Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

Chantha, Sier-Ching; Emerald, Bright Starling; Matton, Daniel P.

doi:10.1007/s11103-006-9064-4

Cited by 18 publications

(27 citation statements)

References 69 publications

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“…This discrepancy could be explained by RNA interference showing different levels of effectiveness on the NLE gene in the sporophytic tissues of these two plant species. While clear reduction in ScNLE transcript levels was detected in S. chacoense transformants (Chantha et al, 2006), this was not the case for AtNLE transcripts in Arabidopsis transformants. Another explanation could be that Arabidopsis sporophytic development is more sensitive to changes in AtNLE expression levels and could not survive to an important reduction in transcript levels.…”

Section: Discussionmentioning

confidence: 70%

“…Moreover, the semisterility phenotype was maintained in the following generations. Previous studies reported that RNA interference is not always associated to detectable reduction in targeted gene transcript levels (Kerschen et al, 2004) and that levels of residual targeted gene transcript are not necessarily correlated to degree of phenotype severity (Acosta-Garcia and Chantha et al, 2006). In cleared ovules of the AtNLE-RNAi lines analyzed, female gametophytes in proportions corresponding to levels of aborted ovules were either degenerated or arrested at early developmental stages, including as early as during the megaspore forming stage (FG0) and with a predominance at the one-nucleate (FG1) stage.…”

Section: Discussionmentioning

confidence: 99%

“…The identification of both NLE/RSA4 and MDN1/ REA1 in 60S ribosomal subunit biogenesis in yeast (Galani et al, 2004;Nissan et al, 2002) and their interaction in plants (Chantha and Matton, 2007) and in yeast (Ulbrich et al, 2009) (Chantha et al, 2006). Moreover, the semisterility phenotype was maintained in the following generations.…”

Section: Discussionmentioning

confidence: 99%

“…Biogenesis of the 60S subunit is a highly coordinated process that progress from the nucleolus to the cytoplasm and that involves the participation of more than 70 trans-acting factors for the maturation of ribosomal RNAs and their assembly with ribosomal proteins (Fatica and Tollervey, 2002;Fromont-Racine et al, 2003;Tschochner and Hurt, 2003). Ribosome biogenesis was mostly characterized in yeast but most of the trans-acting factors identified, including MDN1/ REA1 and NLE/RSA4 (Chantha et al, 2006), are well conserved in higher eukaryotes and ribosome biogenesis pathway is therefore expected to be similar in plants, animals and fungi (Fatica and Tollervey, 2002;FromontRacine et al, 2003;Tschochner and Hurt, 2003). The Solanum chacoense MDN1 and NLE proteins were shown to interact together in two-hybrid assays, which brings support to the conservation of 60S subunit biogenesis process in plants (Chantha and Matton, 2007).…”

Section: Discussionmentioning

confidence: 99%

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The MIDASIN and NOTCHLESS genes are essential for female gametophyte development in Arabidopsis thaliana

Chantha

Gray-Mitsumune

Houde

et al. 2010

Physiol Mol Biol Plants

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Female gametophyte development in Arabidopsis thaliana follows a well-defined program that involves many fundamental cellular processes. In this study, we report the involvement of the Arabidopsis thaliana MIDASIN1 (AtMDN1) gene during female gametogenesis through the phenotypic characterization of plants heterozygous for an insertional mdn1 mutant allele. The MDN1 yeast ortholog has previously been shown to encode a non-ribosomal protein involved in the maturation and assembly of the 60S ribosomal subunit. Heterozygous MDN1/mdn1 plants were semisterile and mdn1 allele transmission through the female gametophyte was severely affected. Development of mdn1 female gametophyte was considerably delayed compared to their wild-type siblings. However, delayed mdn1 female gametophytes were able to reach maturity and a delayed pollination experiment showed that a small proportion of the female gametophytes were functional. We also report that the Arabidopsis NOTCHLESS (AtNLE) gene is also required for female gametogenesis. The NLE protein has been previously shown to interact with MDN1 and to be also involved in 60S subunit biogenesis. The introduction of an AtNLE-RNA interference construct in Arabidopsis led to semisterility defects. Defective female gametophytes were mostly arrested at the one-nucleate (FG1) developmental stage. These data suggest that the activity of both AtMDN1 and AtNLE is essential for female gametogenesis progression.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The MIDASIN and NOTCHLESS genes are essential for female gametophyte development in Arabidopsis thaliana

