Characterization of the Populations of <i>Botrytis cinerea</i> Infecting Plastic Tunnel-Grown Strawberry and Tomato in the Hubei Province of China

Yang, Ruyi; Li, Na; Zhou, Ziliang; Li, Guoqing

doi:10.1094/pdis-01-20-0164-re

Cited by 4 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supporting this was the recent observation that gipsy retrotransposons are associated with the production of small RNAs (sRNAs) that may contribute to pathogenicity (Porquier et al, 2021). This association with sRNAs might explain the prevalence of boty and the variation in association of transposons with pathogenicity across populations (Wagih et al, 2020, Yang, Li, et al, 2020, Simon et al, 2022. While the transposa genotype is between regions, the relative contribution of host and geography is not fully resolvable (Figure 3a) (Diao et al, 2019;Kozhar et al, 2020;Leyronas et al, 2014;Mercier et al, 2019;Plesken et al, 2021;Walker et al, 2014).…”

Section: E X Ten S Ive G Ene Ti C D Iver S It Y Of B Cinere Amentioning

confidence: 96%

“…Supporting this was the recent observation that gipsy retrotransposons are associated with the production of small RNAs (sRNAs) that may contribute to pathogenicity (Porquier et al., 2021 ). This association with sRNAs might explain the prevalence of boty and the variation in association of transposons with pathogenicity across populations (Wagih et al., 2020 , Yang, Li, et al., 2020 , Simon et al., 2022 ). While the transposa genotype is predominant across the global B. cinerea population, the ratios of these variants are dynamic across the past three decades, showing the potential for dynamism and fluctuation in this widespread pathogen (Figure 2c ).…”

Section: Extensive Genetic Diversity Of B Cinereamentioning

confidence: 99%

See 1 more Smart Citation

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Singh,

Caseys,

Kliebenstein

2023

Molecular Plant Pathology

View full text Add to dashboard Cite

Botrytis cinerea Pers. Fr. (teleomorph: Botryotinia fuckeliana) is a necrotrophic fungal pathogen that attacks a wide range of plants. This updated pathogen profile explores the extensive genetic diversity of B. cinerea, highlights the progress in genome sequencing, and provides current knowledge of genetic and molecular mechanisms employed by the fungus to attack its hosts. In addition, we also discuss recent innovative strategies to combat B. cinerea.TaxonomyKingdom: Fungi, phylum: Ascomycota, subphylum: Pezizomycotina, class: Leotiomycetes, order: Helotiales, family: Sclerotiniaceae, genus: Botrytis, species: cinerea.Host rangeB. cinerea infects almost all of the plant groups (angiosperms, gymnosperms, pteridophytes, and bryophytes). To date, 1606 plant species have been identified as hosts of B. cinerea.Genetic diversityThis polyphagous necrotroph has extensive genetic diversity at all population levels shaped by climate, geography, and plant host variation.PathogenicityGenetic architecture of virulence and host specificity is polygenic using multiple weapons to target hosts, including secretory proteins, complex signal transduction pathways, metabolites, and mobile small RNA.Disease control strategiesEfforts to control B. cinerea, being a high‐diversity generalist pathogen, are complicated. However, integrated disease management strategies that combine cultural practices, chemical and biological controls, and the use of appropriate crop varieties will lessen yield losses. Recently, studies conducted worldwide have explored the potential of small RNA as an efficient and environmentally friendly approach for combating grey mould. However, additional research is necessary, especially on risk assessment and regulatory frameworks, to fully harness the potential of this technology.

show abstract

Section: E X Ten S Ive G Ene Ti C D Iver S It Y Of B Cinere Amentioning

confidence: 96%

“…Supporting this was the recent observation that gipsy retrotransposons are associated with the production of small RNAs (sRNAs) that may contribute to pathogenicity (Porquier et al., 2021 ). This association with sRNAs might explain the prevalence of boty and the variation in association of transposons with pathogenicity across populations (Wagih et al., 2020 , Yang, Li, et al., 2020 , Simon et al., 2022 ). While the transposa genotype is predominant across the global B. cinerea population, the ratios of these variants are dynamic across the past three decades, showing the potential for dynamism and fluctuation in this widespread pathogen (Figure 2c ).…”

Section: Extensive Genetic Diversity Of B Cinereamentioning

confidence: 99%

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Singh,

Caseys,

Kliebenstein

2023

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…In addition to maize, wheat has become an important wintering host plant of FAWs [10,11]. Furthermore, there have been reports of FAW infestations in rice fields across many areas of China [12][13][14]. The COI-R Tpi-C configuration, detected using the COI and Tpi (MspI) gene combination identification method, represents the primary host biotype that initially arrived in China [15,16].…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiota Affects Host Fitness of Fall Armyworm Feeding on Different Food Types

