Characterization of the mouse prostaglandin F receptor gene: a transgenic mouse study of a regulatory region that controls its expression in the stomach and kidney but not in the ovary

Hasumoto, Ken-yuh; Sugimoto, Yukihiko; Gotoh, Megumi; Segi, Eri; Yamasaki, Akira; Yamaguchi, Masahiro; Honda, Hiroaki; Hirai, Hisamaru; Negishi, Manabu; Kakizuka, Akira; Ichikawa, Atsushi

doi:10.1046/j.1365-2443.1997.1420340.x

Cited by 26 publications

(19 citation statements)

References 34 publications

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“…The human and mouse EP4 genes consist of three exons separated by two introns (Arakawa et al, 1996;Foord et al, 1996). A similar exon-intron relationship is present in the other types of prostanoid receptors, such as the DP, EP1, EP2, EP3, FP, and IP receptors (Hirata et al, 1994;Regan et al, 1994a;Batshake et al, 1995;Ogawa et al, 1995;Boie et al, 1997;Hasumoto et al, 1997;Katsuyama et al, 1998b). In the human EP4 receptor, the first exon [530 base pair (bp)] is noncoding.…”

Section: A Cloningmentioning

confidence: 99%

The Prostanoid EP4 Receptor and Its Signaling Pathway

Yokoyama

Iwatsubo²,

Umemura³

et al. 2013

Pharmacol Rev

227

257

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Section: A Cloningmentioning

confidence: 99%

The Prostanoid EP4 Receptor and Its Signaling Pathway

Yokoyama

Iwatsubo²,

Umemura³

et al. 2013

Pharmacol Rev

227

257

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“…1) is conserved among humans [12], mice [14], and cattle [15] and is similar to other prostanoid receptor genes [17]. The FPr gene consists of three exons and two introns with the translated region located in exons 2 and 3 [12,14,15]. The first exon is relatively short (160 to 194 base pairs [bp]) and includes most of the 5Ј untranslated region.…”

Section: Gene Structure and Regulation Of Fprmentioning

confidence: 99%

“…1) has been evaluated in three mammalian species: human [11,12], murine [13,14], and bovine [15]. The gene size is approximately 10 (human), 11 (mouse), and 40 (cow) kilobases (kb).…”

Section: Gene Structure and Regulation Of Fprmentioning

confidence: 99%

“…The second exon (868 to 870 bp) contains an untranslated region of approximately 70 bp and the majority of the translated receptor. The large second intron (6.1 to 33 kb) interrupts the translated region at the sixth transmembrane domain [12,14,15]. The splice junction located in the sixth transmembrane domain is also conserved in other PG receptor genes, such as the human PGE receptor-subtype 3, prostacyclin receptor and thromboxane receptor [17].…”

Section: Gene Structure and Regulation Of Fprmentioning

confidence: 99%

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Prostaglandin F2α Receptor in the Corpus Luteum: Recent Information on the Gene, Messenger Ribonucleic Acid, and Protein1

Anderson¹,

Wu²,

Tsai³

et al. 2001

Biology of Reproduction

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The prostaglandin (PG) F(2alpha) receptor (FPr) in the corpus luteum is essential for maintaining normal reproductive cyclicity in many species. Activation of this seven-transmembrane spanning receptor at the end of the cycle leads to a decrease in progesterone and the demise of the corpus luteum (luteolysis). Recently, the gene structure of the FPr in three mammalian species has been elucidated; however, promoter regulation of the gene is still poorly understood. The FPr mRNA is extremely low in steroidogenic follicular cells (theca or granulosa) but is expressed at high levels in the corpus luteum, particularly in the large luteal cells. Treatment with PGF(2alpha) decreased FPr mRNA expression in luteal cells in most species that have been studied. Key amino acids have been suggested to be critical for binding of FPr to PGF(2alpha) based on three-dimensional modeling and comparisons with other G-protein-coupled receptors. Moieties of the PGF(2alpha) molecule that are essential for binding or specificity of binding to the FPr have been identified by radioreceptor binding studies. In this article, recent information is reviewed on the structure of the FPr gene, regulation of luteal FPr mRNA, and receptor/ligand interaction requirements for the FPr protein.

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“…27,37 Although there are no published studies on the expression of FP in the human lower gastrointestinal tract, 1 previous study showed FP expression in the epithelium of the stomach. 38 We have now shown for the first time that the FP receptor is expressed in the normal colonic epithelium, and also in the epithelial component of colorectal adenomas and carcinomas. Interestingly, no significant discernible difference was observed in the intensity of FP immunoreactivity between normal and tumor samples.…”

Section: Discussionmentioning

confidence: 81%

Prostaglandin F₂_α stimulates motility and invasion in colorectal tumor cells

Qualtrough

Kaidi

Chell

et al. 2007

Intl Journal of Cancer

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Increased expression of cyclooxygenase-2 (COX-2) and subsequent prostaglandin production is an important event in several human malignancies, including colorectal cancer. COX-2 mediated prostanoid synthesis has been shown to play a key role in tumor progression with prostaglandin E 2 (PGE 2 ) being shown to promote tumor growth, invasion and angiogenesis. The role of the other prostaglandins produced by COX-2 in tumors remains poorly understood. We have shown that colorectal tumor cells produce prostaglandin F 2a (PGF 2a ) and provide evidence that PGF 2a may play an important role in colorectal tumorigenesis. Our data show that PGF 2a is secreted by both colorectal adenoma and carcinoma-derived cell lines at levels in excess of those detected for PGE 2 . These cell lines were also found to express the PGF 2a receptor (FP) indicating potential autocrine effects of PGF 2a . This finding is further supported by an in vivo immunohistochemical study of FP expression in resected colon tissue. These data show epithelial expression of FP in normal colorectal mucosa and also in colorectal adenomas and carcinomas. We compared the relative abilities of PGF 2a and PGE 2 to induce cell motility in vitro in colorectal tumor cell lines and show the first evidence of prostaglandin-induced cell motility in colorectal adenoma cell lines. PGF 2a induced cell motility with equivalent potency to PGE 2 in all the cell lines tested and was also shown to increase the invasion of carcinoma-derived cells into reconstituted basement membrane. These data show that PGF 2a may play an important role in the malignant progression of colorectal tumors. ' 2007 Wiley-Liss, Inc.

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Characterization of the mouse prostaglandin F receptor gene: a transgenic mouse study of a regulatory region that controls its expression in the stomach and kidney but not in the ovary

Cited by 26 publications

References 34 publications

The Prostanoid EP4 Receptor and Its Signaling Pathway

The Prostanoid EP4 Receptor and Its Signaling Pathway

Prostaglandin F2α Receptor in the Corpus Luteum: Recent Information on the Gene, Messenger Ribonucleic Acid, and Protein1

Prostaglandin F₂_α stimulates motility and invasion in colorectal tumor cells

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Characterization of the mouse prostaglandin F receptor gene: a transgenic mouse study of a regulatory region that controls its expression in the stomach and kidney but not in the ovary

Cited by 26 publications

References 34 publications

The Prostanoid EP4 Receptor and Its Signaling Pathway

The Prostanoid EP4 Receptor and Its Signaling Pathway

Prostaglandin F2α Receptor in the Corpus Luteum: Recent Information on the Gene, Messenger Ribonucleic Acid, and Protein1

Prostaglandin F2α stimulates motility and invasion in colorectal tumor cells

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Prostaglandin F₂_α stimulates motility and invasion in colorectal tumor cells