1985
DOI: 10.1128/mcb.5.12.3560
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Characterization of the multigene family encoding the mouse S16 ribosomal protein: strategy for distinguishing an expressed gene from its processed pseudogene counterparts by an analysis of total genomic DNA.

Abstract: Two genes from the family encoding mouse ribosomal protein S16 were cloned, sequenced, and analyzed. One gene was found to be a processed pseudogene, i.e., a nonfunctional gene presumably derived from an mRNA intermediate. The other S16 gene contained introns and had exonic sequences identical to those of a cloned S16 cDNA. The expression of this gene was demonstrated by Northern blot analysis of nuclear poly(A)+ RNA with cDNA and unique sequence intron probes. Each S16 intron contains a well-preserved remnant… Show more

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Cited by 184 publications
(117 citation statements)
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References 93 publications
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“…Although we have not ruled out the possibility that some members of the gene family are pseudogenes, it is clear that at least three members of the maize S 14 gene family are expressed. No other eukaryotic organism has been found to express more than two members of a ribosomal protein gene family, although mammalian ribosomal protein gene families often contain many pseudogenes (Dudov and Perry 1984;Wiedemann and Perry 1984;Wagner and Perry 1985).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Although we have not ruled out the possibility that some members of the gene family are pseudogenes, it is clear that at least three members of the maize S 14 gene family are expressed. No other eukaryotic organism has been found to express more than two members of a ribosomal protein gene family, although mammalian ribosomal protein gene families often contain many pseudogenes (Dudov and Perry 1984;Wiedemann and Perry 1984;Wagner and Perry 1985).…”
Section: Discussionmentioning
confidence: 99%
“…Because many mammalian ribosomal protein gene families contain large numbers (7-20) of pseudogenes and only a single expressed copy (Dudov and Perry 1984;Wiedemann and Perry 1984;Wagner and Perry 1985), we were interested in estimating how many S 14 gene copies were expressed in maize. Nine additional cDNA clones were isolated from a eDNA library constructed from 8-day postpollination endosperm RNA of the A188 inbred line (j. Hunsperger, D. Culley, I. Rubentein, and M. Brenner, unpubl.).…”
Section: The Maize S14 Ribosomal Protein Is Encoded By a Small Multigmentioning
confidence: 99%
“…As we know that these genes are very conserved throughout species, the existence of such a global regulator or common motif of regulation probably exists in the rat genome. The overexpression of several ribosomal protein genes can be compared to the upregulation of TCTP, another gene isolated in the course of this differential screening, since this gene shares a feature observed on the 5' terminus of the transcripts of some mouse ribosomal protein genes, a run of Ts followed by a GC-rich segment (Chitpatima et al, 1988;Wagner and Perry 1985). This finding raises the possibility that this conserved sequence could be a target for a transcriptional factor induced during this cell death program.…”
Section: Ribosomal Protein and Translationally Controlled Tumor Protementioning
confidence: 99%
“…For this purpose we selected three mouse ribosomal protein genes, rpLSO, rpL32, and rpS16. These single-copy genes are located on different chromosomes (Wiedemann et al 1987) and are evolutionarily unrelated, as judged from the complete dissimilarity of their sequences (Dudov and Perry 1984;Wiedemann and Perry 1984;Wagner and Perry 1985). In this paper, we present a detailed analysis of the rpLSO promoter and compare its properties with those of rpL32 (Dudov and Perry 1986;Atchison et al 1989;Chung and Perry 1989;Moura-Neto et al 1989) and rpS16 (Hariharan and Perry 1989).…”
mentioning
confidence: 97%