Ma,

Wang,

Ren

et al. 2024

Insects

View full text Add to dashboard Cite

The fall armyworm (FAW), Spodoptera frugiperda, seriously threatens food and cash crops. Maize, wheat, and even rice damage by FAWs have been reported in many areas of China. It is urgent to clarify the mechanism which FAWs adapt to different feeding hosts and develop effective control technologies. Two-sex life tables and 16s rDNA sequencing were used to determine the host fitness and gut microbial diversity of FAWs when fed four different food types. Considering the life history parameters, pupa weight, and nutrient utilization indexes, the host fitness of FAWs when fed different food types changed in descending order as follows: artificial diet, maize, wheat, and rice. The gut microbial composition and the diversity of FAWs when fed different food types were significantly different, and those changes were driven by low-abundant bacteria. The gut microbes of FAWs that were fed with maize had the highest diversity. The functions of the gut microbes with significant abundance differences were enriched in nutrient and vitamin metabolism and other pathways that were closely related to host adaptation. Furthermore, we identified five genera (Acinetobacter, Variovorax, Pseudomonas, Bacillus, and Serratia) and one genus (Rahnella) that were positively and negatively correlated with the host fitness, respectively. This study revealed the possible role of gut microbes in the host adaptation of FAWs.

show abstract

First Report of Trichoderma crassum Causing Leaf Spot on Tomato (Solanum lycopersicum) in Ohio

Zhao

Yang

Li³

et al. 2023

Plant Disease

View full text Add to dashboard Cite

Trichoderma is a genus of wood-decaying fungi generally found in soil (Druzhinina and Kubicek 2005). Trichoderma crassum was confirmed to be a sister species to T. virens according to the molecular sequencing results (Chaverri et al. 2003). A foliar disease with ~70% incidence on Solanum lycopersicum was observed in a greenhouse at The Ohio State University (40°0’8’’ north latitude, 83°1’36’’ west longitude), Columbus, United States, in December 2021. On average up to 60% of the leaves per two-month-old tomato plant were infected. Initially, the dark-grey color and irregular spots appeared at the leaf tips. As the disease progressed, the yellow necrotic lesions were observed surrounding the preformed disease spots. Finally, the infected leaves appeared curled and wilted as a whole. The leaf fragments from three tomato plants 40 inches apart were cut from the diseased lesions and surface sterilized with 75% ethanol (30 seconds) and 1% NaOCl (60 seconds), subsequently rinsed with sterilized deionized water three times. Nine pieces of the sterilized leaf tissues were then placed on the PDA plates at 28℃ in the dark and incubated in one incubator for 4 days. The pure cultures of five isolates were acquired and examined with a light microscope. The fungus from all the isolates changed from white to dark green with the radial pattern and profuse sporulation on the PDA. The produced round conidia were observed under a light microscope (Fig S1). The DNA was extracted from two representative isolates which showed the same morphology. The internal transcribed spacer (ITS) region and a conserved fungal rRNA region were amplified using the primers ITS1/ITS4 (5’-TCCGTAGGTGAACCTGCGG-3’ and 5’-TCCTCCGCTTATTGATATGC-3’) (White et al. 1990) and SR6f/SR7r (5’-TGTTACGACTTTTACTT-3’ and 5’-AGTTAAAAAGCTCGTAGTTG-3’) (Hirose et al. 2012), respectively. The PCR products were further sequenced by Sanger sequencing (Table S1). Based on the BLAST results through NCBI website, the ITS sequences of the two isolates were 99% (566/572) and 98% (558/572) identical to Trichoderma crassum DAOM 164916 (EU280067). Their SR sequences both showed 99% (290/293; 289/293) identity to the same strain. The phylogenetic tree was also created with the sequences of ITS region by MEGA software (version 11) (Fig S2). Therefore, the fungus was identified as Trichoderma crassum based on its morphological characteristics (green conidia), Sanger sequencing results, and phylogenetic tree. To complete Koch’s postulates, the 5-mm-diameter fungal agar discs of 7-day-old pure cultures were used for the inoculation on 18 healthy leaves of six tomato cv. M82 plants with two-month-old. The sterile pure PDA discs of the equal size were used for the mock inoculation as a comparison. Fungal plug method was chosen in this study because it had been widely applied to characterization of the fungal pathogens causing leaf spot disease (Pornsuriya et al. 2020; Yang et al. 2021). Five days later, the same symptom as those that occurred on the previously naturally infected tomato plants were observed on all the inoculated leaves (Fig S3A). However, there were no symptoms on the leaves with the mock inoculation. The fungus re-isolated from the symptomatic leaves showed the consistent morphology (dark-green color with radial sporulation) with the original isolates (Fig S3B). Thus, Trichoderma crassum was verified as the causal agent of the foliar disease on Solanum lycopersicum cv. M82 in our greenhouse. To our knowledge, it is the first report of Trichoderma crassum leading to the leaf spot and wilt on tomato in Ohio. The identification of the causal agent lays the groundwork for the development of necessary disease management techniques. We acknowledge the funding support from CFAES Internal Grants Program 2021009.

show abstract

Characterization of the Populations of Botrytis cinerea Infecting Plastic Tunnel-Grown Strawberry and Tomato in the Hubei Province of China

Cited by 4 publications

References 55 publications

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Genetic and molecular landscapes of the generalist phytopathogen Botrytis cinerea

Gut Microbiota Affects Host Fitness of Fall Armyworm Feeding on Different Food Types

First Report of Trichoderma crassum Causing Leaf Spot on Tomato (Solanum lycopersicum) in Ohio

Contact Info

Product

Resources

About