Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B

Zhao, Guang‐Rong; Zhang, Heng-Ming; Ye, Ting-Xiang; Xiang, Zhi-Jun; Yuan, Ying‐Jin; Guo, Zhi‐Xin; Zhao, Long

doi:10.1016/j.fct.2007.06.034

Cited by 289 publications

(162 citation statements)

References 33 publications

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“…They lyse blood clot, dilate coronary arteries, inhibit platelet aggregation, scavenge free radicals, improve microcirculation and have anti-inflammatory properties. 6,7) Both of them are widely used in clinic for treatment of cardiovascular diseases. Although widely used, their therapeutic benefits are severely limited due to weak activities.…”

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confidence: 99%

A Novel Danshensu-Tetramethylpyrazine Conjugate DT-010 Provides Cardioprotection through the PGC-1α/Nrf2/HO-1 Pathway

Zhang¹,

Hu²,

Luo³

et al. 2017

Biological & Pharmaceutical Bulletin

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In this study, we investigated the cardioprotective mechanisms of action of DT-010, a novel danshensutetramethylpyrazine conjugate. DT-010 significantly preserved cell viability and suppressed cell apoptosis in H9c2 cells injured by tert-butylhydroperoxide (t-BHP), iodoacetic acid (IAA) and hypoxia-reoxygenation. In addition, DT-010 pre-treatment reduced the intracellular level of free radicals including superoxide anion (·O 2 ), hydroxyl radical (·OH) and peroxynitrite anion (ONOO ) after t-BHP exposure. Moreover, DT-010 up-regulated the protein expression of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) and nuclear factor-E2-related factor 2 (Nrf2) as well as mitochondrial transcription factor A (Tfam) and heme oxygenase-1 (HO-1) in H9c2 cells. DT-010 also triggered Nrf2 nuclear translocation. In a rat myocardial ischemia-reperfusion model, DT-010 significantly alleviated myocardial infarction. The results indicated that DT-010 may be a promising candidate for the treatment of cardiovascular diseases, particularly myocardial ischemia and reperfusion injury.Key words Danshensu derivative; oxidative stress; apoptosis; myocardial ischemia reperfusion injury; peroxisome proliferator-activated receptor gamma coactivator 1 alpha/nuclear factor-E2-related factor 2/heme oxygenase-1 (PGC-1α/Nrf2/HO-1) pathway Myocardial ischemia is a primary cause of sudden death worldwide. Myocardial ischemia results from a decreased blood flow to the heart, preventing the heart from receiving adequate supply of energy. The reduced blood flow is usually caused by a partial or complete blockade of the arteries. Traditional treatments focus on restoring blood supply, such as by pass-graft surgery, dilation of coronary artery and coronary thrombolysis, and etc. 1) Although restoration of blood flow to the ischemic heart tissues is important, it is not adequate because myocardial ischemic damage is progressively developed following reperfusion due to an over-production of a large number of biospecies. 2,3) Compelling evidence demonstrated that free radicals generated during ischemia and reperfusion play pivotal roles in myocardial apoptosis. Free radical scavengers can effectively inhibit myocardial apoptosis. The previous studies have demonstrated that agents that combine anti-thrombolytic and anti-oxidative activities showed promising therapeutic efficacy in the treatment of myocardial ischemia. 4,5) In China, many traditional herbs have been used to prevent and treat myocardial ischemia. Among them are Salvia miltiorrhiza (Danshen) and Ligusticum wallichii (Chuanxiong). As the major active ingredients, (3-(3,4-dihydroxyphenyl) lactic acid) (Danshensu, DSS, Fig. 1) isolated from Danshen and tetramethylpyrazine (TMP, Fig. 1) isolated from Chuanxiong have a variety of biological activities. They lyse blood clot, dilate coronary arteries, inhibit platelet aggregation, scavenge free radicals, improve microcirculation and have anti-inflammatory properties. 6,7) Both of them are widely used in clinic for treatm...

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confidence: 99%

A Novel Danshensu-Tetramethylpyrazine Conjugate DT-010 Provides Cardioprotection through the PGC-1α/Nrf2/HO-1 Pathway

Zhang¹,

Hu²,

Luo³

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Its main active ingredients are water-soluble materials, including danshensu, salvianolic acid, and protocatechualdehyde (Cui et al, 2007). These compounds can protect endothelial cells, and prevent inflammation, lipid peroxidation, and calcium overload (Kim et al, 2005;Li et al, 2006;Ding and Yuan, 2007;Lam et al, 2007;Zhao et al, 2008). Studies have proved that Salvia miltiorrhiza can mitigate the pathological changes in the liver, lung, kidney, intestine, and stomach of SAP rats, and prevent the occurrence of multiple organ failure (Zhang et al, 2002a;2002b;2002c;2005).…”

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confidence: 99%

Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis

Zhang

Chengjun

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods: The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results: The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions: Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.

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“…Salvia miltiorrhiza (Zhao et al, 2008), Origanum vulgare (Fujie et al, 2003), and Mentha haplocalyx (She et al, 2010), whereas, to the best of our knowledge, its presence in Lavandula species has not been previously reported.…”

Section: Dla Has Been Found In a Number Of Lamiaceae Plants Such Asmentioning

confidence: 65%

Antidepressant-Like Effects of 3-(3,4-dihydroxyphenyl)lactic Acid Isolated from Lavender (Lavandula angustifolia) Flowers in Mice

Ueno¹,

Matsui²,

Masuda³

et al. 2014

FSTR

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Material and MethodsAnimals Six-week-old male ICR mice (29 _ 31 g) were obtained from Japan SLC (Hamamatsu, Japan). The mice were group-housed (4 mice/cage; cage size: 210 × 315 × 130 mm height) in a room maintained at a constant temperature (22 _ 24℃) and humidity (38 _ 51%) with a 12-h light-dark cycle (light:7:00 _ 19:00). They were fed a standard diet, CRF-1 (Oriental Yeast, Tokyo, Japan), and tap water ad libitum, and allowed to acclimatize for 7 days. On the last day of the acclimatization T. Ueno et al. 1214 period, the mice were divided into groups comprising eight mice each. Behavioral tests were performed between 13:00 and 17:00 h Table 1. Isolation and identification of 3-(3,4-dihydroxyphenyl)lactic acid (DLA)The LE prepared from 125 g of dried lavender flowers as described above was suspended in 1.75 L of 0.1% formic acid, chromatographed on a porous resin column (Sepabeads SP-70;Mitsubishi Chemical, Tokyo, Japan), and eluted with 1.75 L of 0.1% formic acid twice and then 1.75 L of 10% ethanol in 0.1 % formic acid three times. The second 10% ethanol fraction was evaporated in vacuo and lyophilized to give 961 mg of the crude product. The crude product was purified by prep-HPLC under similar conditions to that described above to yield 297 mg of DLA:

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Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B

Cited by 289 publications

References 33 publications

A Novel Danshensu-Tetramethylpyrazine Conjugate DT-010 Provides Cardioprotection through the PGC-1α/Nrf2/HO-1 Pathway

A Novel Danshensu-Tetramethylpyrazine Conjugate DT-010 Provides Cardioprotection through the PGC-1α/Nrf2/HO-1 Pathway

Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis

Antidepressant-Like Effects of 3-(3,4-dihydroxyphenyl)lactic Acid Isolated from Lavender (Lavandula angustifolia) Flowers in Mice

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