Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila

Kučera, Dan; Pernicová, Iva; Kovalcik, Adriána; Koller, Martin; Müllerová, Lucie; Sedláček, Petr; Mravec, Filip; Nebesářová, Jana; Kalina, Michal; Márová, Ivana; Krzyžánek, Vladislav; Obruča, Stanislav

doi:10.1016/j.biortech.2018.02.062

Cited by 108 publications

(55 citation statements)

References 20 publications

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“…Among many PHA producing Halomonas spp. (Kucera et al, 2018), H. bluephagenesis TD01, as a Gram-negative bacterium, has been reported to engineered for accumulating PHB, copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate (P34HB) and copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV) in high density growth cells via fed batch fermentation within 48 h (Chen and Jiang, 2018;Chen et al, 2017;Fu et al, 2014;Tan et al, 2014). Contamination free, high cell density and high PHA content have long been its attractions that are achieved under oxygen limitation (Ye et al 2018b).…”

Section: Introductionmentioning

confidence: 99%

Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA)

Chen

Qiao

Shuai

et al. 2018

Metabolic Engineering

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Halomonas bluephagenesis has been developed as a platform strain for the next generation industrial biotechnology (NGIB) with advantages of resistances to microbial contamination and high cell density growth (HCD), especially for production of polyhydroxyalkanoates (PHA) including poly(3-hydroxybutyrate) (PHB), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P34HB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). However, little is known about the mechanism behind PHA accumulation under oxygen limitation. This study for the first time found that H. bluephagenesis utilizes NADH instead of NADPH as a cofactor for PHB production, thus revealing the rare situation of enhanced PHA accumulation under oxygen limitation. To increase NADH/NAD ratio for enhanced PHA accumulation under oxygen limitation, an electron transport pathway containing electron transfer flavoprotein subunits α and β encoded by etf operon was blocked to increase NADH supply, leading to 90% PHB accumulation in the cell dry weight (CDW) of H. bluephagenesis compared with 84% by the wild type. Acetic acid, a cost-effective carbon source, was used together with glucose to balance the redox state and reduce inhibition on pyruvate metabolism, resulting in 22% more CDW and 94% PHB accumulation. The cellular redox state changes induced by the addition of acetic acid increased 3HV ratio in its copolymer PHBV from 4% to 8%, 4HB in its copolymer P34HB from 8% to 12%, respectively, by engineered H. bluephagenesis. The strategy of systematically modulation on the redox potential of H. bluephagenesis led to enhanced PHA accumulation and controllable monomer ratios in PHA copolymers under oxygen limitation, reducing energy consumption and scale-up complexity.

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Section: Introductionmentioning

confidence: 99%

Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA)

Chen

Qiao

Shuai

et al. 2018

Metabolic Engineering

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“…KM-1 [50,144] Halomonas bluephagenesis TD01 [51] Halomonas nitroreducens [145] Halomonas sp. O-1 [101] Halomonas elongata [101] Halomonas halophila [73] Halomonas marina [53] Halomonas maura [146] Halomonas ventosae [147] Halomonas halodenitrificans [148] Halomonas halodeneurihalina [148] Halomonas salina [148] Halomonas sp. SF2003 [102] Halomonas profundus [54] Halomonas campisalis [56] Halomonas hydrothermalis [77] Vibrio Vibrio proteolyticus [55] Yangia Yangia sp.…”

Section: Genus Species Referencesmentioning

confidence: 99%

“…Spent coffee grounds, the primary waste by-product of coffee processing industry, are still an unusual substrate for valorization. Halomonas halophila was capable of utilizing hydrolysates of spent coffee grounds to produce 61.95% (wt) of PHB [73]. Furthermore, H. halphila showed the ability to utilize molasses, cheese whey hydrolysates, sawdust hydrolysates, and twice diluted corn stover hydrolysate to produce up to 64% (wt) PHB.…”

