Characterization of the Oxidative Enzyme Potential in Wild White Rot Fungi From Misiones (Argentina)

Fonseca, María Isabel; Zapata, Pedro Darío; Villalba, Laura Lidia; Fariña, Julia Inés

doi:10.15446/abc.v20n1.38322

Cited by 9 publications

(7 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Almost all species of WRF were reported to produce laccase to a varying degree (Hatakka 1994). After screening several WRF strains, P. sajor-caju evidenced laccase activity and also showed high phenol oxidation rates, indicating the significance of Note: The half-life of laccase activity is expressed as the time necessary for the enzymatic activity to be reduced by 50 % additional approach to evaluate a potential biotechnological application (Fonseca et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In parallel, using the same experimental conditions, laccase activity was revealed for which the plate was covered with 1.2 mM ABTS [2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)] in acetate buffer pH 4.5 and incubated in dark for 20 min; on the other hand, it was also revealed with 5 mM of DMP in the same buffer. The appearance of green and orange colors in the solid media, respectively, indicated a positive result (Fonseca et al 2015). At the same time, another assay was carried out to determine the laccase specific activity in plates with effluents.…”

Section: Growth Assay and Laccase Secretion On Solid Culture With Colmentioning

confidence: 99%

“…This process is due to the fact that they have an oxidative enzymatic system, a group of extracellular ligninolytic enzymes (Fonseca et al 2010). They usually involve enzymes such as lignin peroxidase (LiP, EC 1.11.1.14), able to oxidize directly non-phenolic units, whilst manganese peroxidase (MnP, EC 1.11.1.13) and laccase (Lac, EC 1.10.3.2) preferentially oxidize phenolic compounds, although non-phenolic units may eventually be degraded in presence of mediators (Fonseca et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…This basidiomycete secretes a range of enzymes, most notably laccases, enabled to grow on a variety of different substrates. In Misiones (Argentina), several WRF such us Pleurotus sajor-caju with laccase activity, have been described leading to interesting possibilities for biotechnology as for example in the Kraft liquor decolorization ability on lignin-rich effluents (Fonseca et al 2015).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

Fonseca

Molina

Benítez

et al. 2020

Rev. Int. Contam. Ambie.

View full text Add to dashboard Cite

This research is aimed to evaluate the effect of copper on biomass growth, laccase activity and isoenzymes composition of Pleurotus sajor-caju, and its ability to grow on effluents of the citrus industry. The inhibitory effect of copper on growth was evidenced especially after 14 days of culture in the presence of the highest concentration of copper (1 mM) reducing almost three times its growth. The highest enzyme activity was reached at the 10th day of treatment, with ~ 53 U/L for the medium without copper and ~ 121 and ~ 68 U/L for medium supplemented with 0.5 and 1mM, respectively. Laccases in cell-free extracts obtained from culture added with copper showed to be more stable for more time at temperature changes. The optimum temperature for laccase activity increased from 50 to 60 ºC by effect of copper while the optimal pH was 5 in all experiments. In gels, it was possible to observe two isoenzymes of 65 and 35 kDa whose expression varied according to incubation time. Moreover, the isoenzyme of low molecular weight was induced by the presence of effluent in solid medium. P. sajor-caju had the ability to grow on effluents from the citrus industry, showing tolerance and potential for waste treatment, constituting a possible alternative to biodegrade and reduce the contaminating impact of effluents. Palabras clave: biotecnología, catalizadores verdes, hongo de pudrición blanca RESUMEN Esta investigación tuvo como objetivo evaluar el efecto del cobre en el crecimiento de la biomasa, la actividad de lacasas y la composición de isoenzimas de Pleurotus sajorcaju, así como su capacidad para crecer en los efluentes de la industria citrícola. El efecto inhibidor del cobre sobre el crecimiento se evidenció especialmente después de 14 días de cultivo en presencia de la mayor concentración de cobre (1 mM), reduciendo casi tres veces el crecimiento. La actividad enzimática más alta se alcanzó a los 10 días para todos los tratamientos, con ~ 53 U/L para el medio sin cobre y ~

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Growth Assay and Laccase Secretion On Solid Culture With Colmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

Fonseca

Molina

Benítez

et al. 2020

Rev. Int. Contam. Ambie.

View full text Add to dashboard Cite

show abstract

“…It has been reported that copper acts at the pre-transcriptional level regulating the initiation of the laccase production. 42 The positive effects of CuSO 4 on laccase production by Bacillus sp. PK4 were also previously observed by Rajeswari and Bhuvaneswari.…”

Section: Fermentation Of Sb For Laccase Productionmentioning

confidence: 99%

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Abbas,

Ejaz,

Sohail

et al. 2024

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Sugarcane bagasse (SB) is an abundantly available agrowaste that is used extensively as a substrate for the production of different industrial enzymes. It is a renewable resource, rich in fermentable sugars. However, the presence of lignin makes it difficult for microbes to degrade it and so it is difficult to utilize it. Keeping in mind the role of white rot fungi in lignin degradation, Trametes pubescens MB89 was used in this study for the delignification of SB with the simultaneous production of laccase. The fungus was also used for the degradation of malachite green dye. A Box–Behnken design (BBD) was used to optimize variables affecting solid state fermentation of SB for laccase production. The leftover SB after fermentation was used for cellulase production by the thermophilic bacterium Neobacillus sedimentimangrovi UE25. The BBD depicted optimized conditions as 60% moisture, particle size 100 μ, 10 days’ incubation period, 450 μM CuSO4, and a temperature of 30 °C. The laccase titers of 1271 IU mL−1 were obtained under optimized conditions. Neobacillus sedimentimangrovi UE25 produced 4.574 IU mL−1 of endoglucanase and 1.812 IU mL−1 of β‐glucosidase by utilizing fungal pretreated SB. Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed the impact of fungal cultivation on SB. Pores were observed after microbial pretreatment and there were changes in the morphological characteristics such as the pith and fiber of SB. To test the ability of laccase to break down aromatic dyes, we grew the fungus T. pubescens MB89 in a nutrient‐rich medium supplemented with malachite green dye. The fungus was able to remove 73.68% of the malachite green dye from the medium in 24 h. This shows that T. pubescens MB89 has a range of potential applications for environmental and industrial purposes.

show abstract

Bioconversion and Biotransformation Efficiencies of Wild Macrofungi

et al. 2018

View full text Add to dashboard Cite

Characterization of the Oxidative Enzyme Potential in Wild White Rot Fungi From Misiones (Argentina)

Cited by 9 publications

References 32 publications

COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

COPPER IMPROVES THE PRODUCTION OF LACCASE BY Pleurotus sajor-caju WITH ABILITY TO GROW ON EFFLUENTS OF THE CITRUS INDUSTRY

Application of Trametes pubescens for dye removal and biological pretreatment of sugarcane bagasse

Bioconversion and Biotransformation Efficiencies of Wild Macrofungi

Contact Info

Product

Resources

About