Characterization of the Promoter of the Homeobox Gene CaHB12 in Coffea arabica

Nobres, Priscilla; Patreze, Camila Maistro; Waltenberg, Fernanda P.; Correa, Marcelo Favareto; Tavano, Eveline Carla da Rocha; Mendes, Beatriz Madalena Januzzi; Alves‐Ferreira, Márcio

doi:10.1007/s12042-016-9159-2

Cited by 6 publications

(3 citation statements)

References 52 publications

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“…In the drought treatment by air drying (air-dried), a set of plates harboring 20 seedlings of each Arabidopsis transgenic line remained open for 6, 12 or 24 h ( Rodrigues et al , 2012 ; Nobres et al , 2016 ). Meanwhile, a set of plates with control plants remained closed.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

et al. 2017

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Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene glycol (PEG) and salt stress treatments. After 24 h of air-dried and PEG treatments, the pGAL-2kb led to an increase in GUS expression in leaf and root samples when compared to the control samples. These results were corroborated by qPCR expression analysis of the uidA gene. The pGAL-1kb showed no difference in GUS activity between control and treated samples. The pGAL-2kb promoter was evaluated in transgenic soybean roots, leading to an increase in EGFP expression under air-dried treatment. Our data indicates that pGAL-2kb could be a useful tool in developing drought-tolerant cultivars by driving gene expression.

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Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

et al. 2017

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“…However, compared with AtRD29 , some specific inducible promoters can achieve higher levels of expression. An example is the promoter of the Coffea arabica CaHB1 2 gene [20]. For this reason, the identification, isolation, and characterization of new specific promoters inducible by abiotic stress have been crucial to ensure the successful application of gene modification and, consequently, the development of new cultivars resistant to water deprivation stress.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

et al. 2019

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BackgroundDrought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses.ResultsThis study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment.ConclusionsThe full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.

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“…In coffee, most studies evaluating organ/tissue-specific promoters have focused on seed-and fruit-specific genes (Marraccini et al 1999;Cotta et al 2014). Other examples include the promoter regions of genes involved in stress and defense responses (Brandalise et al 2009;Severino et al 2012;Petitot et al 2013;Nobres et al 2016;Alves et al 2017), in light-regulated carbon fixation (Marraccini et al 2003) and in caffeine biosynthesis (Satyanarayana et al 2005). In practice, however, the 35S promoter is still the preferred choice for the construction of expression cassettes employed in coffee biotechnology (Mishra and Slater 2012).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

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Barsalobres-Cavallari

et al. 2018

Plant Cell Rep

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A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5'-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.

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Characterization of the Promoter of the Homeobox Gene CaHB12 in Coffea arabica

Cited by 6 publications

References 52 publications

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

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