Chantha

Gray-Mitsumune

Houde

et al. 2010

Physiol Mol Biol Plants

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“…Interestingly, two homologs of the Drosophila melanogaster NOTCHLESS gene involved in cell fate decision and developmental processes through the Notch pathway and ribosomal biogenesis were also upregulated from 24 h (NLE and NLE1). NOTCHLESS plant homologs have been shown to regulate cell growth and proliferation (Chantha et al, 2006). Another gene that regulates ribosomal biogenesis (RRS1) also clustered in C3.…”

Section: Protein Biosynthesis Reorientation During Protoplast Culturementioning

confidence: 99%

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Chupeau

Granier

Pichon³

et al. 2013

The Plant Cell

101

109

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The molecular mechanisms underlying plant cell totipotency are largely unknown. Here, we present a protocol for the efficient regeneration of plants from Arabidopsis thaliana protoplasts. The specific liquid medium used in our study leads to a high rate of reentry into the cell cycle of most cell types, providing a powerful system to study dedifferentiation/regeneration processes in independent somatic cells. To identify the early events in the establishment of totipotency, we monitored the genome-wide transcript profiles of plantlets and protoplast-derived cells (PdCs) during the first week of culture. Plant cells rapidly dedifferentiated. Then, we observed the reinitiation and reorientation of protein synthesis, accompanied by the reinitiation of cell division and de novo cell wall synthesis. Marked changes in the expression of chromatin-associated genes, especially of those in the histone variant family, were observed during protoplast culture. Surprisingly, the epigenetic status of PdCs and well-established cell cultures differed, with PdCs exhibiting rare reactivated transposons and epigenetic changes. The differentially expressed genes identified in this study are interesting candidates for investigating the molecular mechanisms underlying plant cell plasticity and totipotency. One of these genes, the plant-specific transcription factor ABERRANT LATERAL ROOT FORMATION4, is required for the initiation of protoplast division.

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Underexpression of the plant NOTCHLESS gene, encoding a WD-repeat protein, causes pleitropic phenotype during plant development

Chantha

Matton

2006

Planta

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WD-repeat proteins are involved in a breadth of cellular processes. While the WD-repeat protein encoding gene NOTCHLESS has been involved in the regulation of the Notch signaling pathway in Drosophila, its yeast homolog Rsa4p was shown to participate in 60S ribosomal subunit biogenesis. The plant homolog ScNLE was previously characterized in Solanum chacoense (ScNLE) as being involved in seed development. However, expression data and reduced size of ScNLE underexpressing plants suggested in addition a role during shoot development. We here report the detailed phenotypic characterization of ScNLE underexpressing plants during shoot development. ScNLE was shown to be expressed in actively dividing cells of the shoot apex. Consistent with this, ScNLE underexpression caused pleiotropic defects such as a reduction in aerial organ size, a reduction in some organ numbers, delayed flowering, and an increase in stomatal index. Analysis of adaxial epidermal cells revealed that both cell number and cell size were reduced in mature leaves of ScNLE underexpressing lines. Two-hybrid screens with the Nle domain and the WD-repeat domain of ScNLE allowed the isolation of homologs of yeast MIDASIN and NSA2 genes, the products of which are involved in 60S ribosomal subunit biogenesis in yeast. A ScNLE-GFP chimeric protein was localized in both the cytoplasm and nucleus. These data altogether suggest that ScNLE likely plays a role in 60S ribosomal subunit biogenesis, which is essential for proper cellular growth and proliferation during plant development.

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Characterization of the plant Notchless homolog, a WD repeat protein involved in seed development

Cited by 18 publications

References 69 publications

The MIDASIN and NOTCHLESS genes are essential for female gametophyte development in Arabidopsis thaliana

The MIDASIN and NOTCHLESS genes are essential for female gametophyte development in Arabidopsis thaliana

Characterization of the Early Events Leading to Totipotency in an Arabidopsis Protoplast Liquid Culture by Temporal Transcript Profiling

Underexpression of the plant NOTCHLESS gene, encoding a WD-repeat protein, causes pleitropic phenotype during plant development

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