Characterization of the Phosphofructokinase Gene Family in Rice and Its Expression Under Oxygen Deficiency Stress

Mustroph, Angelika; Stock, Johanna; Hess, Natalia; Aldous, Sophia H.; Dreilich, Anika; Grimm, Bernhard

doi:10.3389/fpls.2013.00125

Cited by 66 publications

(44 citation statements)

References 78 publications

(126 reference statements)

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“…In "Granny Smith" apples, PFK is highly responsive to subtle changes in oxygen concentration (Cukrov et al, 2016), and, similarly to SuSy, uses PPi instead of ATP to save energy (Bailey-Serres et al, 2012). PFK most likely represents a key element for re-setting carbon metabolism under oxygen deficiency through an induction of the glycolytic pathway in rice (Mustroph et al, 2013). Both PFK and pyruvate kinase (PK) are highly and rapidly responsive to subtle changes of oxygen concentrations in apple tissues (Brizzolara et al, 2019).…”

Section: Carbohydrate Metabolismmentioning

confidence: 99%

Primary Metabolism in Fresh Fruits During Storage

et al. 2020

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The extension of commercial life and the reduction of postharvest losses of perishable fruits is mainly based on storage at low temperatures alone or in combination with modified atmospheres (MAs) and controlled atmospheres (CAs), directed primarily at reducing their overall metabolism thus delaying ripening and senescence. Fruits react to postharvest conditions with desirable changes if appropriate protocols are applied, but otherwise can develop negative and unacceptable traits due to the onset of physiological disorders. Extended cold storage periods and/or inappropriate temperatures can result in development of chilling injuries (CIs). The etiology, incidence, and severity of such symptoms vary even within cultivars of the same species, indicating the genotype significance. Carbohydrates and amino acids have protective/regulating roles in CI development. MA/CA storage protocols involve storage under hypoxic conditions and high carbon dioxide concentrations that can maximize quality over extended storage periods but are also affected by the cultivar, exposure time, and storage temperatures. Pyruvate metabolism is highly reactive to changes in oxygen concentration and is greatly affected by the shift from aerobic to anaerobic metabolism. Ethylene-induced changes in fruits can also have deleterious effects under cold storage and MA/CA conditions, affecting susceptibility to chilling and carbon dioxide injuries. The availability of the inhibitor of ethylene perception 1-methylcyclopropene (1-MCP) has not only resulted in development of a new technology but has also been used to increase understanding of the role of ethylene in ripening of both non-climacteric and climacteric fruits. Temperature, MA/CA, and 1-MCP alter fruit physiology and biochemistry, resulting in compositional changes in carbon-and nitrogen-related metabolisms and compounds. Successful application of these storage technologies to fruits must consider their effects on the metabolism of carbohydrates, organic acids, amino acids and lipids.

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Section: Carbohydrate Metabolismmentioning

confidence: 99%

Primary Metabolism in Fresh Fruits During Storage

et al. 2020

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“…The hexokinase (HK) activity was assayed according to Schaffer and Petreikov [75]. The phosphofructokinase (PFK) activity was measured according to Mustroph et al [76]. The pyruvate kinase (PK) was determined according to Schweizer and Erismann [77].…”

Section: Transcriptome Assembly and Gene Functional Annotationmentioning

confidence: 99%

Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress

Dong

Jiang

et al. 2020

Preprint

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Background Low temperature limits the growth and geographical distribution of plants. Poa pratensis is a cool-season turfgrass mainly grown in urban areas. However, low winter temperature or cold events in spring and autumn may cause P.pratensis mortality, affecting the appearance of lawns. P.pratensis var. anceps cv. Qinghai (PQ) is widely distributed in the Qinghai-Tibet Plateau above 3000 m. PQ has greater cold resistance than the commercially cultivated P.pratensis varieties. However, existing studies on the response mechanism of PQ to low temperatures have mainly focused on physiological and biochemical perspectives, while changes in the PQ transcriptome during the response to cold stress have not been reported. Results To investigate the molecular mechanism of the PQ cold response and identify genes to improve the low-temperature resistance of P.pratensis, we analyzed and compared the transcriptomes of PQ and the cold-sensitive P.pratensis cv. ‘Baron’ (PB) under cold stress using RNA sequencing. We identified 4878 and 1871 differentially expressed genes (DEGs) between the treatment vs control comparison of PQ and PB, respectively, with 4494 DEGs specific to PQ. Based on the DEGs, important Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as “starch and sucrose metabolism”, “protein processing in endoplasmic reticulum”, “phenylalanine metabolism” and “glycolysis/gluconeogenesis” were significantly enriched in PQ, and “starch and sucrose metabolism”, “phenylpropanoid biosynthesis”, “galactose metabolism” and “glutathione metabolism” were significantly enriched in PB. In addition, the “glycolysis” and “citrate cycle (TCA cycle)” pathways were identified as involved in cold resistance of P.pratensis. Conclusions As we know, this is the first study to explore the transcriptome of P.pratensis var. anceps cv. Qinghai. Our study not noly provides important insights into the molecular mechanisms of P.pratensis var. anceps cv. Qinghai responds to cold stress, but also systematically reveals the changes of key genes and products of glycolysis and TCA cycle in response to cold stress, which is conductive to the breeding of cold-resistant P.pratensis genotype.

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“…ATP-PFKs are found to be a large gene family: at least seven genes encoding ATP-PFK were found in Arabidopsis (Mustroph et al 2007) and ten were found from rice (Mustroph et al 2013). PFP genes are of a smaller gene family.…”

Section: S Gfpmentioning

confidence: 99%

Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

Qin

Kaas

Wu³

et al. 2014

Tree Genetics & Genomes

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Pyrophosphate-dependent phosphofructokinase (PFP) catalyses the reversible phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate in the glycolysis pathway. Two full-length complementary DNAs encoding putative PFP α-and β-subunits, named EjPFPa and EjPFPb1, were isolated by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE) from Eriobotrya japonica Lindl. (loquat). The other β-subunit gene was identified from transcriptome data obtained by Illumina sequencing, designated as EjPFPb2. They share up to 88 % identity with other plant PFP α/β-subunits. EjPFPa and EjPFPb clustered separately in clades of plant PFP α-or β-subunits, respectively. EjPFPa and EjPFPb were both shown to be mainly localised in the cell membrane using confocal microscopy of GFP fusion proteins 35S:EjPFPa/b-GFP. Monitoring the dynamic changes of transcripts and proteins demonstrated that EjPFPa and EjPFPb did not show coordinated expression during fruit development in loquat. The transcript levels of EjPFPa and EjPFPb in the leaves of loquat seedlings were significantly enhanced after 3 h of treatment with 0.5, 1.0 and 1.5 M fructose or glucose, which indicates that EjPFPa and EjPFPb are modulated by fructose and glucose in vivo. Transgenic tobacco plants overexpressed EjPFPa did not show visible phenotype changes, while plants overexpressing EjPFPb1 grew faster at the cost of reduced leaf size and sucrose content. Meanwhile, fructose content increased in growing plants compared with wild-type plants.The results confirm that genes of the two subunits from loquat PFP have different transcriptional regulation systems and play different roles in carbohydrate metabolism. Elevation of the PFP β-subunit gene expression can partly impact the glycolytic carbon allocation in growing plants.

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Characterization of the Phosphofructokinase Gene Family in Rice and Its Expression Under Oxygen Deficiency Stress

Cited by 66 publications

References 78 publications

Primary Metabolism in Fresh Fruits During Storage

Primary Metabolism in Fresh Fruits During Storage

Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress

Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

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