Characterization of the segmental transport mechanisms of DL-methionine hydroxy analogue along the intestinal tract of rainbow trout with an additional comparison to DL-methionine

To, Van Pham Thi Ha; Subramaniam, Marina; Masagounder, Karthik; Loewen, Matthew E.

doi:10.1016/j.cbpa.2020.110776

Cited by 7 publications

(3 citation statements)

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“…Finally, the most conceivable explanation could be that MHA is not efficiently uptaken by these cells. Nevertheless, when the expression of MCTs proposed to be in charge of intestinal MHA flux [ 11 , 12 ] were assessed in the two hepatic cell lines, all of them were detected at mRNA levels. To date, it cannot be excluded that post-transcriptional/translational regulations, together with post-translational modifications, are critical for MCTs activities.…”

Section: Discussionmentioning

confidence: 99%

“…At the molecular level, one possible explanation would be that both molecules are not uptaken by the same transporters. MHA absorption likely occurs via monocarboxylate transporters (MCTs) rather than canonical AA transporters, as proposed in other species [ 11 ] but also in RT gut tissues [ 12 ]. Such differences in MET source absorptions due to fish species or MET forms might impair the supply of MET-related pathways, which control part of anabolic and catabolic processes responsible for the healthy growth of organisms.…”

Section: Introductionmentioning

confidence: 99%

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Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

Pinel

Héraud

Morin

et al. 2022

IJMS

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The replacement of fishmeal by plant proteins in aquafeeds imposes the use of synthetic methionine (MET) sources to balance the amino acid composition of alternative diets and so to meet the metabolic needs of fish of agronomic interest such as rainbow trout (RT-Oncorhynchus mykiss). Nonetheless, debates still exist to determine if one MET source is more efficiently used than another by fish. To address this question, the use of fish cell lines appeared a convenient strategy, since it allowed to perfectly control cell growing conditions notably by fully depleting MET from the media and studying which MET source is capable to restore cell growth/proliferation and metabolism when supplemented back. Thus, results of cell proliferation assays, Western blots, RT-qPCR and liquid chromatography analyses from two RT liver-derived cell lines revealed a better absorption and metabolization of DL-MET than DL-Methionine Hydroxy Analog (MHA) with the activation of the mechanistic Target Of Rapamycin (mTOR) pathway for DL-MET and the activation of integrated stress response (ISR) pathway for MHA. Altogether, the results clearly allow to conclude that both synthetic MET sources are not biologically equivalent, suggesting similar in vivo effects in RT liver and, therefore, questioning the MHA efficiencies in other RT tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

Pinel

Héraud

Morin

et al. 2022

IJMS

Self Cite

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“…As an organic acid salt rather than an amino acid, MHA exhibits lower water solubility when compared to crystalline methionine, although it cannot be utilized by animals until it is converted into L-methionine after a series of metabolic transformations. In addition, there are distinguished differences between crystalline methionine and MHA in absorption, transport and metabolism in vivo as the result of their different chemical properties ( Maenz and Engele-Schaan, 1996 ; Pham Thi Ha To et al, 2020 ; To et al, 2021 ). Recent research has shown that the transport of L-methionine relies on multiple apical sodium-dependent/independent high/low-affinity transporters, while the transport of MHA is mediated by proton-dependent monocarboxylate transporters of the SLC16 family and apical sodium monocarboxylate transporters of the SLC5 family ( To et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei)

et al. 2023

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An 8-week feeding trial was conducted to evaluate the effects of L-methionine and methionine hydroxy analogue calcium (MHA-Ca) supplements in low-fishmeal diet on growth performance, hepatopancreas morphology, protein metabolism, anti-oxidative capacity, and immunity of Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isoenergetic diets were designed: PC (203.3 g/kg fishmeal), NC (100 g/kg fishmeal), MET (100 g/kg fishmeal +3 g/kg L-methionine) and MHA-Ca (100 g/kg fishmeal +3 g/kg MHA-Ca). White shrimp (initial body weight 0.23 ± 0.00 g, 50 shrimp per tank) were allocated to 12 tanks and divided among 4 treatments in triplicates. In response to L-methionine and MHA-Ca supplementations, the shrimp exhibited higher weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), and lower hepatosomatic index (HSI) compared to those fed the NC diet (p < 0.05). The WGR and SGR of shrimp fed L-methionine and MHA-Ca showed no difference with those in the PC diet (p > 0.05). Both of L-methionine and MHA-Ca supplementary diets significantly decreased the malondialdehyde (MDA) levels of shrimp when compared with the NC diet (p < 0.05). L-methionine supplementation improved the lysozyme (LZM) activity and total antioxidant capacity (T-AOC) of shrimp, while the MHA-Ca addition elevated the reduced glutathione (GSH) levels in comparison with those fed the NC diet (p < 0.05). Hypertrophied blister cells in hepatocytes were observed in shrimp fed the NC diet, and alleviated with L-methionine and MHA-Ca supplementations. Shrimp fed the MET and MHA-Ca diets had higher mRNA expression levels of target of rapamycin (tor) than those fed the NC diet (p < 0.05). Compared to the NC group, dietary MHA-Ca supplementation upregulated the expression level of cysteine dioxygenase (cdo) (p < 0.05), while L-methionine supplementation had no significant impact (p > 0.05). The expression levels of superoxide dismutase (sod) and glutathione peroxidase (gpx) were significantly upregulated by L-methionine supplemented diet in comparison with those in the NC group (p < 0.05). Overall, the addition of both L-methionine and MHA-Ca elevated the growth performance, facilitated protein synthesis, and ameliorated hepatopancreatic damage induced by plant-protein enriched diet in L. vannamei. L-methionine and MHA-Ca supplements enhanced anti-oxidants differently.

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Absorption of methionine sources in animals—is there more to know?

Becquet¹,

Vázquez-Añón²,

Mercier³

et al. 2023

Animal Nutrition

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Characterization of the segmental transport mechanisms of DL-methionine hydroxy analogue along the intestinal tract of rainbow trout with an additional comparison to DL-methionine

Cited by 7 publications

References 82 publications

Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

Are the Main Methionine Sources Equivalent? A Focus on DL-Methionine and DL-Methionine Hydroxy Analog Reveals Differences on Rainbow Trout Hepatic Cell Lines Functions

Comparative study on the effects of crystalline L-methionine and methionine hydroxy analogue calcium supplementations in the diet of juvenile Pacific white shrimp (Litopenaeus vannamei)

Absorption of methionine sources in animals—is there more to know?

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