Characterization of the RcsC→YojN→RcsB Phosphorelay Signaling Pathway Involved in Capsular Synthesis in Escherichia coli

Chen, Mian Hua; Takeda, Shin‐ichiro; Yamada, H.; Ishii, Yuko; Yamashino, Takafumi; Mizuno, Takeshi

doi:10.1271/bbb.65.2364

Cited by 61 publications

(49 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RscC phosphorylates the intermediary IM protein RcsD, which then phosphorylates the cytoplasmic response regulator RcsB. 106,107 Phosphorylated RcsB can bind DNA as a homodimer or as a heterodimer with the unstable accessory cytoplasmic protein RcsA or with some other accessory regulators. 20,108 Target genes affect many processes including cell division, osmoregulation, cell envelope homeostasis, motility and virulence.…”

Section: The Rcs Esrmentioning

confidence: 99%

Regulation of bacterial virulence gene expression by cell envelope stress responses

Flores-Kim

Darwin

2014

Virulence

View full text Add to dashboard Cite

Section: The Rcs Esrmentioning

confidence: 99%

Regulation of bacterial virulence gene expression by cell envelope stress responses

Flores-Kim

Darwin

2014

Virulence

View full text Add to dashboard Cite

“…However, many reports suggest that the Rcs system might sense alterations of the cell envelope. The Rcs system has been reported to be activated by environmental signals, such as desiccation, osmotic shock and Zn 2+ concentration (Hagiwara et al, 2003;Ophir & Gutnick, 1994;Sledjeski & Gottesman, 1996), in the presence of mutations affecting cell envelope components, such as tol (Clavel et al, 1996;Mouslim & Groisman, 2003), rfa (Parker et al, 1992), mdoH (Ebel et al, 1997), dsbA and dsbB (El-Kazzaz et al, 2004), or after overproduction of envelope proteins, such as TolB/Pal (Majdalani et al, 2002), RcsF (Gervais & Drapeau, 1992), DjlA (Toutain et al, 2003), LolA and OmpG (Chen et al, 2001). …”

Section: Introductionmentioning

confidence: 99%

Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis

et al. 2005

View full text Add to dashboard Cite

Curli are necessary for the adherence of Escherichia coli to surfaces, and to each other, during biofilm formation, and the csgBA and csgDEFG operons are both required for their synthesis. A recent survey of gene expression in Pseudomonas aeruginosa biofilms has identified tolA as a gene activated in biofilms. The tol genes play a fundamental role in maintaining the outer-membrane integrity of Gram-negative bacteria. RcsC, the sensor of the RcsBCD phosphorelay, is involved, together with RcsA, in colanic acid capsule synthesis, and also modulates the expression of tolQRA and csgDEFG. In addition, the RcsBCD phosphorelay is activated in tol mutants or when Tol proteins are overexpressed. These results led the authors to investigate the role of the tol genes in biofilm formation in laboratory and clinical isolates of E. coli. It was shown that the adherence of cells was lowered in the tol mutants. This could be the result of a drastic decrease in the expression of the csgBA operon, even though the expression of csgDEFG was slightly increased under such conditions. It was also shown that the Rcs system negatively controls the expression of the two csg operons in an RcsA-dependent manner. In the tol mutants, activation of csgDEFG occurred via OmpR and was dominant upon repression by RcsB and RcsA, while these two regulatory proteins repressed csgBA through a dominant effect on the activator protein CsgD, thus affecting curli synthesis. The results demonstrate that the Rcs system, previously known to control the synthesis of the capsule and the flagella, is an additional component involved in the regulation of curli. Furthermore, it is shown that the defect in cell motility observed in the tol mutants depends on RcsB and RcsA. INTRODUCTIONThe ability of bacteria to recognize and adhere to a specific surface is a fundamental aspect of microbial ecology and pathogenesis. Bacterial adhesins and fimbriae confer specific recognition and adhesion to diverse target molecules, such as mammalian host tissue components or inorganic materials. Curli are highly adhesive proteinaceous fibres involved in this process that are produced by Escherichia coli (Vidal et al., 1998) and Salmonella (Collinson et al., 1991). The genes necessary for curli formation are clustered in the csgBA and csgDEFG operons. CsgA encodes the curlin subunit, CsgB is thought to nucleate CsgA curlin fibres (Bian & Normark, 1997), CsgD is a transcriptional activator of the csgBA operon, and CsgE, CsgF and CsgG are three putative curli assembly factors. The lipoprotein CsgG localizes to the inner leaflet of the outer membrane and may serve as a curli assembly platform (Loferer et al., 1997); CsgE and CsgF are thought to be chaperone-like proteins (Chapman et al., 2002). The csg genes appear to be expressed in environmental strains, clinical isolates and some laboratory strains of E. coli.Regulation of curli synthesis is carried out through a complex network of interactions between the transcription factors and the csg regulatory region. Nine regulators have ...

