Characterization of the nuclear import and export signals of pseudorabies virus UL31

Li, Meili; Jiang, Siwen; Wang, Jinlin; Mo, Chuncong; Zeng, Zhan-Cheng; Yang, Yanjia; Chen, Chunke; Li, Xiaowei; Cui, Wei; Huang, Jinlu; Peng, Tao; Cai, Mingsheng

doi:10.1007/s00705-015-2527-7

Cited by 21 publications

(17 citation statements)

References 19 publications

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“…Moreover, PRV UL31-EYFP, pEYFP-BFLF2, pUL4-EYFP, pTAP-mCherry, pCRM1-mCherry, pDN kα1-mCherry, pDN kβ1-mCherry, pRan-Q69L-mCherry and pFlag-importin β2 expression plasmids were also constructed in our lab previously [23,25,31,60,61]. Plasmid pNucleolin-EGFP, a nucleolar marker, was provided by Dr.…”

Section: Construction Of Expression Plasmidsmentioning

confidence: 99%

Nuclear Import of Epstein-Barr virus BLLF2 is Mediated by an Importin β1-Dependent Mechanism

Guo

Deng

et al. 2020

Preprint

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Background: Epstein-Barr virus (EBV), the pathogen of several human malignancies, encodes many proteins that require to be transported into the nucleus for viral DNA reproduction and nucleocapsids assembly in the lytic replication cycle. A nuclear membrane phosphoprotein encoded by EBV BLLF2, is believed to associate with viral DNA packaging and primary egress across the nuclear membrane. Results: Here, fluorescence microscope, mutation analysis, interspecies heterokaryon assays, co-immunoprecipitation assays and western blot were performed to explore the nuclear import mechanism of BLLF2. As results, BLLF2 was shown to be a nucleocytoplasmic shuttling protein, which was mediated neither by chromosomal region maintenance 1 (CRM1)- nor transporter associated with antigen processing (TAP)-dependent pathway. Yet, two functional nuclear localization signals (NLSs) of BLLF2, NLS1 (16KRQALETVPHPQNRGR31) and NLS2 (48PPVAKRRR58), were identified, whereas the predicted NES was nonfunctional. Finally, BLLF2 was proved to transport into the nucleus via Ran-dependent and importin β1-dependent pathway. Conclusions: This mechanism may contribute to a more extensive insight of the assembly and synthesis of EB virions in the nucleus, thus affording a new direction for the treatment of viruses.

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Section: Construction Of Expression Plasmidsmentioning

confidence: 99%

Nuclear Import of Epstein-Barr virus BLLF2 is Mediated by an Importin β1-Dependent Mechanism

Guo

Deng

et al. 2020

Preprint

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“…2). UL31 homologs have nuclear localization signals (Funk et al, 2015; Li et al, 2015; Passvogel et al, 2015), and in the absence of UL34, they are diffusely distributed throughout the nucleoplasm (Zhu et al, 1999). Although some UL34 homologs have predicted nuclear localization signals, most do not.…”

Section: The Role Of Nec In Membrane Buddingmentioning

confidence: 99%

Have NEC Coat, Will Travel

Bigalke

Heldwein

2017

Advances in Virus Research

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Herpesviruses are unusual among enveloped viruses because they bud twice yet acquire a single envelope. Furthermore, unlike other DNA viruses that replicate in the nucleus, herpesviruses do not exit it by passing through the nuclear pores or by rupturing the nuclear envelope. Instead, herpesviruses have a complex mechanism of nuclear escape whereby nascent capsids bud at the inner nuclear membrane to form perinuclear virions that subsequently fuse with the outer nuclear membrane, releasing capsids into the cytosol. This makes them some of the very few known viruses that bud into the nuclear envelope. The envelope acquired during nuclear budding does not end up in the mature viral particle but instead allows the capsid to translocate from the nucleus into the cytosol. The viral nuclear egress complex (NEC) is a critical player in the nuclear egress, yet its function and mechanism have remained enigmatic. Recent studies have demonstrated that the NEC buds membranes without the help of other proteins by forming a honeycomb coat, which established the NEC as the first virally encoded budding machine that operates at the nuclear, as opposed to cytoplasmic, membrane. This review discusses our current understanding of the NEC budding mechanism, with the emphasis on studies that illuminated the structure of the NEC coat and its role in capsid budding during herpesvirus nuclear escape.

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“…Transfection and fluorescence microscopy procedures were carried out as reported in our previous researches (6)(7)(8)(13)(14)(15). For duplicate the assay, each transfection was manipulated at least three times.…”

Section: Plasmid Transfection and Fluorescence Microscopymentioning

confidence: 99%

Effects of Heat Shock and 2, 4-D Treatment on Morphological and Physiological Characteristics of Microspores and Microspore-Derived Doubled Haploid Plants in Brassica napus L.

et al. 2015

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Characterization of the nuclear import and export signals of pseudorabies virus UL31

Cited by 21 publications

References 19 publications

Nuclear Import of Epstein-Barr virus BLLF2 is Mediated by an Importin β1-Dependent Mechanism

Nuclear Import of Epstein-Barr virus BLLF2 is Mediated by an Importin β1-Dependent Mechanism

Have NEC Coat, Will Travel

Effects of Heat Shock and 2, 4-D Treatment on Morphological and Physiological Characteristics of Microspores and Microspore-Derived Doubled Haploid Plants in Brassica napus L.

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