Characterization of the nuclear import pathway for BLM protein

Duan, Zhiqiang; Zhao, Jianjian; Xu, Houqiang; Xu, Haixu; Ji, Xinqin; Chen, Xiang; Xiong, Jianming

doi:10.1016/j.abb.2017.09.019

Cited by 4 publications

(3 citation statements)

References 63 publications

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“… 28 NUTF2 depletion by siRNA disrupts the accumulation of BLM protein in the nucleus. 29 Previous studies have shown that the diagnosis and staging of cancer is based on the hierarchical growth of nuclear size, 30 and it has been reported that RAN-recycling function of NUTF2 is involved in the regulation of nuclear size. 5 The expression of NUTF2 thus may participate in the alteration of the nuclear size in cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of NUTF2 as a Candidate Diagnostic and Prognostic Biomarker Associated with Immune Infiltration in Head and Neck Squamous Cell Carcinoma

Zhang¹,

Gao²

2021

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Background: Head and neck squamous cell carcinoma (HNSC) is one of the most common tumors worldwide. Nuclear transport factor 2 (NUTF2) plays a key role in cell death and immune processes. However, few reports have studied correlations between NUTF2 gene expression and the occurrence and development of HNSC. Methods: The expression of NUTF2 was analyzed using publicly available databases, including the Cancer Genome Atlas and Human Protein Atlas and Gene Expression Omnibus (GEO) database, which was validated by RT-PCR. We evaluated the functions of NUTF2 with Kaplan-Meier curve, logistic regression were used to study the relationship between clinicopathological features and the expression of NUTF2. Cox regression analyses were used to identify the effects of NUTF2 expression on survival. Gene Ontology and Gene Set Enrichment Analysis were used to explore relevant biological pathways. The relationship between NUTF2 and tumor-infiltrating immune cells was investigated with on-line bioinformatic tools. Results: NUTF2 was significantly upregulated in HNSC lesions and is associated with tumor size (P < 0.01). Increased expression of NUTF2 was linked to shorter overall and progress-free survival in HNSC. Cox regression analyses revealed that NUTF2 is an independent prognostic factor in HNSC. GSEA analysis demonstrated that NUTF2 negatively regulates several immune pathways. NUTF2 was correlated with the infiltrating levels of B cells and CD8+ T cells and was negatively correlated with diverse immune marker sets in HNSC. Conclusion: NUTF2 is highly expressed in HNSC and correlates with poor prognosis. Correlation with immune functions suggests that NUTF2 may serve as a biomarker and therapeutic target for HNSC.

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Section: Discussionmentioning

confidence: 99%

Identification of NUTF2 as a Candidate Diagnostic and Prognostic Biomarker Associated with Immune Infiltration in Head and Neck Squamous Cell Carcinoma

Zhang¹,

Gao²

2021

OTT

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“…Generally, classical NLSs including monopartite and bipartite NLSs are transported into the nucleus by importin α/β heterodimer, whereas non-classical NLSs can be more complex in sequence, length and amino acid composition that are imported by importin β [ 22 , 24 ]. However, large numbers of studies have found that classical NLSs can also be recognized and bound by importin β1, such as the NLS of HTLV-1 Rex (RRRPRRSQRKR) [ 64 ], HIV-1 Tat (RKKRRQRRR) and Rev (RQARRNRRRR) [ 65 ], Smad3 (KKLKK) [ 66 ], TopBP1 (RKRK) [ 67 ], and BLM (RSKRRK) [ 68 ]. It is reported that NDV M protein localizes to the nucleus via a bipartite NLS (KKGKKVIFDKIEEKIRR) [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have confirmed that the HEAT repeats of importin β1 can provide abundant binding regions for interaction with distinct cargo proteins. For example, the cellular proteins PTHrP [ 73 ], Snail [ 74 ], BLM [ 68 ], SREBP2 [ 75 ] and TopBP1 [ 67 ] interact with importin β1 by binding the 2–11, 5–14, 14–16, 7–17 and 18–19 HEAT repeats of importin β1, respectively. Although the HEAT repeats used to bind three cargo proteins (2–11 for PTHrP, 5–14 for Snail, and 7–17 for SREBP2) is overlapped, the binding mechanism for each protein is distinctly different [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Importin α5 negatively regulates importin β1-mediated nuclear import of Newcastle disease virus matrix protein and viral replication and pathogenicity in chicken fibroblasts

Duan

et al. 2018

Virulence

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The matrix (M) protein of Newcastle disease virus (NDV) is demonstrated to localize in the nucleus via intrinsic nuclear localization signal (NLS), but cellular proteins involved in the nuclear import of NDV M protein and the role of M's nuclear localization in the replication and pathogenicity of NDV remain unclear. In this study, importin β1 was screened to interact with NDV M protein by yeast two-hybrid screening. This interaction was subsequently confirmed by co-immunoprecipitation and pull-down assays. In vitro binding studies indicated that the NLS region of M protein and the amino acids 336–433 of importin β1 that belonged to the RanGTP binding region were important for binding. Importantly, a recombinant virus with M/NLS mutation resulted in a pathotype change of NDV and attenuated viral replication and pathogenicity in chicken fibroblasts and SPF chickens. In agreement with the binding data, nuclear import of NDV M protein in digitonin-permeabilized HeLa cells required both importin β1 and RanGTP. Interestingly, importin α5 was verified to interact with M protein through binding importin β1. However, importin β1 or importin α5 depletion by siRNA resulted in different results, which showed the obviously cytoplasmic or nuclear accumulation of M protein and the remarkably decreased or increased replication ability and pathogenicity of NDV in chicken fibroblasts, respectively. Our findings therefore demonstrate for the first time the nuclear import mechanism of NDV M protein and the negative regulation role of importin α5 in importin β1-mediated nuclear import of M protein and the replication and pathogenicity of a paramyxovirus.

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Multifunctionality of matrix protein in the replication and pathogenesis of Newcastle disease virus: A review

Duan,

Zhang,

Liu

et al. 2023

International Journal of Biological Macromolecules

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Characterization of the nuclear import pathway for BLM protein

Cited by 4 publications

References 63 publications

Identification of NUTF2 as a Candidate Diagnostic and Prognostic Biomarker Associated with Immune Infiltration in Head and Neck Squamous Cell Carcinoma

Identification of NUTF2 as a Candidate Diagnostic and Prognostic Biomarker Associated with Immune Infiltration in Head and Neck Squamous Cell Carcinoma

Importin α5 negatively regulates importin β1-mediated nuclear import of Newcastle disease virus matrix protein and viral replication and pathogenicity in chicken fibroblasts

Multifunctionality of matrix protein in the replication and pathogenesis of Newcastle disease virus: A review

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