Section: Low-cost Substrate Usage By Halophilic Bacteriamentioning

confidence: 99%

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

et al. 2020

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Plastic pollution is a severe threat to our environment which necessitates implementation of bioplastics to realize sustainable development for a green world. Polyhydroxyalkanoates (PHA) represent one of the potential candidates for these bioplastics. However, a major challenge faced by PHA is the high production cost which limits its commercial application. Halophiles are considered to be a promising cell factory for PHA synthesis due to its several unique characteristics including high salinity requirement preventing microbial contamination, high intracellular osmotic pressure allowing easy cell lysis for PHA recovery, and capability to utilize wide spectrum of low-cost substrates. Optimization of fermentation parameters has made it plausible to achieve large-scale production at low cost by using halophiles. Further deeper insights into halophiles have revealed the existence of diversified and even novel PHA synthetic pathways within different halophilic species that greatly affects PHA type. Thus, precise metabolic engineering of halophiles with the help of advanced tools and strategies have led to more efficient microbial cell factory for PHA production. This review is an endeavour to summarize the various research achievements in these areas which will help the readers to understand the current developments as well as the future efforts in PHA research.

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“…In this context, the use of extremophiles isolated from challenging habitats, and halophile microbes isolated from salt lakes or the sea is strongly increasing these days in order to use them as work horses in simple, flexible, and stable cultivation setups, which can be operated in an energy-efficient manner due to the need for only restricted sterility precautions. [42][43][44][45][46] Moreover, the use of different bioreactor systems, operated continuously or discontinuously, was exhaustively reviewed recently, indicating that bioreactor setup, process regime, and feeding strategy have major impact on PHA productivity, composition, and microstructure. 47 F i g .…”

Section: Chemical and Biochemical Engineering Approaches In Manufactumentioning

confidence: 99%

“…This finding allows fine-tuning molecular mass by triggering the salt concentration. 44 As shown by Lv and colleagues, clustered regularly interspaced short palindromic repeats interference (CRISPRi) can be used to trigger poly(3HBco-4HB) biosynthesis in E. coli by parallel fine-tuning gene expression and regulating the expression of multiple genes. For this purpose, a pathway was constructed in E. coli for poly(3HB-co-4HB) biosynthesis from glucose.…”

Section: -Hydroxybutyrate (4hb) Containing Scl-phamentioning

confidence: 99%

Chemical and Biochemical Engineering Approaches in Manufacturing Polyhydroxyalkanoate (PHA) Biopolyesters of Tailored Structure with Focus on the Diversity of Building Blocks

Koller¹

2019

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHA) constitute prokaryotic storage materials not only harnessing microbial cells with benefits for survival under challenging environmental conditions, but also attracting attention as biological materials with properties resembling those of currently used thermoplasts and elastomers of petrochemical origin. Strongly dependent on their monomeric composition and microstructure, PHA´s exact material properties are predestined in statu nascendi, hence, during their biosynthesis. The present review sheds light on established and emerging strategies to produce differently composed PHA homo-, co-, ter-, and quarterpolyesters from the groups of short-, medium-, and long-chain PHA. It is shown how microbial strain selection, sophisticated genetic strain engineering based on synthetic biology approaches, advanced feeding strategies, and smart process engineering can be implemented to generate PHA of tailored monomeric composition, microstructure, molar mass, and molar mass distribution. Tailoring these parameters offers the possibility to produce customer-oriented PHA for various purposes, such as packaging materials, carriers of pharmaceutically active compounds, implants, or other emerging fields of use.

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Characterization of the promising poly(3-hydroxybutyrate) producing halophilic bacterium Halomonas halophila

Cited by 108 publications

References 20 publications

Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA)

Engineering NADH/NAD+ ratio in Halomonas bluephagenesis for enhanced production of polyhydroxyalkanoates (PHA)

Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory

Chemical and Biochemical Engineering Approaches in Manufacturing Polyhydroxyalkanoate (PHA) Biopolyesters of Tailored Structure with Focus on the Diversity of Building Blocks

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