Characterization of the mouse liver cell line FL83B

Breslow, Jan L.; Sloan, Howard R.; Ferrans, Víctor J.; Anderson, James L.; Levy, Robert I.

doi:10.1016/0014-4827(73)90089-x

Cited by 32 publications

(18 citation statements)

References 20 publications

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“…FL83B (CRL-2390; ATCC) is a hepatocytic cell line derived from the normal liver of a fetal mouse (37). FL83B cells were cultured in F-12K medium (ATCC) supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT).…”

Section: Methodsmentioning

confidence: 99%

FeoB-Mediated Uptake of Iron by Francisella tularensis

et al. 2013

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Francisella tularensis, the bacterial cause of tularemia, infects the liver and replicates in hepatocytes in vivo and in vitro. However, the factors that govern adaptation of F. tularensis to the intrahepatocytic niche have not been identified. Using cDNA microarrays, we determined the transcriptional profile of the live vaccine strain (LVS) of F. tularensis grown in the FL83B murine hepatocytic cell line compared to that of F. tularensis cultured in broth. The fslC gene of the fsl operon was the most highly upregulated. Deletion of fslC eliminated the ability of the LVS to produce siderophore, which is involved in uptake of ferric iron, but it did not impair its growth in hepatocytes, A549 epithelial cells, or macrophages. Therefore, we sought an alternative means by which F. tularensis might obtain iron. Deletion of feoB, which encodes a putative ferrous iron transporter, retarded replication of the LVS in iron-restricted media, reduced its growth in hepatocytic and epithelial cells, and impaired its acquisition of iron. Survival of mice infected intradermally with a lethal dose of the LVS was slightly improved by deletion of fslC but was not altered by loss of feoB. However, the ⌬feoB mutant showed diminished ability to colonize the lungs, liver, and spleen of mice that received sublethal inocula. Thus, FeoB represents a previously unidentified mechanism for uptake of iron by F. tularensis. Moreover, failure to produce a mutant strain lacking both feoB and fslC suggests that FeoB and the proteins of the fsl operon are the only major means by which F. tularensis acquires iron.

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Section: Methodsmentioning

confidence: 99%

FeoB-Mediated Uptake of Iron by Francisella tularensis

et al. 2013

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“…Subcellular Localization of Stbd1-Detection of endogenous Stbd1 by immunofluorescence in either the FL83B mouse liver cell line (39) or a Rat1 fibroblast cell line, Rat1Neo5 (28), revealed that it predominantly concentrated at perinuclear structures, with diameter up to ϳ0.5 m (Fig. 3A).…”

Section: Stdb1/genethonin 1 and Glycogen Metabolismmentioning

confidence: 99%

Starch Binding Domain-containing Protein 1/Genethonin 1 Is a Novel Participant in Glycogen Metabolism

Jiang

Heller

Tagliabracci

et al. 2010

Journal of Biological Chemistry

127

118

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Stbd1 is a protein of previously unknown function that is most prevalent in liver and muscle, the major sites for storage of the energy reserve glycogen. The protein is predicted to contain a hydrophobic N terminus and a C-terminal CBM20 glycan binding domain. Here, we show that Stbd1 binds to glycogen in vitro and that endogenous Stbd1 locates to perinuclear compartments in cultured mouse FL83B or Rat1 cells. When overexpressed in COSM9 cells, Stbd1 concentrated at enlarged perinuclear structures, co-localized with glycogen, the late endosomal/lysosomal marker LAMP1 and the autophagy protein GABARAPL1. Mutant Stbd1 lacking the N-terminal hydrophobic segment had a diffuse distribution throughout the cell. Point mutations in the CBM20 domain did not change the perinuclear localization of Stbd1, but glycogen was no longer concentrated in this compartment. Stable overexpression of glycogen synthase in Rat1WT4 cells resulted in accumulation of glycogen as massive perinuclear deposits, where a large fraction of the detectable Stbd1 co-localized. Starvation of Rat1WT4 cells for glucose resulted in dissipation of the massive glycogen stores into numerous and much smaller glycogen deposits that retained Stbd1. In vitro, in cells, and in animal models, Stbd1 consistently tracked with glycogen. We conclude that Stbd1 is involved in glycogen metabolism by binding to glycogen and anchoring it to membranes, thereby affecting its cellular localization and its intracellular trafficking to lysosomes.

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“…These differences might reflect differences in cell type. FL83B cells are derived from the mouse fetal liver (Breslow et al 1973) and YGIC5 cells from the pancreatic duct (Lee et al 2009). These cells contain the characteristics of stem cells that can differentiate into other lineages, so it would be interesting to investigate the potential of these cells to differentiate into the b-cell lineage by forcing expression of b-cellspecific transcription factors.…”

Section: Discussionmentioning

confidence: 99%

A dual-reporter system for specific tracing of pancreatic ß-cell lines that non-invasively measures viable in vivo islet cells

Kim

Choi

et al. 2009

Biotechnol Lett

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Islet transplantation is a potential treatment for type 1 diabetes. Currently, islet graft survival is measured using invasive methods to determine blood glucose, insulin, and C-peptide levels, even though these variables have limited value. To trace beta-cell survival and functional status, we constructed an adenovirus/adenoassociate virus hybrid vector (Hyb-DR) carrying two reporter genes, luciferase and green fluorescent protein (GFP), linked by the internal ribosome entry site and driven by the rat insulin II promoter. Luciferase activity increased and positive GFP expression was observed in beta-cell lines (MIN6N8 and INS-1E) infected with Hyb-DR. Using an in vivo imaging instrument, the GFP signal was detected in the flanks of nude mice 2 weeks after transplanting Hyb-DR-infected MIN6 cells into the kidney capsule. Coinfection of Hyb-DR with plasmids carrying beta-cell-specific transcription factors also resulted in expression of luciferase and GFP in the non-beta-cell lines (HepG2, FL83B, and YGIC5). Thus, the dual reporter system provided quantitative and visual information about the functionality of the islet mass and activation of the insulin gene.

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Characterization of the mouse liver cell line FL83B

Cited by 32 publications

References 20 publications

FeoB-Mediated Uptake of Iron by Francisella tularensis

FeoB-Mediated Uptake of Iron by Francisella tularensis

Starch Binding Domain-containing Protein 1/Genethonin 1 Is a Novel Participant in Glycogen Metabolism

A dual-reporter system for specific tracing of pancreatic ß-cell lines that non-invasively measures viable in vivo islet cells

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