were carried out in a Spinco Model E analytical ultracentrifuge at 59,780 rpm with the rotor temperature maintained at about 4°. For correction of the sedimentation coefficient of subfragment 1 to standard conditions, a value for 02O of 0.7420 ml/g was used (Mueller, 1965).…”

Section: Methodsmentioning

confidence: 99%

“…subfragment 1 (Mueller, 1965;Young et al, 1965). In contrast, heavy meromyosin has an intrinsic viscosity of about 0.5 dl/g (Lowey and Cohen, 1962; C. R. Zobel, unpublished data) and therefore if we assume that the subfragment 1 itself has an intrinsic viscosity of at least 0.06 dl/g then our subfragment 1 preparation could not be contaminated with more than 7 % heavy meromyosin.…”

Section: T H Imentioning

confidence: 99%

“…further tryptic digestion product of myosin, subfragment 1, was also found to retain the ATPase activity and actin-binding properties of myosin (Mueller and Perry, 1962); and since it has a molecular weight only one-third that of heavy meromyosin, several workers have suggested that a single molecule of heavy meromyosin might contain two or three identical subfragment 1 subunits (Mueller, 1965;Young et ul., 1965;Slayter and Lowey, 1967).…”

mentioning

confidence: 99%

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Subfragment 1 of myosin: adenosinetriphosphatase activation by actin

Eisenberg¹,

Cr²,

Moos³

1968

Biochemistry

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Section: Methodsmentioning

confidence: 99%

Section: T H Imentioning

confidence: 99%

mentioning

confidence: 99%

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Subfragment 1 of myosin: adenosinetriphosphatase activation by actin

Eisenberg¹,

Cr²,

Moos³

1968

Biochemistry

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“…It has been known for some time that when the globules are separated from the rod by proteolytic enzymes, both of the globular fragments are able to complex with actin. This property has, in fact, been used to isolate the globular fragments from the digestion mixture (Mueller, 1965;Jones and Perry, 1966). More recently, Young (1967a) demonstrated by ultracentrifugal transport experiments that 1 mole of the globular subfragment binds to 1 mole of F-actin monomer.…”

mentioning

confidence: 99%

Equilibrium binding of adenosine diphosphate to myosin

Lowey¹,

Luck²

1969

Biochemistry

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“…Together they isolated and named a proteolytic fraction of heavy meromyosin (itself a proteolytic product of myosin) subfragment 1 (7,8). The structural relation between subfragment 1 and heavy meromyosin was problematic, and Mueller, by then in the USA, continued to wrestle with it (Mueller 1965). It was several years before two molecules each of subfragment 1 and subfragment 2 were cleanly established as being the proteolytic product of one molecule of heavy meromyosin; this achievement was in part due to the use of papain in place of trypsin for the proteolysis (Lowey et al 1969).…”

Section: The Year In the Usa And Return To Cambridgementioning

confidence: 99%

Samuel Victor Perry. 16 July 1918 — 17 December 2009

Trentham

2011

Biogr. Mems Fell. R. Soc.

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) was a biochemist who was born in the Isle of Wight, moved shortly thereafter to King's Lynn, Norfolk, and then spent the greater part of his youth in Southport, Lancashire. His undergraduate education and early research at Liverpool University were followed by army service for the duration of World War II. After his capture in North Africa he spent much of the war as a prisoner of war, during which time his several escapes became the stuff of legends. In 1946 he began research towards his PhD degree at Cambridge University on the protein chemistry of muscle, a central theme in which he was actively engaged for more than 60 years. These were his halcyon days-member of a leading research group in muscle, alongside distinguished achievements as an English rugby international. After a Cambridge University lectureship he was appointed Head of Biochemistry in Birmingham University in 1959-a post he occupied with distinction until retirement, elevating his department to one of international stature. Among his many contributions to the protein biochemistry of muscle contraction and its regulation were the discovery of skeletal muscle myosin phosphorylation, whose significance is still a field of active research, and the recognition that the presence of the cardiac protein troponin I in the bloodstream could be used as a diagnostic marker of myocardial infarction. Perry was an inveterate gardener, especially happy in his beloved Felin Werndew, a beautiful retreat in Dinas Cross, Pembrokeshire. In August 1948 he married Maureen Shaw. She and their son and two daughters survive him. PReamBlePerry was a larger-than-life individual, whose professional and personal lives, like those of many scientists, were intimately intertwined. For this reason this memoir records his life http://dx.doi.org/10

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Characterization of the Molecular Region Containing the Active Sites of Myosin

Cited by 48 publications

References 41 publications

Subfragment 1 of myosin: adenosinetriphosphatase activation by actin

Subfragment 1 of myosin: adenosinetriphosphatase activation by actin

Equilibrium binding of adenosine diphosphate to myosin

Samuel Victor Perry. 16 July 1918 — 17 December 2009

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