Characterization of the Mycovirome from the Plant-Pathogenic Fungus Cercospora beticola

Li, Yingxi; Zhou, M.; Yang, Yi‐Zhou; Liu, Qi; Zhang, Zongying; Han, Chenggui; Wang, Ying

doi:10.3390/v13101915

Cited by 9 publications

(3 citation statements)

References 59 publications

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“…Members of this family have been reported as fungal viruses or mycoviruses, being found in a variety of fungal species associated with asymptomatic infections (Ghabrial and Suzuki, 2008). Additionally, they have been found in pathogenic fungi, such as Rhizoctonia solani, Magnaporthe oryzae, Cercospora beticola (Abdoulaye et al, 2019;Liu et al, 2020;Li et al, 2021) and the ectomycorrhizal fungus Geopora sumneriana (Sahin and Akata, 2019).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

et al. 2023

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IntroductionTheobroma cacao, the cocoa tree, is a target for pathogens, such as fungi from the genera Phytophthora, Moniliophthora, Colletotrichum, Ceratocystis, among others. Some cacao pathogens are restricted to specific regions of the world, such as the Cacao swollen shoot virus (CSSV) in West African countries, while others are expanding geographically, such as Moniliophthora roreri in the Americas. M. roreri is one of the most threatening cacao pathogens since it directly attacks the cacao pods driving a significant reduction in production, and therefore economic losses. Despite its importance, the knowledge about the microenvironment of this pathogen and the cocoa pods is still poorly characterized.MethodsHerein we performed RNA sequencing of spores in differential stages of culture in a medium supplemented with cacao pod extract and mycelium collected of the susceptible variety ICT 7121 naturally infected by the pathogen to evaluate the diversity and transcriptional activity of microorganisms associated with the in vitro sporulation of M. roreri.ResultsOur data revealed a great variety of fungi and bacteria associated with M. roreri, with an exceptional diversity of individuals from the genus Trichoderma sp. Interestingly, the dynamics of microorganisms from different kingdoms varied proportionally, suggesting they are somehow affected by M. roreri culture time. We also identified three sequences similar to viral genomes from the Narnaviridae family, posteriorly confirmed by phylogenetic analysis as members of the genus Narnavirus. Screening of M. roreri public datasets indicated the virus sequences circulating in samples from Ecuador, suggesting a wide spread of these elements. Of note, we did not identify traces of the viral sequences in the M. roreri genome or DNA sequencing, restricting the possibility of these sequences representing endogenized elements.DiscussionTo the best of our knowledge, this is the first report of viruses infecting the fungus of the genus Moniliophthora and only the third description of viruses that are able to parasite elements from the Marasmiaceae family.

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Section: Discussionmentioning

confidence: 99%

Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

et al. 2023

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“…Recently, deep sequencing has been used to identify the diversity of fungal viruses within fungal species from diverse geographic regions [52,55,70,71,82,[84][85][86][87][88][89][90]. Unlike other dsRNA extraction and cloning methods, this approach could obtain viral information for different classified viruses regardless of their genome types [13,14,56,86].…”

Section: Discussionmentioning

confidence: 99%

Novel Mycoviruses Discovered from a Metatranscriptomics Survey of the Phytopathogenic Alternaria Fungus

Wang

et al. 2022

Viruses

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Alternaria fungus can cause notable diseases in cereals, ornamental plants, vegetables, and fruits around the world. To date, an increasing number of mycoviruses have been accurately and successfully identified in this fungus. In this study, we discovered mycoviruses from 78 strains in 6 species of the genus Alternaria, which were collected from 10 pear production areas using high-throughput sequencing technology. Using the total RNA-seq, we detected the RNA-dependent RNA polymerase of 19 potential viruses and the coat protein of two potential viruses. We successfully confirmed these viruses using reverse transcription polymerase chain reaction with RNA as the template. We identified 12 mycoviruses that were positive-sense single-stranded RNA (+ssRNA) viruses, 5 double-strand RNA (dsRNA) viruses, and 4 negative single-stranded RNA (−ssRNA) viruses. In these viruses, five +ssRNA and four −ssRNA viruses were novel mycoviruses classified into diverse the families Botourmiaviridae, Deltaflexivirus, Mymonaviridea, and Discoviridae. We identified a novel −ssRNA mycovirus isolated from an A. tenuissima strain HB-15 as Alternaria tenuissima negative-stranded RNA virus 2 (AtNSRV2). Additionally, we characterized a novel +ssRNA mycovirus isolated from an A. tenuissima strain SC-8 as Alternaria tenuissima deltaflexivirus 1 (AtDFV1). According to phylogenetic and sequence analyses, we determined that AtNSRV2 was related to the viruses of the genus Sclerotimonavirus in the family Mymonaviridae. We also found that AtDFV1 was related to the virus family Deltaflexivirus. This study is the first to use total RNA sequencing to characterize viruses in Alternaria spp. These results expand the number of Alternaria viruses and demonstrate the diversity of these mycoviruses.

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“…Viruses with genomic similarities to bunyaviruses have more recently also been described from fungi and oomycetes. Fugal species hosting these unclassified bunya-like viruses include several potential phytopathogens [38][39][40][41][42] as well as plant endophytes [43]. Further hosts include the shiitake mushroom (Lentinula edodes) [44] and a marine fungus, Penicillium roseopurpureum [45].…”

Section: Introductionmentioning

confidence: 99%

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Poimala

Raco

Haikonen

et al. 2022

Viruses

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Phytophthora cactorum is an important oomycetous plant pathogen with numerous host plant species, including garden strawberry (Fragaria × ananassa) and silver birch (Betula pendula). P. cactorum also hosts mycoviruses, but their phenotypic effects on the host oomycete have not been studied earlier. In the present study, we tested polyethylene glycol (PEG)-induced water stress for virus curing and created an isogenic virus-free isolate for testing viral effects in pair with the original isolate. Phytophthora cactorum bunya-like viruses 1 and 2 (PcBV1 & 2) significantly reduced hyphal growth of the P. cactorum host isolate, as well as sporangia production and size. Transcriptomic and proteomic analyses revealed an increase in the production of elicitins due to bunyavirus infection. However, the presence of bunyaviruses did not seem to alter the pathogenicity of P. cactorum. Virus transmission through anastomosis was unsuccessful in vitro.

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Characterization of the Mycovirome from the Plant-Pathogenic Fungus Cercospora beticola

Cited by 9 publications

References 59 publications

Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

Novel Mycoviruses Discovered from a Metatranscriptomics Survey of the Phytopathogenic Alternaria Fungus

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

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