Characterization of the natural peptidome of four leeches by integrated proteogenomics and pseudotargeted peptidomics

Efficacy and potential mechanism of atherosclerosis prevention by the active components of leech based on network pharmacology combined with animal experiments

“…A database of leech active ingredients was established by referring to the literature on leech chemical composition [ [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] ],the SMILES structure was obtained by searching the leech active constituent PubChem database [ 26 ] ( https://pubchem.ncbi.nlm.nih.gov/ ).…”

Evaluation of quality consistency between dispensing granules and traditional decoction of Bombyx batryticatus based on peptidomics and in silico simulations

“…Peptidomics is a method for analyzing the composition and characteristics of endogenous peptides in biological tissues or cells, typically achieved through liquid chromatography (LC) separation combined with mass spectrometry (MS) detection (Hellinger et al, 2023). The utilization of peptidomics has been widespread in identifying various peptides found in animal-derived TCMs, such as Pheretima (Luo et al, 2023), Bubali Cornu (Liu et al, 2017), and leech (Liao et al, 2023). In addition, in the drug development phase, virtual screening serves as an efficient and convenient computational simulation method.…”