show abstract

“…A widely studied two-component signaling system is the RcsCDB signaling system, first identified in Escherichia coli as a regulator of the production of the colanic acid capsule (53). This system can be activated by diverse envelope-related stresses (13,14,19,20,35,36,47,49,52,61) or the overproduction of proteins such as DjlA, LolA, and OmpG (11,37).…”

mentioning

confidence: 99%

Genome Expression Analyses Revealing the Modulation of the Salmonella Rcs Regulon by the Attenuator IgaA

Mariscotti

Portillo

2009

J Bacteriol

View full text Add to dashboard Cite

Intracellular growth attenuator A (IgaA) was identified as a Salmonella enterica regulator limiting bacterial growth inside fibroblasts. Genetic evidence further linked IgaA to repression of the RcsCDB regulatory system, which responds to envelope stress. How IgaA attenuates this system is unknown. Here, we present genome expression profiling data of S. enterica serovar Typhimurium igaA mutants grown at high osmolarity and displaying exacerbated Rcs responses. Transcriptome data revealed that IgaA attenuates gene expression changes requiring phosphorylated RcsB (RcsBϳP) activity. Some RcsB-regulated genes, yciGFE and STM1862 (pagO)-STM1863-STM1864, were equally expressed in wild-type and igaA strains, suggesting a maximal expression at low levels of RcsBϳP. Other genes, such as metB, ypeC, ygaC, glnK, glnP, napA, glpA, and nirB, were shown for the first time and by independent methods to be regulated by the RcsCDB system. Interestingly, IgaA-deficient strains with reduced RcsC or RcsD levels exhibited different Rcs responses and distinct virulence properties. spv virulence genes were differentially expressed in most of the analyzed strains. spvA expression required RcsB and IgaA but, unexpectedly, was also impaired upon stimulation of the RcsC3 RcsD3RcsB phosphorelay. Overproduction of either RcsB ؉ or a nonphosphorylatable RcsB(D56Q) variant in strains displaying low spvA expression unveiled that both dephosphorylated RcsB and RcsBϳP are required for optimal spvA expression. Taken together, our data support a model with IgaA attenuating the RcsCDB system by favoring the switch of RcsBϳP to the dephosphorylated state. This role of IgaA in constantly fine-tuning the RcsBϳP/RcsB ratio may ensure the proper expression of important virulence factors, such as the Spv proteins.

show abstract

Characterization of the RcsC→YojN→RcsB Phosphorelay Signaling Pathway Involved in Capsular Synthesis in Escherichia coli

Cited by 61 publications

References 11 publications

Regulation of bacterial virulence gene expression by cell envelope stress responses

Regulation of bacterial virulence gene expression by cell envelope stress responses

Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis

Genome Expression Analyses Revealing the Modulation of the Salmonella Rcs Regulon by the Attenuator IgaA

Contact Info

Product

Resources

About